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Auteur Fauvel, C.; Boryshpolets, S.; Cosson, J.; Leedy, J.G.W.; Labbe, C.; Haffray, P.; Suquet, M.
Titre Improvement of chilled seabass sperm conservation using a cell culture medium Type Article scientifique
Année 2012 Publication Revue Abrégée J. Appl. Ichthyol.
Volume 28 Numéro 6 Pages 961-966
Mots-Clés dicentrarchus-labrax spermatozoa; fertilizing-capacity; fish; l. spermatozoa; motility; osmolality; psetta-maxima; quality; rainbow-trout; turbot scophthalmus-maximus
Résumé The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4 degrees C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.
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Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0175-8659 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 650
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Auteur Suquet, M.; Cosson, J.; Donval, A.; Labbe, C.; Boulais, M.; Haffray, P.; Bernard, I.; Fauvel, C.
Titre Marathon vs sprint racers: an adaptation of sperm characteristics to the reproductive strategy of Pacific oyster, turbot and seabass Type Article scientifique
Année 2012 Publication Revue Abrégée J. Appl. Ichthyol.
Volume 28 Numéro 6 Pages 956-960
Mots-Clés activation; crassostrea-gigas; fertilization; marine fish; motility; movement; pecten-maximus; respiration; scophthalmus-maximus; spermatozoa
Résumé This work assesses the present knowledge on Pacific oyster sperm biology in comparison to two marine fish species (turbot and seabass) whose sperm characteristics are well described. Sperm morphology mainly differs by the presence of an acrosome in Pacific oyster which is absent in both fish species. In turbot as in Pacific oyster, a sperm maturation process along the genital tract is observed. Sperm motility is triggered by changes in osmolality for seabass and turbot and in pH for Pacific oyster. However, complementary factors are involved to maintain sperm immotile in the genital tract. Sperm movement duration is very long in Pacific oyster (2024 h), compared to turbot (35 min) and seabass (4050 s). A high capacity of ATP regeneration is observed in Pacific oyster sperm, sustained by the limited changes in its morphology observed at the end of the swimming phase. Then, the total distance covered by spermatozoa is very different among the studied species (seabass: 2 mm, turbot: 12 mm, Pacific oyster: 1 m). Considering the main characteristics of sperm movement, the three studied species can be separated in two groups: the sprint racer group (seabass: high velocity and short distance covered) and the marathonian racer one (Pacific oyster: low velocity but covering long distances). To an intermediate extent, turbot sperm belongs to the sprint racer group. Then, the two different sperm movement strategies observed in the three species, are compensated by the behaviour of the breeders.
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Auteur institutionnel Thèse
Editeur Lieu de Publication Éditeur
Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0175-8659 ISBN Médium
Région Expédition Conférence
Notes Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 701
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