bascule de visibilité Search & Display Options

Tout Sélectionner    Désélectionner
 |   | 
Détails
   print
  Enregistrements Liens
Auteur (up) Pham, D.; Charmantier, G.; Boulo, V.; Wabete, N.; Ansquer, D.; Dauga, C.; Grousset, E.; Labreuche, Y.; Charmantier-Daures, M. url  doi
openurl 
  Titre Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae): Deciphering the role of the Na+/K+-ATPase Type Article scientifique
  Année 2016 Publication Revue Abrégée Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology  
  Volume 196–197 Numéro Pages 27-37  
  Mots-Clés Litopenaeus stylirostris; Na+/K+-ATPase; Ontogeny; Osmoregulation; Penaeid; RNA interference  
  Résumé The role of the main ion transporting enzyme Na +/K +-ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the mRNA coding for the α-subunit of Na + K +-ATPase was characterized in L. stylirostris. The resulting 3092-bp cDNA (LsNKA) encodes a putative 1011-amino-acid protein with a predicted molecular mass of 112.3 kDa. The inferred amino acid sequence revealed that the putative protein possesses the main structural characteristics of the Na + K +-ATPase α-subunits. Quantitative RT-PCR analyses indicated that LsNKA transcripts did not significantly vary between the different developmental stages. The number of transcripts was about 2.5-fold higher in the epipodites and gills than in any other tissues tested in juveniles. A reverse genetic approach was finally implemented to study the role of LsNKA in vivo. Knockdown of LsNKA expression by gene-specific dsRNA injection led to an increase of shrimp mortality following an abrupt salinity change compared to control animals. These data strongly suggest that LsNKA plays an important role in osmoregulation when the shrimp are challenged by changing salinities.  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 1096-4959 ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 1481  
Lien permanent pour cet enregistrement
 

 
Auteur (up) Rind, K.; Rodriguez-Barucg, Q.; Nicolas, D.; Cucchi, P.; Lignot, J.-H. doi  openurl
  Titre Morphological and physiological traits of Mediterranean sticklebacks living in the Camargue wetland (Rhone river delta) Type Article scientifique
  Année 2020 Publication Revue Abrégée J. Fish Biol.  
  Volume Numéro Pages  
  Mots-Clés 3-spined stickleback; ecological causes; Gasterosteus aculeatus; gasterosteus-aculeatus l; gill rakers; ion regulation; k+-atpase activity; morphometry; na+/k+-atpase; NKA activity; osmoregulation; oxygen consumption; oxygen-consumption; pelvic reduction; plate morph evolution; salinity; stickleback; threespine stickleback  
  Résumé Three-spined sticklebacks (Gasterosteus aculeatus L.) living at the southern limit of the species distribution range could possess specific morphological and physiological traits that enable these fish to live at the threshold of their physiological capacities. Morphological analysis was carried out on samples of sticklebacks living in different saline habitats of the Camargue area (Rhone delta, northern Mediterranean coast) obtained from 1993 to 2017. Salinity acclimation capacities were also investigated using individuals from freshwater-low salinity drainage canals and from mesohaline-euryhaline lagoons. Fish were maintained in laboratory conditions at salinity values close to those of their respective habitats: low salinity (LS, 5 parts per thousand) or seawater (SW, 30 parts per thousand). Fish obtained from a mesohaline brackish water lagoon (BW, 15 parts per thousand) were acclimated to SW or LS. Oxygen consumption rates and branchial Na+/K+-ATPase (NKA) activity (indicator of fish osmoregulatory capacity) were measured in these LS or SW control fish and in individuals subjected to abrupt SW or LS transfers. At all the studied locations, only the low-plated “leiurus” morphotype showed no spatial or temporal variations in their body morphology. Gill rakers were only longer and denser in fish sampled from the LS-freshwater (FW) drainage canals. All fish presented similar physiological capacities. Oxygen consumption rates were not influenced by salinity challenge except in SW fish transferred to LS immediately and 1 h after transfer. However, and as expected, gill NKA activity was salinity dependent. Sticklebacks of the Camargue area sampled from habitats with contrasted saline conditions are homogenously euryhaline, have low oxygen consumption rates and do not appear to experience significantly greater metabolic costs when challenged with salinity. However, an observed difference in gill raker length and density is most probably related to the nutritional condition of their habitat, indicating that individuals can rapidly acclimatize to different diets.  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0022-1112 ISBN Médium  
  Région Expédition Conférence  
  Notes WOS:000526005600001 Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2770  
Lien permanent pour cet enregistrement
 

 
Auteur (up) Riou, V.; Ndiaye, A.; Budzinski, H.; Dugue, R.; Le Menach, K.; Combes, Y.; Bossus, M.; Durand, J.D.; Charmantier, G.; Lorin-Nebel, C. url  doi
openurl 
  Titre Impact of environmental DDT concentrations on gill adaptation to increased salinity in the tilapia Sarotherodon melanotheron Type Article scientifique
  Année 2012 Publication Revue Abrégée Comp. Biochem. Physiol. C-Toxicol. Pharmacol.  
  Volume 156 Numéro 1 Pages 7-16  
  Mots-Clés DDTs; Na+/K+-ATPase; Osmoregulation; Rainbow trout; Teleost; cell responses; crucian carp; fish; gill; goldfish carassius-auratus; na-k-atpase; natural-populations; polychlorinated-biphenyls; salmo-gairdneri; water  
  Résumé Estuaries of tropical developing countries suffering from severe droughts induced by climate change are habitats to fish, which face drastic salinity variations and the contact with pollutants. The Western Africa tilapia Sarotherodon melanotheron is highly resistant to hypersalinity, but the effect of human-released xenobiotics on its adaptation is barely known. Controlled experiments were conducted to observe S. melanotheron gill adaptation to abrupt salinity variations in the presence of waterborne DDT, at concentrations detected in their natural habitat. The gills appeared as an important site of DDT conversion to DDD and/or depuration. A 12-days DDT exposure resulted in decreased gill epithelium thickness at all salinities (from fresh- to hypersaline-water), and the structure of gills from freshwater fish was particularly altered, relative to controls. No unbalance in tilapia blood osmolality was observed following DDT exposure, which however caused a decrease in branchial Na+-K+-ATPase (NKA) activity. Gill cellular NKA expression was reduced in salt-water, together with the expression of the CFTR chloride channel in hypersaline water. Although S. melanotheron seems very resistant (especially in seawater) to short-term waterborne DDT contamination, the resulting alterations of the gill tissue, cells and enzymes might affect longer term respiration, toxicant depuration and/or osmoregulation in highly fluctuating salinities. (C) 2012 Elsevier Inc. All rights reserved.  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 1532-0456 ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 649  
Lien permanent pour cet enregistrement
 

 
Auteur (up) Rivera-Ingraham, G.A.; Barri, K.; Boel, M.; Farcy, E.; Charles, A.-L.; Geny, B.; Lignot, J.-H. doi  openurl
  Titre Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function? Type Article scientifique
  Année 2016 Publication Revue Abrégée J. Exp. Biol.  
  Volume 219 Numéro 1 Pages 80-89  
  Mots-Clés Antioxidant defense; antioxidant enzymes; blue-crab; callinectes-sapidus; Carcinus aestuarii; crab carcinus-maenas; green crab; na+/k+-atpase activity; Osmoregulation; progressive hypoxia; reactive oxygen; ROS production; shore crab; temporal distribution  
  Résumé Osmoregulating decapods such as the Mediterranean green crab Carcinus aestuarii possess two groups of spatially segregated gills: anterior gills serve mainly respiratory purposes, while posterior gills contain osmoregulatory structures. The co-existence of similar tissues serving different functions allows the study of differential adaptation, in terms of free radical metabolism, upon salinity change. Crabs were immersed for 2 weeks in seawater (SW, 37 ppt), diluted SW (dSW, 10 ppt) and concentrated SW (cSW, 45 ppt). Exposure to dSW was the most challenging condition, elevating respiration rates of whole animals and free radical formation in hemolymph (assessed fluorometrically using C-H(2)DFFDA). Further analyses considered anterior and posterior gills separately, and the results showed that posterior gills are the main tissues fueling osmoregulatory-related processes because their respiration rates in dSW were 3.2-fold higher than those of anterior gills, and this was accompanied by an increase in mitochondrial density (citrate synthase activity) and increased levels of reactive oxygen species (ROS) formation (1.4-fold greater, measured through electron paramagnetic resonance). Paradoxically, these posterior gills showed undisturbed caspase 3/7 activity, used here as a marker for apoptosis. This may only be due to the high antioxidant protection that posterior gills benefit from [superoxide dismutase (SOD) in posterior gills was over 6 times higher than in anterior gills]. In conclusion, osmoregulating posterior gills are better adapted to dSW exposure than respiratory anterior gills because they are capable of controlling the deleterious effects of the ROS production resulting from this salinity-induced stress.  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0022-0949 ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 1541  
Lien permanent pour cet enregistrement
Tout Sélectionner    Désélectionner
 |   | 
Détails
   print

Save Citations:
Export Records: