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Auteur Shirangi, S.A.; Kalbassi, M.R.; Khodabandeh, S.; Jafarian, H.; Lignot, J.-H. doi  openurl
  Titre Immunolocalization of Na+/K+-ATPase, Na+/K+/2Cl- co-transporter (NKCC) and mRNA expression of Na+/K+-ATPase +α-subunit during short-term salinity transfer in the gills of Persian sturgeon (Acipenser persicus, Borodin, 1897) juveniles Type Article scientifique
  Année 2017 Publication (up) Revue Abrégée J. Appl. Ichthyol.  
  Volume 33 Numéro 3 Pages 347-353  
  Mots-Clés acclimation; bass dicentrarchus-labrax; cftr anion channel; fish; gene-expression; na+,k+,2cl(-) cotransporter; na+,k+-atpase; Osmoregulation; rich cells; salmon salmo-salar  
  Résumé The study tests the physiological responses of Persian sturgeon, Acipenser persicus, during the abrupt release of juveniles from freshwater (FW) into brackish waters (BW=11 parts per thousand) of the Caspian Sea. Fish weight at release was 2-3g (2.55 +/- 0.41g; 8.8 +/- 0.58cm TL). Totals of 160 individuals were randomly distributed into four fiber-glass aerated tanks (volume 60-L). Two tanks served as controls (FW groups), and two as exposure tanks for BW (Caspian Sea water=CSW). Fish were sampled at 0, 3, 6, 12, 24, 48 and 96hr after abrupt transfer to CSW. Plasma osmolality, immunolocalization of Na+, K+ -ATPase (NKA) and Na+/K+/2Cl(-) (NKCC) Co-transporter, NKA activity and the NKA -subunit mRNA expression were analyzed. Blood osmolality of fish transferred from FW to CSW increased significantly within hours post-transfer (p<.05) and remained at a high level for up to 96hr. Immunolocalization of NKCC indicated co-localization with NKA in the chloride cells in the gill epithelium. A partial sequence of the NKA -subunit (632bp) is described. Its expression levels were up-regulated at 12 and 48hr following salinity transfer (p<.05). However, NKA activity sharply increased in CSW specimens by almost 2.8-fold (p<.05) between 48 and 96hr after transfer. Gill NKCC co-transporter abundance increased, coinciding with increased gill NKA activity. The increased activity of NKCC during salt excretion in CSW may lead to an influx of Na+ into the chloride cells. Consequently, NKA activity increases to maintain intracellular Na+ homeostasis.  
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  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0175-8659 ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 2152  
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Auteur Sellami, I.; Charmantier, G.; Naceur, H.B.; Kacem, A.; Lorin-Nebel, C. doi  openurl
  Titre Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia) Type Article scientifique
  Année 2020 Publication (up) Revue Abrégée Tissue Cell  
  Volume 63 Numéro Pages Unsp-101340  
  Mots-Clés alpha-subunit; Artemia salina; brine shrimp artemia; fresh-water; homarus-gammarus crustacea; Hypo-osmoregulation; Immunolocalization; ionic regulation; Maxillary glands; Metepipodites; na+,k+-atpase; Na+/K+ -ATPase; ontogeny; osmotic regulation; potential difference; salt-gland  
  Résumé Artemia salina is an extremophile species that tolerates a wide range of salinity, especially hypertonic media considered lethal for the majority of other aquatic species. In this study, A. salina cysts were hatched in the laboratory and nauplii were acclimated at three different salinities (60, 139 and 212 ppt). Once in the adult phase, their hemolymph osmolality was measured. The animals were strong hypo-osmoregulators in the entire range of tested salinities, with up to 10 fold lower hemolymph osmolalities than their surrounding environment. Immunostaining of Na+/K+-ATPase was done on sections and on whole body mounts of adults in order to localize the ionocytes in different organs. An intense Na+/K+-ATPase immunostaining throughout the cells was observed in the epithelium of the ten pairs of metepipodites. A positive immunoreactivity for Na+/K+-ATPase was also detected in the maxillary glands, in the epithelium of the efferent tubule and of the excretory canal, as well as in the anterior digestive tract. This study confirms the strong hypo-osmotic capacity of this species and affords an overview of the different organs involved in osmoregulation in A. salina adults.  
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  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0040-8166 ISBN Médium  
  Région Expédition Conférence  
  Notes WOS:000522853900012 Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2758  
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