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Auteur Boudour-Boucheker, N.; Boulo, V.; Charmantier-Daures, M.; Grousset, E.; Anger, K.; Charmantier, G.; Lorin-Nebel, C.
Titre Differential distribution of V-type H+-ATPase and Na+/K+-ATPase in the branchial chamber of the palaemonid shrimp Macrobrachium amazonicum Type Article scientifique
Année 2014 Publication Revue Abrégée (up) Cell and Tissue Research
Volume 357 Numéro 1 Pages 195-206
Mots-Clés Branchiostegite; Gills; Na+/K+-ATPase; Osmoregulation; V-type H+-ATPase; crab eriocheir-sinensis; decapoda; epithelial potential difference; fresh-water crab; gill epithelium; homarus-gammarus; ion-transport; larval development; lobster; olfersii; plasma-membrane; salinity acclimation
Résumé V-H+-ATPase and Na+/K+-ATPase were localized in the gills and branchiostegites of M. amazonicum and the effects of salinity on the branchial chamber ultrastructure and on the localization of transporters were investigated. Gills present septal and pillar cells. In freshwater (FW), the apical surface of pillar cells is amplified by extensive evaginations associated with mitochondria. V-H+-ATPase immunofluorescence was localized in the membranes of the apical evaginations and in clustered subapical areas of pillar cells, suggesting labeling of intracellular vesicle membranes. Na+/K+-ATPase labeling was restricted to the septal cells. No difference in immunostaining was recorded for both proteins according to salinity (FW vs. 25 PSU). In the branchiostegite, both V-H+-ATPase and Na+/K+-ATPase immunofluorescence were localized in the same cells of the internal epithelium. Immunogold revealed that V-H+-ATPase was localized in apical evaginations and in electron-dense areas throughout the inner epithelium, while Na+/K+-ATPase occurred densely along the basal infoldings of the cytoplasmic membrane. Our results suggest that morphologically different cell types within the gill lamellae may also be functionally specialized. We propose that, in FW, pillar cells expressing V-H+-ATPase absorb ions (Cl-, Na+) that are transported either directly to the hemolymph space or through a junctional complex to the septal cells, which may be responsible for active Na+ delivery to the hemolymph through Na+/K+-ATPase. This suggests a functional link between septal and pillar cells in osmoregulation. When shrimps are transferred to FW, gill and branchiostegite epithelia undergo ultrastructural changes, most probably resulting from their involvement in osmoregulatory processes.
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ISSN 0302-766x ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 541
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Auteur Bodin, N.; Tapie, N.; Le Menach, K.; Chassot, E.; Elie, P.; Rochard, E.; Budzinski, H.
Titre PCB contamination in fish community from the Gironde Estuary (France) : blast from the past Type Article scientifique
Année 2014 Publication Revue Abrégée (up) Chemosphere
Volume 98 Numéro Pages 66-72
Mots-Clés Bioindicators; Biomagnification; Fish assemblage; Food safety; Persistent organic pollutants (POPs)
Résumé The contamination of the Gironde Estuary, southwest of France, by polychlorinated biphenyls (PCBs) was assessed using six fish of high ecological and economic importance as bioindicator species. The concentrations of 21 PCB congeners and total fat contents were determined in the muscle and liver of eels (Anguilla anguilla), seabass (Dicentrarchus labrax), flounders (Platichthys flesus), meagres (Argyrosomus regius), mullets (Liza ramada), and soles (Solea vulgaris). In addition, information regarding the trophic ecology of the studied fish was obtained through the analysis of carbon and nitrogen stable isotopes (i.e., delta C-13 and delta N-15) in muscle. Results revealed high PCB concentrations in fish compared to monitored European estuaries. The muscle of eels was by far the most contaminated fish flesh (Sigma(7)PCBs = 1000 +/- 440 ng g(-1), on a dry weight basis), while the higher PCB concentrations in liver were measured in flounder (Sigma(7)PCBs = 2040 +/- 1160 ng g(-1), d.w.). A quantile regression approach allowed to investigate the fate of PCBs in the Gironde estuarine fish assemblage, and revealed a general process of trophic magnification. Finally, most of the analysed fish presented PCB concentrations in muscle meat above the current European maximum limits for sea products, while the derived “Toxic Equivalent Quantity” (TEQ) revealed human health concerns only for high-fat fish consumption.
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Numéro d'Appel LL @ pixluser @ collection 326
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Auteur Minguez, L.; Farcy, E.; Ballandonne, C.; Lepailleur, A.; Serpentini, A.; Lebel, J.M.; Bureau, R.; Halm-Lemeille, M.P.
Titre Acute toxicity of 8 antidepressants: What are their modes of action? Type Article scientifique
Année 2014 Publication Revue Abrégée (up) Chemosphere
Volume 108 Numéro Pages 314-319
Mots-Clés Daphnia magna; Haliotis tuberculata; Lysosomotropism; Multi-approach; Narcosis; Pharmaceutical; assessment; cationic amphiphilic drugs; cause foot detachment; cell-cultures; cytotoxicity; ecotoxicology; fluoxetine; hemocytes; human pharmaceuticals; pi interactions; water
Résumé Currently, the hazard posed by pharmaceutical residues is a major concern of ecotoxicology. Most of the antidepressants belong to a family named the Cationic Amphipathic Drugs known to have specific interactions with cell membranes. The present study assessed the impact of eight antidepressants belonging to selective serotonin reuptake inhibitors or serotonin norepinephrine reuptake inhibitors by the combination of multi-approaches (in vivo, in vitro, in silico) and gives some insights on the mode of action for these molecules. Antidepressants were from the most to the least toxic compound for Daphnia magna: Sertraline (EC50 = 1.15 mg L-1) > Clomipramine (2.74 mg L-1)> Amitriptyline (4.82 mg L-1)> Fluoxetine (5.91 mg L-1) > Paroxetine (6.24 mg L-1) > Mianserine (7.81 mg L-1) > Citalopram (30.14 mg L-1) and Venlafaxine (141.28 mg L-1). These acute toxicities were found correlated to Log K, coefficients (R = 0.93, p < 0.001) and to cytotoxicity assessed on abalone hemocytes through the neutral red uptake assay (R = 0.96, p < 0.001). If narcosis as mode of action is typically expected during acute ecotoxicity bioassays, we showed by molecular modeling that particular interactions can exist between antidepressants and phosphatidylcholine, a major component of cell membranes, leading to a more specific mode of action corresponding to a potential acidic hydrolysis of ester functions. (C) 2014 Elsevier Ltd. All rights reserved.
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 440
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Auteur Hadjadji, I.; Frehi, H.; Ayada, L.; Abadie, E.; Collos, Y.
Titre A comparative analysis of Alexandrium catenella/tamarense blooms in Annaba Bay (Algeria) and Thau lagoon (France); phosphorus limitation as a trigger Type Article scientifique
Année 2014 Publication Revue Abrégée (up) Comptes Rendus Biologies
Volume 337 Numéro 2 Pages 117-122
Mots-Clés Algeria; Algérie; Efflorescences; France; Méditerranée; alexandrium catenella/tamarense; blooms; mediterranean
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ISSN 1631-0691 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 397
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Auteur Cooke, S.J.; Killen, S.S.; Metcalfe, J.D.; McKenzie, D.J.; Mouillot, D.; Jørgensen, C.; Peck, M.A.
Titre Conservation physiology across scales: insights from the marine realm Type Article scientifique
Année 2014 Publication Revue Abrégée (up) Conservation Physiology
Volume 2 Numéro 1 Pages
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Résumé As the field of conservation physiology develops and becomes increasingly integrated with ecology and conservation science, the fundamental concept of scale is being recognized as important, particularly for ensuring that physiological knowledge is contextualized in a manner most relevant to policy makers, conservation practitioners and stakeholders. Failure to consider the importance of scale in conservation physiology—both the challenges and the opportunities that it creates—will impede the ability of this discipline to generate the scientific understanding needed to contribute to meaningful conservation outcomes. Here, we have focused on five aspects of scale: biological, spatial, temporal, allometric and phylogenetic. We also considered the scale of policy and policy application relevant to those five types of scale as well as the merits of upscaling and downscaling to explore and address conservation problems. Although relevant to all systems (e.g. freshwater, terrestrial) we have used examples from the marine realm, with a particular emphasis on fishes, given the fact that there is existing discourse regarding scale and its relevance for marine conservation and management. Our synthesis revealed that all five aspects of scale are relevant to conservation physiology, with many aspects inherently linked. It is apparent that there are both opportunities and challenges afforded by working across scales but, to understand mechanisms underlying conservation problems, it is essential to consider scale of all sorts and to work across scales to the greatest extent possible. Moreover, given that the scales in biological processes will often not match policy and management scales, conservation physiology needs to show how it is relevant to aspects at different policy/management scales, change the scales at which policy/management intervention is applied or be prepared to be ignored.
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 504
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