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Auteur (up) van Lis, R.; Brugiere, S.; Baffert, C.; Coute, Y.; Nitschke, W.; Atteia, A. doi  openurl
  Titre Hybrid cluster proteins in a photosynthetic microalga Type Article scientifique
  Année 2019 Publication Revue Abrégée Febs J.  
  Volume Numéro Pages  
  Mots-Clés angstrom; Chlamydomonas; chlamydomonas-reinhardtii; desulfovibrio-vulgaris hildenborough; desulfuricans atcc-27774; escherichia-coli; hcp; metalloprotein; nitrate; nitric-oxide; prismane protein; proteomics; redox; spectroscopy; x-ray structures  
  Résumé Hybrid cluster proteins (HCPs) are metalloproteins characterized by the presence of an iron-sulfur-oxygen cluster. These proteins occur in all three domains of life. In eukaryotes, HCPs have so far been found only in a few anaerobic parasites and photosynthetic microalgae. With respect to all species harboring an HCP, the green microalga Chlamydomonas reinhardtii stands out by the presence of four HCP genes. The study of the gene and protein structures as well as the phylogenetic analyses strongly support a model in which the HCP family in the alga has emerged from a single gene of alpha proteobacterial origin and then expanded by several rounds of duplications. The spectra and redox properties of HCP1 and HCP3, produced heterologously in Escherichia coli, were analyzed by electron paramagnetic resonance spectroscopy on redox-titrated samples. Both proteins contain a [4Fe-4S]-cluster as well as a [4Fe-2O-2S]-hybrid cluster with paramagnetic properties related to those of HCPs from Desulfovibrio species. Immunoblotting experiments combined with mass spectrometry-based proteomics showed that both nitrate and darkness contribute to the strong upregulation of the HCP levels in C. reinhardtii growing under oxic conditions. The link to the nitrate metabolism is discussed in the light of recent data on the potential role of HCP in S-nitrosylation in bacteria.  
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  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 1742-464x ISBN Médium  
  Région Expédition Conférence  
  Notes WOS:000484247300001 Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2644  
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