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Auteur (up) Alfonso, S.; Blanc, M.; Joassard, L.; Keiter, S.H.; Munschy, C.; Loizeau, V.; Begout, M.-L.; Cousin, X.
Titre Examining multi- and transgenerational behavioral and molecular alterations resulting from parental exposure to an environmental PCB and PBDE mixture Type Article scientifique
Année 2019 Publication Revue Abrégée Aquat. Toxicol.
Volume 208 Numéro Pages 29-38
Mots-Clés adult zebrafish; Behavior; brominated flame retardants; developmental exposure; dietary-exposure; embryonic exposure; Epigenetic; Gene transcription; global dna methylation; left-right asymmetry; Offspring; Parental exposure; polybrominated diphenyl ethers; polychlorinated-biphenyls pcbs; Zebrafish; zebrafish danio-rerio
Résumé Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants extensively used during the 20th century and still present in aquatic environments despite their ban. Effects of exposure to these compounds over generations are poorly documented. Therefore, our aims were to characterize behavioral responses and underlying molecular mechanisms in zebrafish exposed to an environmentally relevant mixture of PCBs and PBDEs as well as in four unexposed offspring generations. Zebrafish (F0) were chronically exposed from the first meal onward to a diet spiked with a mixture containing 22 PCB and 7 PBDE congeners in proportions and concentrations reflecting environmental situations (Sigma PCBs = 1991 and Sigma PBDEs = 411 ng/g). Four offspring generations (F1 to F4) were obtained from this F0 and were not further exposed. Behavior was assessed at both larval and adult stages. Mechanisms related to behavioral defects (habenula maturation and c-fos transcription) and methylation (dnmts transcription) were monitored in larvae. Exposed adult F0 as well as F1 and F3 adults displayed no behavioral change while F2 expressed anxiety-like behavior. Larval behavior was also disrupted, Le. hyperactive after light to dark transition in F1 or hypoactive in F2, F3 and F4. Behavioral disruptions may be related to defect in habenula maturation (observed in F1) and change in c-fos transcription (observed in F1 and F2). Transcription of the gene encoding DNA methyltransferase (dnmt3ba) was also modified in all generations. Our results lead us to hypothesize that chronic dietary exposure to an environmentally relevant mixture of PCB and PBDE triggers multigenerational and transgenerational molecular and behavioral disruptions in a vertebrate model.
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ISSN 0166-445x ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2549
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Auteur (up) Benhaim, D.; Ferrari, S.; Colchen, T.; Chatain, B.; Begout, M.-L.
Titre Relationship between individual and group learning in a marine teleost: A case study with sea bass under self-feeding conditions Type Article scientifique
Année 2017 Publication Revue Abrégée Learn Behav.
Volume 45 Numéro 3 Pages 276-286
Mots-Clés animal groups; Behavior; dicentrarchus-labrax; example; fish; Group conditions; growth; Juveniles; Operant conditioning; Personality traits; Positive reinforcement; Producer-scrounger; producer-scrounger game; Social structure; traits; zebrafish danio-rerio
Résumé Fish learning and cognition are usually approached by testing single individuals in various devices such as mazes that have serious drawbacks, especially in gregarious species, including the stress induced by the test procedure. This might impair the results and lead to misinterpretation about the learning abilities of the targeted species. In order to provide an alternative to the individual-based tests, we investigated for the first time the operant conditioning of four similar groups (50 individuals per tank) of sea bass. We used two computerized self-feeder devices per tank, each coupled with individual electronic identification and that were alternately activated during varying positive appetitive reinforcement period of time (7 to 1 day). Learning abilities were examined at both group and individual levels. At the group level, the operant conditioning was demonstrated as the triggering activity significantly decreased when the device was turned off and increased when it was turned on, whatever the reinforcement period duration. The individual level analysis revealed a more complex situation with fish showing different learning performances that can be best explained through the producer-scrounger game theory.
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Volume de collection Numéro de collection Edition
ISSN 1543-4494 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2191
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Auteur (up) Legradi, J.B.; Di Paolo, C.; Kraak, M.H.S.; van der Geest, H.G.; Schymanski, E.L.; Williams, A.J.; Dingemans, M.M.L.; Massei, R.; Brack, W.; Cousin, X.; Begout, M.-L.; van der Oost, R.; Carion, A.; Suarez-Ulloa, V.; Silvestre, F.; Escher, B.I.; Engwall, M.; Nilen, G.; Keiter, S.H.; Pollet, D.; Waldmann, P.; Kienle, C.; Werner, I.; Haigis, A.-C.; Knapen, D.; Vergauwen, L.; Spehr, M.; Schulz, W.; Busch, W.; Leuthold, D.; Scholz, S.; vom Berg, C.M.; Basu, N.; Murphy, C.A.; Lampert, A.; Kuckelkorn, J.; Grummt, T.; Hollert, H.
Titre An ecotoxicological view on neurotoxicity assessment Type Article scientifique
Année 2018 Publication Revue Abrégée Environ. Sci Eur.
Volume 30 Numéro Pages 46
Mots-Clés Behaviour; zebrafish danio-rerio; environmental risk-assessment; acetylcholinesterase inhibitors; adverse outcome pathways; aop; Computational toxicity; developmental neurotoxicity; diesel exhaust; Eco-neurotoxicity; Ecological; eda; effect-directed analysis; lateral-line; Neurotoxicity; performance liquid-chromatography; reach; Species; swimming behavior
Résumé The numbers of potential neurotoxicants in the environment are raising and pose a great risk for humans and the environment. Currently neurotoxicity assessment is mostly performed to predict and prevent harm to human populations. Despite all the efforts invested in the last years in developing novel in vitro or in silico test systems, in vivo tests with rodents are still the only accepted test for neurotoxicity risk assessment in Europe. Despite an increasing number of reports of species showing altered behaviour, neurotoxicity assessment for species in the environment is not required and therefore mostly not performed. Considering the increasing numbers of environmental contaminants with potential neurotoxic potential, eco-neurotoxicity should be also considered in risk assessment. In order to do so novel test systems are needed that can cope with species differences within ecosystems. In the field, online-biomonitoring systems using behavioural information could be used to detect neurotoxic effects and effect-directed analyses could be applied to identify the neurotoxicants causing the effect. Additionally, toxic pressure calculations in combination with mixture modelling could use environmental chemical monitoring data to predict adverse effects and prioritize pollutants for laboratory testing. Cheminformatics based on computational toxicological data from in vitro and in vivo studies could help to identify potential neurotoxicants. An array of in vitro assays covering different modes of action could be applied to screen compounds for neurotoxicity. The selection of in vitro assays could be guided by AOPs relevant for eco-neurotoxicity. In order to be able to perform risk assessment for eco-neurotoxicity, methods need to focus on the most sensitive species in an ecosystem. A test battery using species from different trophic levels might be the best approach. To implement eco-neurotoxicity assessment into European risk assessment, cheminformatics and in vitro screening tests could be used as first approach to identify eco-neurotoxic pollutants. In a second step, a small species test battery could be applied to assess the risks of ecosystems.
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Editeur Lieu de Publication Éditeur
Langue English Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 2190-4715 ISBN Médium
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Numéro d'Appel MARBEC @ alain.herve @ collection 2485
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