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Auteur Fauvel, C.; Boryshpolets, S.; Cosson, J.; Leedy, J.G.W.; Labbe, C.; Haffray, P.; Suquet, M.
Titre Improvement of chilled seabass sperm conservation using a cell culture medium Type Article scientifique
Année 2012 Publication (up) Revue Abrégée J. Appl. Ichthyol.
Volume 28 Numéro 6 Pages 961-966
Mots-Clés dicentrarchus-labrax spermatozoa; fertilizing-capacity; fish; l. spermatozoa; motility; osmolality; psetta-maxima; quality; rainbow-trout; turbot scophthalmus-maximus
Résumé The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4 degrees C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.
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ISSN 0175-8659 ISBN Médium
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Auteur Wang, T.; Lefevre, S.; Iversen, N.K.; Findorf, I.; Buchanan, R.; McKenzie, D.J.
Titre Anaemia only causes a small reduction in the upper critical temperature of sea bass: is oxygen delivery the limiting factor for tolerance of acute warming in fishes? Type Article scientifique
Année 2014 Publication (up) Revue Abrégée Journal of Experimental Biology
Volume 217 Numéro 24 Pages 4275-4278
Mots-Clés aerobic scope; cardiac-performance; Cardiovascular; climate-change; dicentrarchus-labrax; ecology; exp. biol. 216; fish; Haematocrit; metabolism; Oxygen transport; phenylhydrazine-induced anemia; thermal tolerance; trout
Résumé To address how the capacity for oxygen transport influences tolerance of acute warming in fishes, we investigated whether a reduction in haematocrit, by means of intra-peritoneal injection of the haemolytic agent phenylhydrazine, lowered the upper critical temperature of sea bass. A reduction in haematocrit from 42 +/- 2% to 20 +/- 3% (mean +/- s.e.m.) caused a significant but minor reduction in upper critical temperature, from 35.8 +/- 0.1 to 35.1 +/- 0.2 degrees C, with no correlation between individual values for haematocrit and upper thermal limit. Anaemia did not influence the rise in oxygen uptake between 25 and 33 degrees C, because the anaemic fish were able to compensate for reduced blood oxygen carrying capacity with a significant increase in cardiac output. Therefore, in sea bass the upper critical temperature, at which they lost equilibrium, was not determined by an inability of the cardio-respiratory system to meet the thermal acceleration of metabolic demands.
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ISSN 0022-0949 ISBN Médium
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Notes <p>ISI Document Delivery No.: AW7BT<br/>Times Cited: 1<br/>Cited Reference Count: 44<br/>Wang, Tobias Lefevre, Sjannie Iversen, Nina K. Findorf, Inge Buchanan, Rasmus McKenzie, David J.<br/>Centre National de la Recherche Scientifique (CNRS); Danish Research Council; Region Languedoc-Roussillon (RLR); Ambassade de France in Copenhagen; Universite Montpellier 2<br/>This research was supported by the Centre National de la Recherche Scientifique (CNRS), the Danish Research Council, The Ambassade de France in Copenhagen and Universite Montpellier 2. T.W. was supported by a fellowship from Region Languedoc-Roussillon (RLR) as a visiting professor at Universite Montpellier 2. I.F. and N.K.I. were supported by a student grant from The Ambassade de France in Copenhagen.<br/>Company of biologists ltd<br/>Cambridge</p> Approuvé pas de
Numéro d'Appel MARBEC @ alain.herve @ collection 1181
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Auteur Blasco, F.R.; McKenzie, D.J.; Taylor, E.W.; Rantin, F.T.
Titre The role of the autonomic nervous system in control of cardiac and air-breathing responses to sustained aerobic exercise in the African sharptooth catfish Clarias gariepinus Type Article scientifique
Année 2017 Publication (up) Revue Abrégée Comp. Biochem. Physiol. A-Mol. Integr. Physiol.
Volume 203 Numéro Pages 273-280
Mots-Clés Adrenergic tone; bass dicentrarchus-labrax; cardiorespiratory interactions; Cholinergic tone; Fishes; Heart rate; heart-rate; hoplerythrinus-unitaeniatus; Hypoxia; oxygen-tensions; rainbow-trout; salmo-gairdneri; Swimming; synbranchus-marmoratus
Résumé Clarias gariepinus is a facultative air-breathing catfish that exhibits changes in heart rate (f(H)) associated with air breaths (AB). A transient bradycardia prior to the AB is followed by sustained tachycardia during breath-hold. This study evaluated air-breathing and cardiac responses to sustained aerobic exercise in juveniles (total length similar to 20 cm), and how exercise influenced variations in f(H) associated with AB. In particular, it investigated the role of adrenergic and cholinergic control in cardiac responses, and effects of pharmacological abolition of this control on air-breathing responses. Sustained exercise at 15, 30 and 45 cm s(-1) in a swim tunnel caused significant increases in f(AB) and f(H), from approximately 5 breaths h(-1) and 60 heartbeats min(-1) at the lowest speed, to over 60 breaths h(-1) and 100 beats min(-1) at the highest, respectively. There was a progressive decline in the degree of variation in f(H), around each AB, as f(AB) increased with exercise intensity. Total autonomic blockade abolished all variation in fH during exercise, and around each AB, but f(AB) responses were the same as in untreated animals. Cardiac responses were exclusively due to modulation of inhibitory cholinergic tone, which varied from >100% at the lowest speed to <10% at the highest. Cholinergic blockade had no effect on f(AB) compared to untreated fish. Excitatory beta-adrenergic tone was approximately 20% and did not vary with swimming speed, but its blockade increased f(AB) at all speeds, compared to untreated animals. This reveals complex effects of autonomic control on air-breathing during exercise in C. gariepinus, which deserve further investigation. (C) 2016 Elsevier Inc. All rights reserved.
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Volume de collection Numéro de collection Edition
ISSN 1095-6433 ISBN Médium
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Auteur Marras, S.; Killen, S.S.; Claireaux, G.; Domenici, P.; McKenzie, D.J.
Titre Behavioural and kinematic components of the fast-start escape response in fish: individual variation and temporal repeatability Type Article scientifique
Année 2011 Publication (up) Revue Abrégée J. Exp. Biol.
Volume 214 Numéro 18 Pages 3102-3110
Mots-Clés anaerobic performance; anaerobic swimming performance; body form; dicentrarchus-labrax; escape response; european sea bass; fast start; fish; gambusia-affinis; individual variation; locomotor performance; morphology; poecilia-reticulata; rainbow-trout; repeatability; sea bass; stereotype; swimming performance; teleost fish
Résumé Inter-individual variation in physiological performance traits, which is stable over time, can be of potential ecological and evolutionary significance. The fish escape response is interesting in this regard because it is a performance trait for which inter-individual variation may determine individual survival. The temporal stability of such variation is, however, largely unexplored. We quantified individual variation of various components of the escape response in a population of European sea bass (Dicentrarchus labrax), considering both non-locomotor (responsiveness and latency) and locomotor (speed, acceleration, turning rate, turning angle and distance travelled in a fixed time, D(esc)) variables. We assessed whether variation in performance was temporally stable and we searched for any trade-offs among the components of the response that might explain why the variation persisted in the population. The coefficient of variation was high for all components, from 23% for turning rate to 41% for D(esc), highlighting the non-stereotypic nature of the response. Individual performance for all variables was significantly repeatable over five sequential responses at 30min intervals, and also repeatable after a 30 day interval for most of the components. This indicates that the variation is intrinsic to the individuals, but there was no evidence for trade-offs amongst the components of the response, suggesting that, if trade-offs exist, they must be against other ecologically important behavioural or performance traits.
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Langue English Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 0022-0949 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 458
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Auteur L'Honore, T.; Farcy, E.; Chatain, B.; Gros, R.; Ruelle, F.; Hermet, S.; Blondeau-Bidet, E.; Naudet, J.; Lorin-Nebel, C.
Titre Are European sea bass as euryhaline as expected? Intraspecific variation in freshwater tolerance Type Article scientifique
Année 2019 Publication (up) Revue Abrégée Mar. Biol.
Volume 166 Numéro 8 Pages 102
Mots-Clés atlantic salmon; dicentrarchus-labrax; dna methylation; gene-expression; growth-hormone; messenger-rna levels; molecular characterization; phenotypic plasticity; stress-response; teleost fish
Résumé In teleosts, the regulation of hydromineral balance has a direct impact on several physiological functions, biochemical processes, and can influence behaviour, distribution and survival. As European sea bass Dicentrarchus labrax undertake seasonal migrations from seawater (SW) to brackish, estuarine and fresh water (FW) in their habitat, this study investigates their capacity to tolerate fresh water and explores intraspecific variations in physiological responses. Juvenile D. labrax were transferred from SW to FW at various ages. Freshwater-tolerant and non-tolerant phenotypes were discriminated according to behavioural and morphological characteristics. About 30% of the fish exposed to FW were identified as freshwater intolerant following FW challenges performed at different ages. Interestingly, intolerant fish exhibited the same phenotypic traits: erratic swimming, lower speed, isolation from the shoal and darker colour. Freshwater-intolerant fish were also characterised by a significant lower blood osmolality compared to tolerant fish, and significantly lower Na+/K+-ATPase alpha 1a expression in the posterior kidney. An imbalance in ion regulatory mechanisms was further confirmed by a blood Na+/Cl- ratio imbalance observed in some freshwater-intolerant fish. The analysis of glucocorticoid and mineralocorticoid receptor expression levels in gills and kidney revealed significant differences between freshwater-intolerant and -tolerant fish in both organs, suggesting differential stress-related responses. This study clearly shows an intraspecific difference in the responses following FW transfer with a decreased renal ion uptake capacity as a major cause for freshwater intolerance.
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Volume de collection Numéro de collection Edition
ISSN 0025-3162 ISBN Médium
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Notes WOS:000475657500006 Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2631
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