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Auteur (down) Sucre, E.; Charmantier-Daures, M.; Grousset, E.; Charmantier, G.; Cucchi-Mouillot, P.
Titre Early development of the digestive tract (pharynx and gut) in the embryos and pre-larvae of the European sea bass Dicentrarchus labrax Type Article scientifique
Année 2009 Publication Revue Abrégée J. Fish Biol.
Volume 75 Numéro 6 Pages 1302-1322
Mots-Clés Moronidae; arch; atlantic salmon; chloride cells; development; embryo; gill arches; gill slits; histology; medaka oryzias-latipes; ontogeny; oreochromis-niloticus; paralichthys-olivaceus embryos; rainbow-trout; respiratory development; urinary system; zebrafish
Résumé The European sea bass Dicentrarchus labrax is a marine teleost important in Mediterranean aquaculture. The development of the entire digestive tract of D. labrax, including the pharynx, was investigated from early embryonic development to day 5 post hatching (dph), when the mouth opens. The digestive tract is initialized at stage 12 somites independently from two distinct infoldings of the endodermal sheet. In the pharyngeal region, the anterior infolding forms the pharynx and the first gill slits at stage 25 somites. The other three gill arches and slits are formed between 1 and 5 dph. Posteriorly, in the gut tube region, a posterior infolding forms the foregut, midgut and hindgut. The anus opens before hatching, at stage 28 somites. Associated organs (liver, pancreas and gall bladder) are all discernable from 3 dph. Some aspects of the development of the two independent initial infoldings seem original compared with data in the literature. These results are discussed and compared with embryonic and post-embryonic development patterns in other teleosts.
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ISSN 0022-1112 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 543
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Auteur (down) Le Bihanic, F.; Clerandeau, C.; Cormier, B.; Crebassa, J.-C.; Keiter, S.H.; Beiras, R.; Morin, B.; Begout, M.-L.; Cousin, X.; Cachot, J.
Titre Organic contaminants sorbed to microplastics affect marine medaka fish early life stages development Type Article scientifique
Année 2020 Publication Revue Abrégée Mar. Pollut. Bull.
Volume 154 Numéro Pages 111059
Mots-Clés Benzo(a)pyrene; Benzophenone-3; Developmental toxicity; embryonic exposure; endocrine disruption; ingestion; Marine medaka; Microplastics; Perfluorooctanesulfonic acid; plastic pellets; pollutants; polycyclic aromatic-hydrocarbons; seine estuary; toxicity; water distribution; zebrafish
Résumé The role of polyethylene microplastics 4-6 mu m size (MPs) in the toxicity of environmental compounds to fish early life stages (ELS) was investigated. Marine medaka Oryzias melastigma embryos and larvae were exposed to suspended MPs spiked with three model contaminants: benzo(a)pyrene (MP-BaP), perfluorooctanesulfonic acid (MP-PFOS) and benzophenone-3 (MP-BP3) for 12 days. There was no evidence of MPs ingestion but MPs agglomerated on the surface of the chorion. Fish ELS exposed to virgin MPs did not show toxic effects. Exposure to MP-PFOS decreased embryonic survival and prevented hatching. Larvae exposed to MP-BaP or MP-BP3 exhibited reduced growth, increased developmental anomalies and abnormal behavior. Compared to equivalent water-borne concentrations, BaP and PFOS appeared to be more embryotoxic when spiked on MPs than when alone in seawater. These results suggest a relevant pollutant transfer by direct contact of MPs to fish ELS that should be included in the ecotoxicological risk assessment of MPs.
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Langue English Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 0025-326x ISBN Médium
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Notes WOS:000528205900032 Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2797
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Auteur (down) Cormier, B.; Batel, A.; Cachot, J.; Begout, M.-L.; Braunbeck, T.; Cousin, X.; Keiter, S.H.
Titre Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio) Type Article scientifique
Année 2019 Publication Revue Abrégée Front. Environ. Sci.
Volume 7 Numéro Pages 135
Mots-Clés aromatic-hydrocarbons pahs; benzo[a]pyrene; chronic dietary exposure; cyp1a; erod; fish embryotoxicity test (FET); fresh-water; gene-expression; hydrophobic organic-chemicals; marine-environment; oxybenzone; perfluorooctane sulfonate; plastic debris; resin pellets; risk-assessment; swimming behavior; uv-filters
Résumé As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a] pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP, and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63).
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Notes WOS:000486181200001 Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2646
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Auteur (down) Beiras, R.; Bellas, J.; Cachot, J.; Cormier, B.; Cousin, X.; Engwall, M.; Gambardella, C.; Garaventa, F.; Keiter, S.; Le Bihanic, F.; Lopez-Ibanez, S.; Piazza, V.; Rial, D.; Tato, T.; Vidal-Linan, L.
Titre Ingestion and contact with polyethylene microplastics does not cause acute toxicity on marine zooplankton Type Article scientifique
Année 2018 Publication Revue Abrégée J. Hazard. Mater.
Volume 360 Numéro Pages 452-460
Mots-Clés Benzophenone-3; coastal waters; ecotoxicological evaluation; Embryo-larval bioassays; environmental risk-assessment; isochrysis-galbana; larval development; Marine litter; Marine zooplankton; mytilus-galloprovincialis; organic pollutants; paracentrotus-lividus; plastic debris; Polyethylene; uv-filters
Résumé Toxicity of polyethylene microplastics (PE-MP) of size ranges similar to their natural food to zooplanktonic organisms representative of the main taxa present in marine plankton, including rotifers, copepods, bivalves, echinoderms and fish, was evaluated. Early life stages (ELS) were prioritized as testing models in order to maximize sensitivity. Treatments included particles spiked with benzophenone-3 (BP-3), a hydrophobic organic chemical used in cosmetics with direct input in coastal areas. Despite documented ingestion of both virgin and BP-3 spiked microplastics no acute toxicity was found at loads orders of magnitude above environmentally relevant concentrations on any of the invertebrate models. In fish tests some effects, including premature or reduced hatching, were observed after 12 d exposure at 10 mg L-1 of BP-3 spiked PE-MP. The results obtained do not support environmentally relevant risk of microplastics on marine zooplankton. Similar approaches testing more hydrophobic chemicals with higher acute toxicity are needed before these conclusions could be extended to other organic pollutants common in marine ecosystems. Therefore, the replacement of these polymers in consumer products must be carefully considered.
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Volume de collection Numéro de collection Edition
ISSN 0304-3894 ISBN Médium
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Numéro d'Appel MARBEC @ alain.herve @ collection 2432
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Auteur (down) Alfonso, S.; Blanc, M.; Joassard, L.; Keiter, S.H.; Munschy, C.; Loizeau, V.; Begout, M.-L.; Cousin, X.
Titre Examining multi- and transgenerational behavioral and molecular alterations resulting from parental exposure to an environmental PCB and PBDE mixture Type Article scientifique
Année 2019 Publication Revue Abrégée Aquat. Toxicol.
Volume 208 Numéro Pages 29-38
Mots-Clés adult zebrafish; Behavior; brominated flame retardants; developmental exposure; dietary-exposure; embryonic exposure; Epigenetic; Gene transcription; global dna methylation; left-right asymmetry; Offspring; Parental exposure; polybrominated diphenyl ethers; polychlorinated-biphenyls pcbs; Zebrafish; zebrafish danio-rerio
Résumé Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants extensively used during the 20th century and still present in aquatic environments despite their ban. Effects of exposure to these compounds over generations are poorly documented. Therefore, our aims were to characterize behavioral responses and underlying molecular mechanisms in zebrafish exposed to an environmentally relevant mixture of PCBs and PBDEs as well as in four unexposed offspring generations. Zebrafish (F0) were chronically exposed from the first meal onward to a diet spiked with a mixture containing 22 PCB and 7 PBDE congeners in proportions and concentrations reflecting environmental situations (Sigma PCBs = 1991 and Sigma PBDEs = 411 ng/g). Four offspring generations (F1 to F4) were obtained from this F0 and were not further exposed. Behavior was assessed at both larval and adult stages. Mechanisms related to behavioral defects (habenula maturation and c-fos transcription) and methylation (dnmts transcription) were monitored in larvae. Exposed adult F0 as well as F1 and F3 adults displayed no behavioral change while F2 expressed anxiety-like behavior. Larval behavior was also disrupted, Le. hyperactive after light to dark transition in F1 or hypoactive in F2, F3 and F4. Behavioral disruptions may be related to defect in habenula maturation (observed in F1) and change in c-fos transcription (observed in F1 and F2). Transcription of the gene encoding DNA methyltransferase (dnmt3ba) was also modified in all generations. Our results lead us to hypothesize that chronic dietary exposure to an environmentally relevant mixture of PCB and PBDE triggers multigenerational and transgenerational molecular and behavioral disruptions in a vertebrate model.
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Langue English Langue du Résumé Titre Original
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ISSN 0166-445x ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2549
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