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Auteur (up) Lamarre, S.G.; Ditlecadet, D.; McKenzie, D.J.; Bonnaud, L.; Driedzic, W.R. url  doi
  Titre Mechanisms of protein degradation in mantle muscle and proposed gill remodeling in starved Sepia officinalis Type Article scientifique
  Année 2012 Publication Revue Abrégée Am. J. Physiol.-Regul. Integr. Comp. Physiol.  
  Volume 303 Numéro 4 Pages R427-R437  
  Mots-Clés Rna; cathepsin; cephalopod; cephalopods; gadus-morhua l; growth; metabolic enzymes; metabolism; octopus-vulgaris; polyubiquitin; proteasome; rainbow-trout; skeletal-muscle; squid; starvation; triglyceride; trout oncorhynchus-mykiss  
  Résumé Lamarre SG, Ditlecadet D, McKenzie DJ, Bonnaud L, Driedzic WR. Mechanisms of protein degradation in mantle muscle and proposed gill remodeling in starved Sepia officinalis. Am J Physiol Regul Integr Comp Physiol 303: R427-R437, 2012. First published May 30, 2012; doi:10.1152/ajpregu.00077.2012.-Cephalopods have relatively high rates of protein synthesis compared to rates of protein degradation, along with minimal carbohydrate and lipid reserves. During food deprivation on board protein is catabolized as a metabolic fuel. The aim of the current study was to assess whether biochemical indices of protein synthesis and proteolytic mechanisms were altered in cuttlefish, Sepia officinalis, starved for 7 days. In mantle muscle, food deprivation is associated with a decrease in protein synthesis, as indicated by a decrease in the total RNA level and dephosphorylation of key signaling molecules, such as the eukaryote binding protein, 4E-BP1 (regulator of translation) and Akt. The ubiquitination-proteasome system (UPS) is activated as shown by an increase in the levels of proteasome beta-subunit mRNA, polyubiquitinated protein, and polyubiquitin mRNA. As well, cathepsin activity levels are increased, suggesting increased proteolysis through the lysosomal pathway. Together, these mechanisms could supply amino acids as metabolic fuels. In gill, the situation is quite different. It appears that during the first stages of starvation, both protein synthesis and protein degradation are enhanced in gill. This is based upon increased phosphorylation of 4E-BP1 and enhanced levels of UPS indicators, especially 20S proteasome activity and polyubiquitin mRNA. It is proposed that an increased protein turnover is related to gill remodeling perhaps to retain essential hemolymph-borne compounds.  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0363-6119 ISBN Médium  
  Région Expédition Conférence  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 702  
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