bascule de visibilité Search & Display Options

Tout Sélectionner    Désélectionner
 |   | 
  Enregistrement Liens
Auteur (up) Ituarte, R.B.; Lignot, J.-H.; Charmantier, G.; Spivak, E.; Lorin-Nebel, C. doi  openurl
  Titre Immunolocalization and expression of Na+/K+ -ATPase in embryos, early larval stages and adults of the freshwater shrimp Palaemonetes argentinus (Decapoda, Caridea, Palaemonidae) Type Article scientifique
  Année 2016 Publication Revue Abrégée Cell Tissue Res.  
  Volume 364 Numéro 3 Pages 527-541  
  Mots-Clés Antennal gland; antennal glands; branchial chamber; crayfish astacus-leptodactylus; dorsal organ; Extrabranchial organs; Gill; h+-atpase; hemigrapsus-crenulatus; kinetic characterization; lobster homarus-gammarus; Ontogeny; Osmoregulation; osmoregulatory organs; prawn macrobrachium-rosenbergii  
  Résumé The euryhaline shrimp Palaemonetes argentinus exemplifies an evolutionary transition from brackish to freshwater habitats that requires adequate osmoregulatory capacities. Hyperosmoregulation is functional at hatching and it likely begins during the embryonic phase allowing this species to develop entirely in fresh water. Here, we investigated the Na+/K+-ATPase alpha-subunit gene (nka-alpha) expression using quantitative real-time PCR and localized Na+/K+-ATPase (NKA) in ion-transporting epithelia through immunofluorescence microscopy. We reared shrimps from spawning to juvenile stages at two salinities (1, 15 aEuro degrees) and maintained adults for 3 weeks at three salinity treatments (1, 15, 25 aEuro degrees). nka-alpha gene expression was measured in: (1) embryos at an early (SI), intermediate (SII) and late (SIII) stage of embryonic development; (2) newly hatched larvae (Zoea I, ZI); and (3) isolated gill tissue of adults. The nka-alpha expression was low in SI and SII embryos and reached maximum levels prior to hatching (SIII), which were similar to expression levels detected in the ZI. The nka-alpha expression in SIII and ZI was highest at 15 aEuro degrees, whereas salinity did not affect expression in earlier embryos. In SIII, in ZI and in a later zoeal stage ZIV, NKA was localized in epithelial cells of pleurae, in the inner-side epithelium of branchiostegite and in the antennal glands. Gills appeared in the ZIV but NKA immunolabeling of the cells of the gill shaft occurred in a subsequent developmental larval stage, the decapodid. Extrabranchial organs constitute the main site of osmoregulation in early ontogenetic stages of this freshwater shrimp.  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0302-766x ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 1621  
Lien permanent pour cet enregistrement
Tout Sélectionner    Désélectionner
 |   | 

Save Citations:
Export Records: