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Auteur Pham, D.; Charmantier, G.; Boulo, V.; Wabete, N.; Ansquer, D.; Dauga, C.; Grousset, E.; Labreuche, Y.; Charmantier-Daures, M. url  doi
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  Titre Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae): Deciphering the role of the Na+/K+-ATPase Type Article scientifique
  Année 2016 Publication Revue Abrégée Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology  
  Volume 196–197 Numéro Pages 27-37  
  Mots-Clés Litopenaeus stylirostris; Na+/K+-ATPase; Ontogeny; Osmoregulation; Penaeid; RNA interference  
  Résumé The role of the main ion transporting enzyme Na +/K +-ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the mRNA coding for the α-subunit of Na + K +-ATPase was characterized in L. stylirostris. The resulting 3092-bp cDNA (LsNKA) encodes a putative 1011-amino-acid protein with a predicted molecular mass of 112.3 kDa. The inferred amino acid sequence revealed that the putative protein possesses the main structural characteristics of the Na + K +-ATPase α-subunits. Quantitative RT-PCR analyses indicated that LsNKA transcripts did not significantly vary between the different developmental stages. The number of transcripts was about 2.5-fold higher in the epipodites and gills than in any other tissues tested in juveniles. A reverse genetic approach was finally implemented to study the role of LsNKA in vivo. Knockdown of LsNKA expression by gene-specific dsRNA injection led to an increase of shrimp mortality following an abrupt salinity change compared to control animals. These data strongly suggest that LsNKA plays an important role in osmoregulation when the shrimp are challenged by changing salinities.  
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  Volume de collection Numéro de collection Edition  
  ISSN 1096-4959 ISBN Médium  
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  Numéro d'Appel MARBEC @ alain.herve @ collection 1481  
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Auteur Boudour-Boucheker, N.; Boulo, V.; Charmantier-Daures, M.; Anger, K.; Charmantier, G.; Lorin-Nebel, C. url  doi
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  Titre Osmoregulation in larvae and juveniles of two recently separated Macrobrachium species: Expression patterns of ion transporter genes Type Article scientifique
  Année 2016 Publication Revue Abrégée Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology  
  Volume 195 Numéro Pages 39-45  
  Mots-Clés Branchial chamber; Crustaceans; Na+/K+-ATPase; NHE3 transporter; Salinity; V-type H+-ATPase  
  Résumé In this comparative study, osmoregulatory mechanisms were analyzed in two closely related species of palaemonid shrimp from Brazil, Macrobrachium pantanalense and Macrobrachium amazonicum. A previous investigation showed that all postembryonic stages of M. pantanalense from inland waters of the Pantanal are able to hyper-osmoregulate in fresh water, while this species was not able to hypo-osmoregulate at high salinities. In M. amazonicum originating from the Amazon estuary, in contrast, all stages are able to hypo-osmoregulate, but only first-stage larvae, late juveniles and adults are able to hyper-osmoregulate in fresh water. The underlying molecular mechanisms of these physiological differences have not been known. We therefore investigated the expression patterns of three ion transporters (NKA α-subunit, VHA B-subunit and NHE3) following differential salinity acclimation in different ontogenetic stages (stage-V larvae, juveniles) of both species. Larval NKAα expression was at both salinities significantly higher in M. pantanalense than in M. amazonicum, whereas no difference was noted in juveniles. VHA was also more expressed in larvae of M. pantanalense than in those of M. amazonicum. When NHE3 expression is compared between the larvae of the two species, further salinity-related differences were observed, with generally higher expression in the inland species. Overall, a high expression of ion pumps in M. pantanalense suggests an evolutionary key role of these transporters in freshwater invasion.  
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  Volume de collection Numéro de collection Edition  
  ISSN 1095-6433 ISBN Médium  
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  Numéro d'Appel MARBEC @ alain.herve @ collection 1483  
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Auteur Lorin-Nebel, C.; Felten, V.; Blondeau-Bidet, E.; Grousset, E.; Amilhat, E.; Simon, G.; Biagianti, S.; Charmantier, G. url  doi
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  Titre Individual and combined effects of copper and parasitism on osmoregulation in the European eel Anguilla anguilla Type Article scientifique
  Année 2013 Publication Revue Abrégée Aquatic toxicology  
  Volume 130–131 Numéro Pages 41-50  
  Mots-Clés Anguilla anguilla; Anguillicoloides crassus; Copper; Gills; Ionic concentrations; Na+/K+-ATPase activity; osmolality  
  Résumé The European eel (Anguilla anguilla), a catadromous species, breeds in the sea and migrates to estuarine, lagoon or freshwater habitats for growth and development. Yellow eels, exposed to low or fluctuating salinities, are also exposed to multiple other stressors as pollution, over-fishing and parasitism, which contribute to the dramatic decrease of eel populations in several European countries. The objective of this study was to evaluate the single and combined effects of waterborne copper and experimental infestation of eels with the nematode Anguillicoloides crassus after a salinity challenge from nearly isotonic (18ppt) to hypo- (5ppt) and hypertonic (29ppt) conditions, in order to investigate the osmoregulatory capacity of eels exposed to these stressors. In a nearly isotonic condition (18ppt), blood osmolality remained constant over the 6 weeks contamination to Cu(2+) and Anguillicoloides crassus. In fish exposed to a salinity challenge of 29ppt for 2 weeks, no significant effect was recorded in blood osmolality, Na(+)/K(+)-ATPase (NKA) activity, Na(+) and Cl(-) concentrations. After 2 weeks at 5ppt however, a significant blood osmolality decrease was detected in fish exposed to Anguillicoloides crassus infestation with or without Cu(2+) addition. This decrease may originate from lower Cl(-) levels measured in eels exposed to both stressors. Blood Na(+) levels remained relatively stable in all tested animals, but gill NKA activities were lower in eels exposed to combined stress. No apparent branchial lesions were detected following the different treatments and immunolocalization of NKA revealed well-differentiated ionocytes. Thus, the 5ppt challenge in eels exposed to copper and Anguillicoloides crassus seems to clearly enhance iono/osmoregulatory disturbances. Funded by ANR CES/CIEL 2008-12.  
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  Langue Langue du Résumé Titre Original  
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  Volume de collection Numéro de collection Edition  
  ISSN 0166-445x ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 686  
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Auteur Dayras, P.; Charmantier, G.; Chaumot, A.; Vigneron, A.; Coquery, M.; Quéau, H.; Artells, E.; Lignot, J.-H.; Geffard, O.; Issartel, J. url  doi
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  Titre Osmoregulatory responses to cadmium in reference and historically metal contaminated Gammarus fossarum (Crustacea, Amphipoda) populations Type Article scientifique
  Année 2017 Publication Revue Abrégée Chemosphere  
  Volume 180 Numéro Pages 412-422  
  Mots-Clés Cadmium; Crustacean; Histology; immunolocalization; Na+/K+-ATPase; V-H+-ATPase  
  Résumé In order to better understand the variable sensitivities of crustaceans to metals, we investigated the impact of cadmium exposure in 3 populations of Gammarus fossarum from different rivers of France. The first population lives in a Cd-contaminated river from a geochemical background, while the others inhabit Cd-free sites. Osmoregulation, a relevant biomarker to evaluate crustacean health following metal contamination, was used as a proxy to evaluate the intra- and inter-populationnal sensitivities to Cd. Specimens from each population were experimentally exposed to 9 μg Cd2+/L Cd for 7 days and hemolymph osmolality (HO) was then individually measured. In exposed populations, high inter-individual variations in HO values were noted, resulting in their separation into non-impacted and slightly or highly Cd-impacted (with lower HO) animals. In gills of impacted organisms, deep histopathological alterations and protein overexpression of Na+/K+-ATPase and V-H+-ATPase were observed through histology and immunolocalization, while non-impacted animals showed profiles comparable to controls. Moreover, the osmoregulatory processes in the population living in the Cd-contaminated site were impacted by acute Cd exposure in the laboratory as much as for one of the two populations originating from Cd-free sites. The observed changes did not reveal any obvious adaptive osmoregulatory phenomena at the population scale, but they may be due to differences in fitness between individuals and between populations in relation to the features of their respective environments, unrelated with the presence of the metal.  
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  Langue Langue du Résumé Titre Original  
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  Volume de collection Numéro de collection Edition  
  ISSN 0045-6535 ISBN Médium  
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  Numéro d'Appel MARBEC @ alain.herve @ collection 2104  
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Auteur Boudour-Boucheker, N.; Boulo, V.; Lorin-Nebel, C.; Elguero, C.; Grousset, E.; Anger, K.; Charmantier-Daures, M.; Charmantier, G. url  doi
openurl 
  Titre Adaptation to freshwater in the palaemonid shrimp Macrobrachium amazonicum: comparative ontogeny of osmoregulatory organs Type Article scientifique
  Année 2013 Publication Revue Abrégée Cell Tissue Res  
  Volume 353 Numéro 1 Pages 87-98  
  Mots-Clés Antennal gland; Branchial chamber; Crustaceans; Immunolocalization; Macrobrachium amazonicum; Na+/K+-ATPase; Osmoregulation  
  Résumé  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0302-766x ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 441  
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