Galasso, H. L., Richard, M., Lefebvre, S., Aliaume, C., & Callier, M. D. (2018). Body size and temperature effects on standard metabolic rate for determining metabolic scope for activity of the polychaete Hediste (Nereis) diversicolor. *PeerJ*, *6*, e5675.

Considering the ecological importance and potential value of Hediste diversicolor, a better understanding of its metabolic rate and potential growth rates is required. The aims of this study are: (i) to describe key biometric relationships; (ii) to test the effects of temperature and body size on standard metabolic rate (as measure by oxygen consumption) to determine critical parameters, namely Arrhenius temperature (T-A), allometric coefficient (b) and reaction rate; and (iii) to determine the metabolic scope for activity (MSA) of H. diversicolor for further comparison with published specific growth rates. Individuals were collected in a Mediterranean lagoon (France). After 10 days of acclimatization, 7 days at a fixed temperature and 24 h of fasting, resting oxygen consumption rates (VO2) were individually measured in the dark at four different temperatures (11, 17, 22 and 27 degrees C) in worms weighing from 4 to 94 mgDW (n = 27 per temperature). Results showed that DW and L3 were the most accurate measurements of weight and length, respectively, among all the metrics tested. Conversion of WW (mg), DW (mg) and L3 (mm) were quantified with the following equations: DW = 0.15 x WW, L3 = 0.025 x TL(mm) + 1.44 and DW = 0.8 x L3(3.68). Using an equation based on temperature and allometric effects, the allometric coefficient (b) was estimated at 0.8 for DW and at 2.83 for L3. The reaction rate (VO2) equaled to 12.33 mu mol gDW(-1) h(-1) and 0.05 mu mol mm L3(-1) h(-)(1) at the reference temperature (20 degrees C, 293.15 K). Arrhenius temperature (T-A) was 5,707 and 5,664 K (for DW and L3, respectively). Metabolic scope for activity ranged from 120.1 to 627.6 J gDW(-1) d(-1). Predicted maximum growth rate increased with temperature, with expected values of 7-10% in the range of 15-20 degrees C. MSA was then used to evaluate specific growth rates (SGR) in several experiments. This paper may be used as a reference and could have interesting applications in the fields of aquaculture, ecology and biogeochemical processes.