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Auteur Lamarre, S.G.; Ditlecadet, D.; McKenzie, D.J.; Bonnaud, L.; Driedzic, W.R.
Titre Mechanisms of protein degradation in mantle muscle and proposed gill remodeling in starved Sepia officinalis Type Article scientifique
Année 2012 Publication (up) Revue Abrégée Am. J. Physiol.-Regul. Integr. Comp. Physiol.
Volume 303 Numéro 4 Pages R427-R437
Mots-Clés Rna; cathepsin; cephalopod; cephalopods; gadus-morhua l; growth; metabolic enzymes; metabolism; octopus-vulgaris; polyubiquitin; proteasome; rainbow-trout; skeletal-muscle; squid; starvation; triglyceride; trout oncorhynchus-mykiss
Résumé Lamarre SG, Ditlecadet D, McKenzie DJ, Bonnaud L, Driedzic WR. Mechanisms of protein degradation in mantle muscle and proposed gill remodeling in starved Sepia officinalis. Am J Physiol Regul Integr Comp Physiol 303: R427-R437, 2012. First published May 30, 2012; doi:10.1152/ajpregu.00077.2012.-Cephalopods have relatively high rates of protein synthesis compared to rates of protein degradation, along with minimal carbohydrate and lipid reserves. During food deprivation on board protein is catabolized as a metabolic fuel. The aim of the current study was to assess whether biochemical indices of protein synthesis and proteolytic mechanisms were altered in cuttlefish, Sepia officinalis, starved for 7 days. In mantle muscle, food deprivation is associated with a decrease in protein synthesis, as indicated by a decrease in the total RNA level and dephosphorylation of key signaling molecules, such as the eukaryote binding protein, 4E-BP1 (regulator of translation) and Akt. The ubiquitination-proteasome system (UPS) is activated as shown by an increase in the levels of proteasome beta-subunit mRNA, polyubiquitinated protein, and polyubiquitin mRNA. As well, cathepsin activity levels are increased, suggesting increased proteolysis through the lysosomal pathway. Together, these mechanisms could supply amino acids as metabolic fuels. In gill, the situation is quite different. It appears that during the first stages of starvation, both protein synthesis and protein degradation are enhanced in gill. This is based upon increased phosphorylation of 4E-BP1 and enhanced levels of UPS indicators, especially 20S proteasome activity and polyubiquitin mRNA. It is proposed that an increased protein turnover is related to gill remodeling perhaps to retain essential hemolymph-borne compounds.
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Auteur Li, X.; Blancheton, J.-P.; Liu, Y.; Triplet, S.; Michaud, L.
Titre Effect of oxidation-reduction potential on performance of European sea bass (Dicentrarchus labrax) in recirculating aquaculture systems Type Article scientifique
Année 2014 Publication (up) Revue Abrégée Aquaculture International
Volume 22 Numéro 4 Pages 1263-1282
Mots-Clés culture-system; disinfection; european sea bass; Orp; ozonated; Ozone; performance; Ras; responses; rock lobster; salinity; seawater; sublethal exposure; trout oncorhynchus-mykiss; water-quality
Résumé The direct impact of oxidation-reduction potential (ORP) on fish welfare and water quality in marine recirculating aquaculture systems (RAS) is poorly documented. In this study, the effects of the fish size (S-1, S-2, S-3) and ORP level (normal, four successive levels) on the performance of European sea bass (Dicentrarchus labrax) were investigated. Three size fish were distributed into two RAS (RAS and RAS O-3). Ozone was injected into RAS O-3 to increase the ORP level. The ORP was stabilized to four successive levels: 260-300, 300-320, 320-350, and 300-320 mV in fish tanks during four periods (P1-4). At the last day of each period, the hematological parameters, plasma protein and mortality of sea bass were analyzed. Two-way ANOVA revealed that several hematological parameters, including pH, hematocrit, concentrations of oxygen, carbon dioxide, glucose (Glu), ionized calcium, kalium, and hemoglobin, were significantly influenced by the increased ORP levels over the experimental period. The alteration in blood Glu and plasma protein concentration showed that ORP around 300-320 mV started to stress sea bass. Once the ORP exceeded 320 mV in the tanks during the P-3 period, mortality occurred even when total residual oxidants/ozone-produced oxidants was only 0.03-0.05 mg L-1 in the fish tanks. At the same time, plasma protein decreased notably due to appetite depression. After the decrease in ORP during the P-4 period, mortality continued. In conclusion, the results strongly suggest that for European sea bass in RAS, the ORP should not exceed 320 mV in the tanks. Once ozonation damaged fish, the effect seemed to be irreversible. However, how ORP affected related hematological parameters still need the further investigations.
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Notes <p>ISI Document Delivery No.: AL4MD<br/>Times Cited: 1<br/>Cited Reference Count: 35<br/>Li, Xian Blancheton, Jean-Paul Liu, Ying Triplet, Sebastien Michaud, Luigi<br/>National Natural Science Foundation of China [41306152]; National Science and Technology Support Program [2011BAD13B04]<br/>The authors would thank all the participants from the Ifremer Palavas station: Cyrille Przybyla, Myriam Callier, and Thibault Geoffroy for their contribution to the experiment and analyses. This work was supported by the National Natural Science Foundation of China (Grant No. 41306152) and National Science and Technology Support Program (Grant No. 2011BAD13B04).<br/>Springer<br/>Dordrecht</p> Approuvé pas de
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Auteur van der Oost, R.; McKenzie, D.J.; Verweij, F.; Satumalay, C.; van der Molen, N.; Winter, M.J.; Chipman, J.K.
Titre Identifying adverse outcome pathways (AOP) for Amsterdam city fish by integrated field monitoring Type Article scientifique
Année 2020 Publication (up) Revue Abrégée Environ. Toxicol. Pharmacol.
Volume 74 Numéro Pages 103301
Mots-Clés Adverse outcome pathways; bioanalytical strategy; Biochemical & physiological biomarkers; biomarker responses; Ecological studies; eel anguilla-anguilla; environmental risk-assessment; Micropollutants risk assessment; oxygen-consumption; rainbow-trout; sole parophrys-vetulus; swimming performance; trout oncorhynchus-mykiss; xenobiotic-metabolizing enzymes
Résumé The European City Fish project aimed to develop a generic methodology for ecological risk assessment for urban rivers. Since traditional methods only consider a small fraction of substances present in the water cycle, biological effect monitoring is required for a more reliable assessment of the pollution status. A major challenge for environmental risk assessment (ERA) is the application of adverse outcome pathways (AOP), i.e. the linking of pollutant exposure via early molecular and biochemical changes to physiological effects and, ultimately, effects on populations and ecosystems. We investigated the linkage between responses at these different levels. Many AOP aspects were investigated, from external and internal exposure to different classes of micropollutants, via molecular key events (MICE) the impacts on organs and organisms (fish physiology), to changes in the population dynamics of fish. Risk assessment procedures were evaluated by comparing environmental quality standards, bioassay responses, biomarkers in caged and feral fish, and the impact on fish populations. Although no complete AOP was observed, indirect relationships linking pollutant exposure via MICE to impaired locomotion were demonstrated at the most polluted site near a landfill for chemical waste. The pathway indicated that several upstream key events requiring energy for stress responses and toxic defence are likely to converge at a single common MKE: increased metabolic demands. Both fish biomarkers and the bioanalytical SIMONI strategy are valuable indicators for micropollutant risks to fish communities.
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ISSN 1382-6689 ISBN Médium
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