bascule de visibilité Search & Display Options

Tout Sélectionner    Désélectionner
 |   | 
Détails
   print
  Enregistrement Liens
Auteur (up) Suquet, M.; Gourtay, C.; Donval, A.; Le Goic, N.; Quere, C.; Malo, F.; Le Grand, J.; Ratiskol, D.; Mingant, C.; Fauvel, C. doi  openurl
  Titre The quality of great scallop (Pecten maximus) sperm after thawing Type Article scientifique
  Année 2016 Publication Revue Abrégée Gen. Comp. Endocrinol.  
  Volume 229 Numéro Pages 127-131  
  Mots-Clés aquaculture; crassostrea-gigas; Cryopreservation; cryopreserved spermatozoa; fertility; fertilization; fucata-martensii; Gamete; japanese pearl oyster; motility; ostrea-edulis; pacific oyster; Pecten maximus; reproduction; Sperm  
  Résumé Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm samples were obtained after intragonadal injection of serotonin and cryopreserved using a previously published protocol. Sperm quality was assessed using a panel of four parameters: sperm motility characteristics, using a computer assisted sperm analysis plugin with Image J, intracellular ATP content using an ATP-Lite kit, sperm integrity, using flow cytometry and sperm morphology, using transmission electron microscopy. For each parameter, fresh (control) and thawed spermatozoa were compared. A significant decrease of both the percentage of motile spermatozoa (reduction: 75%) and sperm swimming speed (86%) were observed for thawed sperm compared with fresh sperm. The percentage of living spermatozoa, as assessed using flow cytometry, was significantly lower for thawed sperm (72.4 +/- 2.5%) compared with fresh sperm (86.4 +/- 1.1). However, no significant difference of intracellular sperm ATP content was observed between fresh and thawed sperm. Post thawing, while some spermatozoa showed little or no morphological differences compared with fresh sperm, others had undergone drastic changes, including swelling of the plasma membrane, structural alterations of the chromatin and damage to mitochondria. In conclusion, the descriptive parameters studied in the present work showed that the quality of thawed great scallop sperm was lower than that of fresh cells but was still sufficient for use in aquaculture programs and sperm cryobanking for this species. (c) 2016 Elsevier Inc. All rights reserved.  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0016-6480 ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 1657  
Lien permanent pour cet enregistrement
Tout Sélectionner    Désélectionner
 |   | 
Détails
   print

Save Citations:
Export Records: