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Auteur (up) Caro, A.; Escalas, A.; Bouvier, C.; Grousset, E.; Lautredou-Audouy, N.; Roques, C.; Charmantier, M.; Gros, O. url  doi
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  Titre Epibiotic bacterial community of Sphaeroma serratum (Crustacea, Isopoda): relationship with molt status Type Article scientifique
  Année 2012 Publication Revue Abrégée Mar. Ecol.-Prog. Ser.  
  Volume 457 Numéro Pages 11-27  
  Mots-Clés 16s ribosomal-rna; Crustacean; DGGE band pattern; Epibiotic biofilm; Molt cycle; Sphaeroma; fish; gastropod; gradient gel-electrophoresis; hydrothermal-vent; in-situ hybridization; mid-atlantic ridge; oxidizing bacteria; phylogenetic diversity; riftia-pachyptila; shrimp rimicaris-exoculata; urothoe-poseidonis  
  Résumé Sphaeroma serratum is a marine isopod species that inhabits seashores from Europe to West Africa. The individuals live under stones in direct contact with reduced sediments and harbour a diverse bacterial community on the cuticle of their pleopods. We investigated the diversity of these epibiotic bacteria on male (pubescent and senescent) and female specimens with electron microscopic observations and molecular tools. The microbial community of S. serratum was shown to be composed of at least 5 bacterial morphotypes observed on the pleopodal cuticle in all male specimens. Using fluorescence in situ hybridization, we identified 5 major phylogenetic groups (alpha-, beta-, gamma- and delta-Proteobacteria and Archaea) whereas denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments of epibiotic bacteria revealed 50 bands. The bacterial community associated with S. serratum seems more diverse than in other marine crustaceans, such as Rimicaris. The relative diversity of this bacterial community was also studied in relation to the molt cycle. The comparison of DGGE band patterns of several individuals from female, pubescent male and senescent male groups revealed that the bacterial community diversity was dependent on the sex and the age of the individuals and more generally on the molt status.  
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  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
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  ISSN 0171-8630 ISBN Médium  
  Région Expédition Conférence  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 564  
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