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Auteur (down) Xiong, W.; Gao, S.; Lu, Y.; Wei, L.; Mao, J.; Xie, J.; Cao, Q.; Liu, J.; Bi, J.; Song, X.; Li, B.
Titre Latrophilin participates in insecticide susceptibility through positively regulating CSP10 and partially compensated by OBPC01 in Tribolium castaneum Type Article scientifique
Année 2019 Publication Revue Abrégée Pest. Biochem. Physiol.
Volume 159 Numéro Pages 107-117
Mots-Clés Carbofuran; chemosensory proteins; detoxification; Dichlorvos; evolution; expression; family; gene; identification; Insecticide susceptibility; Latrophilin; odorant-binding proteins; red flour beetle; rna interference; RNA interference; Tribolium castaneum
Résumé Latrophilin (LPH) is an adhesion G protein-coupled receptor (aGPCR) that participates in multiple essential physiological processes. Our previous studies have shown that lph is not only indispensable for the development and reproduction of red flour beetles (Tribolium castaneum), but also for their resistance against dichlorvos or carbofuran insecticides. However, the regulatory mechanism of lph-mediated insecticide susceptibility remains unclear. Here, we revealed that knockdown of lph in beetles resulted in opposing changes in two chemoreception genes, chemosensory protein 10 (CSP10) and odorant-binding protein C01 (OBPC01), in which the expression of TcCSP10 was downregulated, whereas the expression of TcOBPC01 was upregulated. TcCSP10 and TcOBPC01 were expressed at the highest levels in early pupal and late larval stages, respectively. High levels of expression of both these genes were observed in the heads (without antennae) of adults. TcCSP10 and TcOBPC01 were significantly induced by dichlorvos or carbofuran between 12 and 72 h (hrs) after exposure, suggesting that they are likely associated with increasing the binding affinity of insecticides, leading to a decrease in sensitivity to the insecticides. Moreover, once these two genes were knocked down, the susceptibility of the beetles to dichlorvos or carbofuran was enhanced. Additionally, RNA interference (RNAi) targeting of lph followed by exposure to dichlorvos or carbofuran also caused the opposing expression levels of TcCSP10 and TcOBPC01 compared to the expression levels of wild-type larvae treated with insecticides alone. All these results indicate that lph is involved in insecticide susceptibility through positively regulating TcCSP10; and the susceptibility could also further partially compensated for through the negative regulation of TcOBPC01 when lph was knockdown in the red flour beetle. Our studies shed new light on the molecular regulatory mechanisms of lph related to insecticide susceptibility.
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Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0048-3575 ISBN Médium
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Notes WOS:000482696800015 Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2645
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Auteur (down) Villon, S.; Mouillot, D.; Chaumont, M.; Darling, E.S.; Subsol, G.; Claverie, T.; Villeger, S.
Titre A Deep learning method for accurate and fast identification of coral reef fishes in underwater images Type Article scientifique
Année 2018 Publication Revue Abrégée Ecol. Inform.
Volume 48 Numéro Pages 238-244
Mots-Clés Automated identification; Convolutional neural network; density; Machine learning; Marine fishes; neural-networks; system; temperate; Underwater pictures; video stations; visual census; vulnerability
Résumé Identifying and counting fish individuals on photos and videos is a crucial task to cost-effectively monitor marine biodiversity, yet it remains difficult and time-consuming. In this paper, we present a method to assist the identification of fish species on underwater images, and we compare our model performances to human ability in terms of speed and accuracy. We first tested the performance of a convolutional neural network (CNN) trained with different photographic databases while accounting for different post-processing decision rules to identify 20 fish species. Finally, we compared the performance of species identification of our best CNN model with that of humans on a test database of 1197 fish images representing nine species. The best CNN was the one trained with 900,000 images including (i) whole fish bodies, (ii) partial fish bodies and (iii) the environment (e.g. reef bottom or water). The rate of correct identification was 94.9%, greater than the rate of correct identification by humans (89.3%). The CNN was also able to identify fish individuals partially hidden behind corals or behind other fish and was more effective than humans to identify fish on smallest or blurry images while humans were better to identify fish individuals in unusual positions (e.g. twisted body). On average, each identification by our best CNN using a common hardware took 0.06 s. Deep Learning methods can thus perform efficient fish identification on underwater images and offer promises to build-up new video-based protocols for monitoring fish biodiversity cheaply and effectively.
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Langue English Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 1574-9541 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2475
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Auteur (down) Santana, F.M.; Morize, E.; Labonne, M.; Lessa, R.; Clavier, J.
Titre Connectivity between the marine coast and estuary for white mullet (Mugil curema) in northeastern Brazil revealed by otolith Sr:Ca ratio Type Article scientifique
Année 2018 Publication Revue Abrégée Estuar. Coast. Shelf Sci.
Volume 215 Numéro Pages 124-131
Mots-Clés anguilla-japonica; Brackish water; ca ratios; calcium ratios; cephalus; identification; japanese eel; Migrations; migratory environmental history; Mugil curema; Otoliths; patterns; salinity; Sea water; strontium incorporation; White mullet
Résumé Microchemical analyses were carried out in order to estimate the Strontium:Calcium (Sr:Ca) ratio in the otolith of the white mullet, Mugil curema, in the Pernambuco (at the Santa Cruz channel, Brazil) in order to determine its connectivity between the estuary and ocean. Variation in the otolith Sr:Ca ratio was directly related to salinity, with greater salinity denoting a higher otolith Sr:Ca value. Data on the otolith Sr:Ca ratio demonstrates that the individuals analyzed are born in areas of salinity that are characteristic of the estuary, where they develop until approximately one year of age, at which point they migrate to areas of greater salinity until reaching sexual maturity (3 years of age) in the sea. Spawning occurs in the ocean, after which M. curema individuals may either remain or return to the estuary until the next spawning. Differences in estuarine salinity were found for young of-year individuals and may be related to the season when spawning took place, since M. curema females are found spawning throughout the year. The hypothesis is that higher salinity in the dry season leads to a greater otolith Sr.Ca signature among individuals spawned in this season from birth until one year of life. On the other hand, the lower salinity in the rainy season leads to a lower otolith Sr:Ca signature among individuals spawned in this season. These information are important for the adequate management of the white mullet stock in northeastern Brazil.
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Langue English Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 0272-7714 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2474
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Auteur (down) Restrepo-Ortiz, C.X.; Merbt, S.N.; Barrero-Canossa, J.; Fuchs, B.M.; Casamayor, E.O.
Titre Development of a 16S rRNA-targeted fluorescence in situ hybridization probe for quantification of the ammonia-oxidizer Nitrosotalea devanaterra and its relatives Type Article scientifique
Année 2018 Publication Revue Abrégée Syst. Appl. Microbiol.
Volume 41 Numéro 4 Pages 408-413
Mots-Clés Ammonia oxidizing; archaea; bacteria; card-fish; catalyzed reporter deposition; cells; crenarchaeota; environments; high-mountain lakes; identification; Nitrosotalea devanaterra; oligonucleotide probes; SAGMCG-1 Group; Soil; thaumarchaeota; Thaumarchaeota
Résumé The Thaumarchaeota SAGMCG-1 group and, in particular, members of the genus Nitrosotalea have high occurrence in acidic soils, the rhizosphere, groundwater and oligotrophic lakes, and play a potential role in nitrogen cycling. In this study, the specific oligonucleotide fluorescence in situ hybridization probe SAG357 was designed for this Thaumarchaeota group based on the available 16S rRNA gene sequences in databases, and included the ammonia-oxidizing species Nitrosotalea devanaterra. Cell permeabilization for catalyzed reporter deposition fluorescence in situ detection and the hybridization conditions were optimized on enrichment cultures of the target species N. devanaterra, as well as the non-target ammonia-oxidizing archaeon Nitrosopumilus maritimus. Probe specificity was improved with a competitor oligonucleotide, and fluorescence intensity and cell visualization were enhanced by the design and application of two adjacent helpers. Probe performance was tested in soil samples along a pH gradient, and counting results matched the expected in situ distributions. Probe SAG357 and the CARD-FISH protocol developed in the present study will help to improve the current understanding of the ecology and physiology of N. devanaterra and its relatives in natural environments. (C) 2018 Elsevier GmbH. All rights reserved.
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Langue English Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 0723-2020 ISBN Médium
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Numéro d'Appel MARBEC @ alain.herve @ collection 2393
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Auteur (down) MAHE, K.; VILLANUEVA, C.-M.; VAZ, S.; COPPIN, F.; KOUBBI, P.; CARPENTIER, A.
Titre Morphological variability of the shape of striped red mullet Mullus surmuletus in relation to stock discrimination between the Bay of Biscay and the eastern English Channel Type Article scientifique
Année 2014 Publication Revue Abrégée Journal Of Fish Biology
Volume 84 Numéro 4 Pages 1063-1073
Mots-Clés fish body shape; head morphology; morphometric analysis; stock identification; Truss model
Résumé Striped red mullet Mullus surmuletus L. 1758 is an economically important species in the Mediterranean Sea and in the northern Atlantic Ocean, where it is exploited from the Bay of Biscay to the southern North Sea (ICES, 2010). In Atlantic waters, there are two main areas where this species is caught, the Bay of Biscay and the eastern English Channel. This species was initially exploited by the Spanish fleets along the Spanish coast inside the Bay of Biscay. Originally considered as valuable by-catch (Marchal, 2008), the growing exploitation ofM. surmuletus and a conspicuous increase in landings in the English Channel and the southern North Sea by French, English and Dutch fleets have been observed from the 1990s onwards. This was attributed to an increase in the migratory distribution and abundance of this species in these areas, which is largely heightened by the decline of traditionally targeted species and the trend for sea water warming (Poulard&Blanchard, 2005; Marchal, 2008; ICES, 2010).
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Volume de collection Numéro de collection Edition
ISSN 0022-1112 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 1137
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