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Auteur van der Geest, M.; van Gils, J.A.; van der Meer, J.; Olff, H.; Piersma, T.
Titre Suitability of calcein as an in situ growth marker in burrowing bivalves Type Article scientifique
Année 2011 Publication (up) Revue Abrégée Journal of Experimental Marine Biology and Ecology
Volume 399 Numéro Pages 1-7
Mots-Clés Daily growth rate in situ fluorescent marking Intertidal mudflat Lucinidae Mark-and-recapture Seagrass banc-darguin marking mauritania scallop oxytetracycline abundance shells rates fish age
Résumé The fluorochrome calcein has been used in numerous growth studies of molluscs to internally mark calcified structures. Because of interspecific variations in marking success and possible effects on growth performance, methodological assessments of the suitability of calcein as a growth marker, especially in field contexts, remain necessary. Here we report on the effects of different calcein concentrations (100, 200, 400 and 800 mg l(-1) on fluorescent mark deposition, growth rate, density, body condition and size-frequency distribution of an intertidal infaunal bivalve species, Loripes lacteus (Linnaeus, 1758), using an outdoor immersion technique. To avoid stress caused by handling and transportation, in situ enclosures were placed at seagrass-covered patches during low tide, to which calcein solutions were added. After a 1.3- to 2.6-h period of exposure to calcein, the enclosures were removed. Sites were sampled three months later. All calcein concentrations produced live L. lacteus with a clear fluorescent shell mark, but the percentage successfully marked tended to increase with higher calcein concentrations. Furthermore, marking success and growth rate decreased significantly with shell size (i.e., age). Calcein concentration did not measurably affect shell growth rate, body condition and size-frequency distribution, but the numerical densities were lower for sites treated with calcein concentrations >= 400 mg l(-1). Our results suggest that in situ calcein-marking of burrowing lucinid bivalves with low concentrations (100-200 mg l(-1)) is a non-invasive and rapid method to determine growth rate, provided that the lucinids are not too old. (c) 2011 Elsevier B.V. All rights reserved.
Adresse [van der Geest, Matthijs; van Gils, Jan A.; van der Meer, Jaap; Piersma, Theunis] NIOZ Royal Netherlands Inst Sea Res, Dept Marine Ecol, NL-1790 AB Den Burg, Texel, Netherlands. [van der Meer, Jaap] Free Univ Amsterdam, Dept Theoret Biol, NL-1081 HV Amsterdam, Netherlands. [Olff, Han] Univ Groningen, Ctr Ecol & Evolutionary Sci CEES, Community & Conservat Ecol Grp, NL-9700 CC Groningen, Netherlands. [Piersma, Theunis] Univ Groningen, Ctr Ecol & Evolutionary Sci CEES, Anim Ecol Grp, NL-9700 CC Groningen, Netherlands. van der Geest, M (reprint author), NIOZ Royal Netherlands Inst Sea Res, Dept Marine Ecol, POB 59, NL-1790 AB Den Burg, Texel, Netherlands Matthijs.van.der.Geest@nioz.nl
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Langue English Langue du Résumé Titre Original
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Notes ISI Document Delivery No.: 745QC Times Cited: 0 Cited Reference Count: 33 van der Geest, Matthijs van Gils, Jan A. van der Meer, Jaap Olff, Han Piersma, Theunis Nwo-wotro[w.] We thank the authorities of the Parc National du Banc d'Arguin (PNBA) for the opportunity to carry out this research on the intertidal flats of PNBA and for the use of facilities at the Iwik field station. Logistical help from Lemhaba Ould Yarba in Nouakchott is much appreciated. We also thank the inhabitants of the Biological Station in Iwik, especially Sidi Ely, Sall Mamdou Alassane and Ahmedou Ould Lameime for their cheerful contribution and co-operation. We are grateful to Tamar Lok and two anonymous referees for constructive comments. We thank Henrike Andresen for help with the development of the calcein-marking method and Amrit Cado van der Lelij who made a large contribution to the work in the laboratory. Joana Cardoso helped with shell preparation. We are grateful to the staff of Laboratoire de Sclerochronologie des Animaux Aquatiques (LASAA) for their assistance with making Fig. 1B and C. Allert Bijleveld, Geert Aarts and all participants of the NIOZ April 2010 Mixed-Effect modeling course of Alain Zuur and Elena N. Ieno are thanked for their constructive comments on statistics. Dick Visser produced the final figures. This study was financed by NWO-WOTRO Integrated Programme grant W. to TP. [ST] Elsevier science bv Amsterdam Approuvé pas de
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