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Auteur (down) Cormier, B.; Batel, A.; Cachot, J.; Begout, M.-L.; Braunbeck, T.; Cousin, X.; Keiter, S.H. doi  openurl
  Titre Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio) Type Article scientifique
  Année 2019 Publication Revue Abrégée Front. Environ. Sci.  
  Volume 7 Numéro Pages 135  
  Mots-Clés aromatic-hydrocarbons pahs; benzo[a]pyrene; chronic dietary exposure; cyp1a; erod; fish embryotoxicity test (FET); fresh-water; gene-expression; hydrophobic organic-chemicals; marine-environment; oxybenzone; perfluorooctane sulfonate; plastic debris; resin pellets; risk-assessment; swimming behavior; uv-filters  
  Résumé As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a] pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP, and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63).  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2646  
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Auteur (down) Compte-Port, S.; Borrego, C.M.; Moussard, H.; Jeanbille, M.; Restrepo-Ortiz, C.X.; de Diego, A.; Rodriguez-Iruretagoiena, A.; Gredilla, A.; Fdez-Ortiz de Vallejuelo, S.; Galand, P.E.; Kalenitchenko, D.; Rols, J.-L.; Pokrovsky, O.S.; Gonzalez, A.G.; Camarero, L.; Muniz, S.; Navarro-Navarro, E.; Auguet, J.-C. doi  openurl
  Titre Metal contaminations impact archaeal community composition, abundance and function in remote alpine lakes Type Article scientifique
  Année 2018 Publication Revue Abrégée Environ. Microbiol.  
  Volume 20 Numéro 7 Pages 2422-2437  
  Mots-Clés ammonia-oxidizing archaea; fresh-water lakes; high-altitude lakes; high-mountain lakes; mcra gene; methanogenic archaea; microbial communities; ribosomal-rna; tibetan plateau; trace-metals  
  Résumé Using the 16S rRNA and mcrA genes, we investigated the composition, abundance and activity of sediment archaeal communities within 18 high-mountain lakes under contrasted metal levels from different origins (bedrock erosion, past-mining activities and atmospheric depositions). Bathyarchaeota, Euryarchaeota and Woesearchaeota were the major phyla found at the meta-community scale, representing 48%, 18.3% and 15.2% of the archaeal community respectively. Metals were equally important as physicochemical variables in explaining the assemblage of archaeal communities and their abundance. Methanogenesis appeared as a process of central importance in the carbon cycle within sediments of alpine lakes as indicated by the absolute abundance of methanogen 16S rRNA and mcrA gene transcripts (10(5) to 10(9) copies g(-1)). We showed that methanogen abundance and activity were significantly reduced with increasing concentrations of Pb and Cd, two indicators of airborne metal contaminations. Considering the ecological importance of methanogenesis in sediment habitats, these metal contaminations may have system wide implications even in remote area such as alpine lakes. Overall, this work was pioneer in integrating the effect of long-range atmospheric depositions on archaeal communities and indicated that metal contamination might significantly compromise the contribution of Archaea to the carbon cycling of the mountain lake sediments.  
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  ISSN 1462-2912 ISBN Médium  
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  Numéro d'Appel MARBEC @ alain.herve @ collection 2420  
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Auteur (down) Buisson, L.; Grenouillet, G.; Villeger, S.; Canal, J.; Laffaille, P. url  doi
openurl 
  Titre Toward a loss of functional diversity in stream fish assemblages under climate change Type Article scientifique
  Année 2013 Publication Revue Abrégée Glob. Change Biol.  
  Volume 19 Numéro 2 Pages 387-400  
  Mots-Clés assemblages; biotic homogenization; bird communities; climate change; distribution models; ecosystem; environmental-change; fresh-water biodiversity; functional traits; habitat; no-analog communities; range shifts; species; species distribution; stream fish; traits  
  Résumé The assessment of climate change impacts on biodiversity has so far been biased toward the taxonomic identification of the species likely either to benefit from climate modifications or to experience overall declines. There have still been few studies intended to correlate the characteristics of species to their sensitivity to climate change, even though it is now recognized that functional trait-based approaches are promising tools for addressing challenges related to global changes. In this study, two functional indices (originality and uniqueness) were first measured for 35 fish species occurring in French streams. They were then combined to projections of range shifts in response to climate change derived from species distribution models. We set out to investigate: (1) the relationship between the degrees of originality and uniqueness of fish species, and their projected response to future climate change; and (2) the consequences of individual responses of species for the functional diversity of fish assemblages. After accounting for phylogenetic relatedness among species, we have demonstrated that the two indices used measure two complementary facets of the position of fish species in a functional space. We have also rejected the hypothesis that the most original and/or less redundant species would necessarily experience the greatest declines in habitat suitability as a result of climate change. However, individual species range shifts could lead simultaneously both to a severe decline in the functional diversity of fish assemblages, and to an increase in the functional similarity among assemblages, supporting the hypothesis that disturbance favors communities with combination of common traits and biotic homogenization as well. Our findings therefore emphasize the importance of going beyond the simple taxonomic description of diversity to provide a better assessment of the likely future effects of environmental changes on biodiversity, thus helping to design more effective conservation and management measures.  
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  ISSN 1354-1013 ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 897  
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Auteur (down) Bouvier, T.; Venail, P.; Pommier, T.; Bouvier, C.; Barbera, C.; Mouquet, N. url  doi
openurl 
  Titre Contrasted Effects of Diversity and Immigration on Ecological Insurance in Marine Bacterioplankton Communities Type Article scientifique
  Année 2012 Publication Revue Abrégée PLoS One  
  Volume 7 Numéro 6 Pages  
  Mots-Clés 16s ribosomal-rna; bacterial communities; biodiversity; dispersal; fresh-water; gradient gel-electrophoresis; metacommunities; predictability; productivity; stability  
  Résumé The ecological insurance hypothesis predicts a positive effect of species richness on ecosystem functioning in a variable environment. This effect stems from temporal and spatial complementarity among species within metacommunities coupled with optimal levels of dispersal. Despite its importance in the context of global change by human activities, empirical evidence for ecological insurance remains scarce and controversial. Here we use natural aquatic bacterial communities to explore some of the predictions of the spatial and temporal aspects of the ecological insurance hypothesis. Addressing ecological insurance with bacterioplankton is of strong relevance given their central role in fundamental ecosystem processes. Our experimental set up consisted of water and bacterioplankton communities from two contrasting coastal lagoons. In order to mimic environmental fluctuations, the bacterioplankton community from one lagoon was successively transferred between tanks containing water from each of the two lagoons. We manipulated initial bacterial diversity for experimental communities and immigration during the experiment. We found that the abundance and production of bacterioplankton communities was higher and more stable (lower temporal variance) for treatments with high initial bacterial diversity. Immigration was only marginally beneficial to bacterial communities, probably because microbial communities operate at different time scales compared to the frequency of perturbation selected in this study, and of their intrinsic high physiologic plasticity. Such local “physiological insurance” may have a strong significance for the maintenance of bacterial abundance and production in the face of environmental perturbations.  
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  ISSN 1932-6203 ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 499  
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Auteur (down) Boudour-Boucheker, N.; Boulo, V.; Charmantier-Daures, M.; Grousset, E.; Anger, K.; Charmantier, G.; Lorin-Nebel, C. url  doi
openurl 
  Titre Differential distribution of V-type H+-ATPase and Na+/K+-ATPase in the branchial chamber of the palaemonid shrimp Macrobrachium amazonicum Type Article scientifique
  Année 2014 Publication Revue Abrégée Cell and Tissue Research  
  Volume 357 Numéro 1 Pages 195-206  
  Mots-Clés Branchiostegite; Gills; Na+/K+-ATPase; Osmoregulation; V-type H+-ATPase; crab eriocheir-sinensis; decapoda; epithelial potential difference; fresh-water crab; gill epithelium; homarus-gammarus; ion-transport; larval development; lobster; olfersii; plasma-membrane; salinity acclimation  
  Résumé V-H+-ATPase and Na+/K+-ATPase were localized in the gills and branchiostegites of M. amazonicum and the effects of salinity on the branchial chamber ultrastructure and on the localization of transporters were investigated. Gills present septal and pillar cells. In freshwater (FW), the apical surface of pillar cells is amplified by extensive evaginations associated with mitochondria. V-H+-ATPase immunofluorescence was localized in the membranes of the apical evaginations and in clustered subapical areas of pillar cells, suggesting labeling of intracellular vesicle membranes. Na+/K+-ATPase labeling was restricted to the septal cells. No difference in immunostaining was recorded for both proteins according to salinity (FW vs. 25 PSU). In the branchiostegite, both V-H+-ATPase and Na+/K+-ATPase immunofluorescence were localized in the same cells of the internal epithelium. Immunogold revealed that V-H+-ATPase was localized in apical evaginations and in electron-dense areas throughout the inner epithelium, while Na+/K+-ATPase occurred densely along the basal infoldings of the cytoplasmic membrane. Our results suggest that morphologically different cell types within the gill lamellae may also be functionally specialized. We propose that, in FW, pillar cells expressing V-H+-ATPase absorb ions (Cl-, Na+) that are transported either directly to the hemolymph space or through a junctional complex to the septal cells, which may be responsible for active Na+ delivery to the hemolymph through Na+/K+-ATPase. This suggests a functional link between septal and pillar cells in osmoregulation. When shrimps are transferred to FW, gill and branchiostegite epithelia undergo ultrastructural changes, most probably resulting from their involvement in osmoregulatory processes.  
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  ISSN 0302-766x ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 541  
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