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Auteur (up) Bodin, N.; Lucas, V.; Dewals, P.; Adeline, M.; Esparon, J.; Chassot, E.
Titre Effect of brine immersion freezing on the determination of ecological tracers in fish Type Article scientifique
Année 2014 Publication Revue Abrégée European Food Research and Technology
Volume 238 Numéro 6 Pages 1057-1062
Mots-Clés Brine immersion; Fatty acids; Frozen storage; Large pelagic fish; Persistent organic pollutants; Stable isotopes
Résumé The use of a multi-ecological tracer approach provides valuable and complementary insights to investigate the complex biology and ecology of large pelagic fish. Brine immersion freezing is the most common preservation technique used onboard for large fish to be frozen whole until they are delivered for sale and processing. We evaluated the effect of brine freezing on lipid and fatty acid composition, C and N stable isotope ratios, and organochlorine contaminant levels of yellowfin tuna (Thunnus albacares) and skipjack tuna (Katsuwonus pelamis). Fresh tunas were stored in a saturated sodium chlorine brine immersion tank maintained at -20 A degrees C for 6 weeks, and ecological tracers were analysed on dorsal muscle samples collected before and after brine freezing. No significant effect of the fish preservation technique was found except for delta N-15 whose signatures slightly increased after a 6-week period of brine immersion. Because N isotopic shift was close to the analytical precision and probably related to a higher risk of salt penetration in small tunas with abraded skin, we consider our results as conservative and conclude that ecological tracers can indeed be analysed on brine-freezing-preserved tunas.
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ISSN 1438-2377 ISBN Médium
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Numéro d'Appel LL @ pixluser @ collection 353
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Auteur (up) Bodin, N.; Tapie, N.; Le Menach, K.; Chassot, E.; Elie, P.; Rochard, E.; Budzinski, H.
Titre PCB contamination in fish community from the Gironde Estuary (France) : blast from the past Type Article scientifique
Année 2014 Publication Revue Abrégée Chemosphere
Volume 98 Numéro Pages 66-72
Mots-Clés Bioindicators; Biomagnification; Fish assemblage; Food safety; Persistent organic pollutants (POPs)
Résumé The contamination of the Gironde Estuary, southwest of France, by polychlorinated biphenyls (PCBs) was assessed using six fish of high ecological and economic importance as bioindicator species. The concentrations of 21 PCB congeners and total fat contents were determined in the muscle and liver of eels (Anguilla anguilla), seabass (Dicentrarchus labrax), flounders (Platichthys flesus), meagres (Argyrosomus regius), mullets (Liza ramada), and soles (Solea vulgaris). In addition, information regarding the trophic ecology of the studied fish was obtained through the analysis of carbon and nitrogen stable isotopes (i.e., delta C-13 and delta N-15) in muscle. Results revealed high PCB concentrations in fish compared to monitored European estuaries. The muscle of eels was by far the most contaminated fish flesh (Sigma(7)PCBs = 1000 +/- 440 ng g(-1), on a dry weight basis), while the higher PCB concentrations in liver were measured in flounder (Sigma(7)PCBs = 2040 +/- 1160 ng g(-1), d.w.). A quantile regression approach allowed to investigate the fate of PCBs in the Gironde estuarine fish assemblage, and revealed a general process of trophic magnification. Finally, most of the analysed fish presented PCB concentrations in muscle meat above the current European maximum limits for sea products, while the derived “Toxic Equivalent Quantity” (TEQ) revealed human health concerns only for high-fat fish consumption.
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Langue Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0045-6535 ISBN Médium
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Numéro d'Appel LL @ pixluser @ collection 326
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Auteur (up) Bonhommeau, S.; Nieblas, A.E.; Chassot, E.; Kaplan, D.; Dubroca, L.; Manacorda, C.; Barde, J.; Le Pape, O.
Titre Reply to Feeley and Machovina : trophic ecology complements estimates of land use change due to food production Type Article scientifique
Année 2014 Publication Revue Abrégée Proceedings of the National Academy of Sciences of the United States of America
Volume 111 Numéro 9 Pages
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Auteur institutionnel Thèse
Editeur Lieu de Publication Éditeur
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Volume de collection Numéro de collection Edition
ISSN 0027-8424 ISBN Médium
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Numéro d'Appel LL @ pixluser @ collection 354
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Auteur (up) BORDIER, C.; SARAUX, C.; VIBLANC, V.A.; GACHOT-NEVEU, H.; BEAUGEY, M.; LE MAHO, Y.; LE BOHEC, C.
Titre Inter-Annual Variability of Fledgling Sex Ratio in King Penguins Type Article scientifique
Année 2014 Publication Revue Abrégée Plos One
Volume 9 Numéro 12 Pages 1-17
Mots-Clés
Résumé As the number of breeding pairs depends on the adult sex ratio in a monogamous species with biparental care, investigating sex-ratio variability in natural populations is essential to understand population dynamics. Using 10 years of data (20002009) in a seasonally monogamous seabird, the king penguin (Aptenodytes patagonicus), we investigated the annual sex ratio at fledging, and the potential environmental causes for its variation. Over more than 4000 birds, the annual sex ratio at fledging was highly variable (ranging from 44.4% to 58.3% of males), and on average slightly biased towards males (51.6%). Yearly variation in sex-ratio bias was neither related to density within the colony, nor to global or local oceanographic conditions known to affect both the productivity and accessibility of penguin foraging areas. However, rising sea surface temperature coincided with an increase in fledging sex-ratio variability. Fledging sex ratio was also correlated with difference in body condition between male and female fledglings. When more males were produced in a given year, their body condition was higher (and reciprocally), suggesting that parents might adopt a sex-biased allocation strategy depending on yearly environmental conditions and/or that the effect of environmental parameters on chick condition and survival may be sex-dependent. The initial bias in sex ratio observed at the juvenile stage tended to return to 1:1 equilibrium upon first breeding attempts, as would be expected from Fisher's classic theory of offspring sex-ratio variation.
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Langue Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 1932-6203 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 1126
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Auteur (up) Boudour-Boucheker, N.; Boulo, V.; Charmantier-Daures, M.; Grousset, E.; Anger, K.; Charmantier, G.; Lorin-Nebel, C.
Titre Differential distribution of V-type H+-ATPase and Na+/K+-ATPase in the branchial chamber of the palaemonid shrimp Macrobrachium amazonicum Type Article scientifique
Année 2014 Publication Revue Abrégée Cell and Tissue Research
Volume 357 Numéro 1 Pages 195-206
Mots-Clés Branchiostegite; Gills; Na+/K+-ATPase; Osmoregulation; V-type H+-ATPase; crab eriocheir-sinensis; decapoda; epithelial potential difference; fresh-water crab; gill epithelium; homarus-gammarus; ion-transport; larval development; lobster; olfersii; plasma-membrane; salinity acclimation
Résumé V-H+-ATPase and Na+/K+-ATPase were localized in the gills and branchiostegites of M. amazonicum and the effects of salinity on the branchial chamber ultrastructure and on the localization of transporters were investigated. Gills present septal and pillar cells. In freshwater (FW), the apical surface of pillar cells is amplified by extensive evaginations associated with mitochondria. V-H+-ATPase immunofluorescence was localized in the membranes of the apical evaginations and in clustered subapical areas of pillar cells, suggesting labeling of intracellular vesicle membranes. Na+/K+-ATPase labeling was restricted to the septal cells. No difference in immunostaining was recorded for both proteins according to salinity (FW vs. 25 PSU). In the branchiostegite, both V-H+-ATPase and Na+/K+-ATPase immunofluorescence were localized in the same cells of the internal epithelium. Immunogold revealed that V-H+-ATPase was localized in apical evaginations and in electron-dense areas throughout the inner epithelium, while Na+/K+-ATPase occurred densely along the basal infoldings of the cytoplasmic membrane. Our results suggest that morphologically different cell types within the gill lamellae may also be functionally specialized. We propose that, in FW, pillar cells expressing V-H+-ATPase absorb ions (Cl-, Na+) that are transported either directly to the hemolymph space or through a junctional complex to the septal cells, which may be responsible for active Na+ delivery to the hemolymph through Na+/K+-ATPase. This suggests a functional link between septal and pillar cells in osmoregulation. When shrimps are transferred to FW, gill and branchiostegite epithelia undergo ultrastructural changes, most probably resulting from their involvement in osmoregulatory processes.
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Langue English Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 0302-766x ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 541
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