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Auteur Ramos-Judez, S.; Gonzalez, W.; Dutto, G.; Mylona, C.C.; Fauvel, C.; Duncan, N.
Titre Gamete quality and management for in vitro fertilisation in meagre (Argyrosomus regius) Type Article scientifique
Année 2019 Publication Revue Abrégée Aquaculture
Volume 509 Numéro Pages 227-235
Mots-Clés Argyrosomus regius; Artificial fertilisation; artificial fertilization; cultured fish; Gamete management; GnRHa; hatching rates; injections; Meagre; ovulation; Reproduction; sea-bass; sperm activation; spermatozoaoocyte ratio; time; water volume
Résumé The aquaculture of meagre (Argyrosomus regius) requires methods for the control of reproduction that enable the production of families from specific individuals for selective breeding programs. We experimentally determined the parameters required for an in vitro fertilisation protocol. A total of 14 females and 5 males (mean +/- S.D. weights of 20.45 +/- 6.22 and 15.94 +/- 2.75 kg, respectively) were used. Selected females had vitellogenic oocytes > 550 pm in diameter and males had fluid sperm upon application of abdominal pressure. Both sexes were treated with an injection of 15 mu g kg(-1) of gonadotropin-releasing hormone agonist (GnRHa) to induce oocyte maturation/ovulation and enhance sperm production. To determine the timing of ovulation and window of high egg viability, females were stripped serially every 2.5 h beginning 35 h after GnRHa treatment. Sperm was obtained 24 h after GnRHa treatment and was diluted 1/4 in modified Leibovitz for storage at 4 degrees C until use. Sperm quality parameters such as percentage initial spermatozoa motility, duration of motility, velocity and density were determined using computer assisted sperm analysis (CASA). In vitro inseminations were made in duplicate or triplicate batches of eggs from each spawn by mixing 0.5-1 mL of eggs, 20-40 mu L diluted sperm (pooled from two males) and 100 mL of seawater. Fertilisation success was examined at spermatozoa (spz): egg ratios between similar to 2000 and 400,000 spz egg(-1). The optimal time for stripping ovulated females was <= 3 h after ovulation, which was the window of optimal egg viability. Ovulation under the conditions of this study was close to 38 h after GnRHa treatment, with a range from 35 to 41 h. Beginning from 3 h after ovulation, egg viability declined probably due to overripening. Sperm diluted in Leibovitz maintained motility and velocity for as long as 7 h after collection. Spermatozoa motility (%) and average path velocity (VAP, mu m/s) of sperm samples obtained from males before GnRHa injection declined rapidly after activation compared to the samples obtained 24 h post-injection, with significant decreases respectively after 75 and 45 s. A minimum ratio of 150,000 spermatozoa egg(-1) was necessary to ensure high fertilisation success. The acquired knowledge of the present study will aid the aquaculture industry and future research on selective breeding programs for meagre.
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ISSN 0044-8486 ISBN Médium
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Notes WOS:000471749800029 Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2610
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Auteur Zupa, R.; Fauvel, C.; Mylonas, C.C.; Pousis, C.; Santamaria, N.; Papadaki, M.; Fakriadis, I.; Cicirelli, V.; Mangano, S.; Passantino, L.; Lacalandra, G.M.; Corriero, A.
Titre Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810) Type Article scientifique
Année 2017 Publication Revue Abrégée J. Anim. Sci.
Volume 95 Numéro 9 Pages 4085-4100
Mots-Clés acute stress; apoptosis; atlantic bluefin tuna; cultured fish; germ cell apoptosis; germ cell proliferation; germ-cell proliferation; greater amberjack; Marine fish; Motility; ovarian-steroid production; rearing in captivity; reproductive-biology; Seriola dumerili; Sperm quality; thunnus-thynnus l.
Résumé The greater amberjack, Seriola dumerili (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July). Spermatogonial stem cells and proliferating germ cells were identified through the immunohistochemical localization of Pou5f1 and proliferating cell nuclear antigen, respectively. Apoptotic germ cells were identified throughout the terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end labeling method. Sperm quality of captive-reared fish was evaluated using computer-assisted sperm analysis. Captive-reared males exhibited seminiferous lobules of a smaller diameter, a precocious and progressive decrease of spermatogonial mitosis, and a high level of apoptosis at the beginning of the reproductive season, concomitant with a many-fold higher 17 beta-estradiol plasma concentration. The motile spermatozoa percentage of captive greater amberjack was lower than in other teleosts, and a drastic decrease of spermatozoa motility duration, velocity, and ATP content occurred along the reproductive season. An abnormal increase of sperm concentration as well as an increase of dead spermatozoa occurred during the SPAWNING phase, probably because of lack of sperm hydration and ejaculation and consequent sperm ageing. The present study demonstrates the extreme susceptibility of greater amberjack to rearing stress and underscores the need for improvement of the rearing and handling procedures to ameliorate gametogenesis dysfunctions in commercial aquaculture production.
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Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0021-8812 ISBN Médium
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Numéro d'Appel MARBEC @ alain.herve @ collection 2210
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Auteur Zupa, R.; Fauvel, C.; Mylonas, C.C.; Santamaria, N.; Valentini, L.; Pousis, C.; Papadaki, M.; Suquet, M.; de la Gandara, F.; Bello, G.; Metrio, G.; Corriero, A.
Titre Comparative analysis of male germ cell proliferation and apoptosis in wild and captive Atlantic bluefin tuna (Thunnus thynnus L.) Type Article scientifique
Année 2013 Publication Revue Abrégée J. Appl. Ichthyol.
Volume 29 Numéro 1 Pages 71-81
Mots-Clés cultured fish; fish reproduction; halibut hippoglossus-hippoglossus; hormone agonist gnrha; rainbow-trout; reproductive maturation; salmo-salar; salmon; sertoli-cells; sperm motility; teleost fishes
Résumé The most commonly observed reproductive dysfunction in male fishes reared in captivity is reduction in sperm volume and quality. The Atlantic bluefin tuna Thunnus thynnus (Osteichthyes: Scombridae) is one of the few large pelagic and migratory marine fishes maintained in captivity with the purpose of establishing breeding populations to support an aquaculture industry. The objectives of the present study were to compare male germ cell proliferation and apoptosis between wild and captive individuals at two different phases of the spermatogenetic cycle, and to evaluate sperm motility characteristics of captive individuals. Histological observations were performed to analyze testicular activity, and germ cell proliferation and apoptosis were evaluated through the immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and the terminal deoxynucleotidyl transferasemediated d'UTP nick end labeling (TUNEL) method, respectively. Computer-assisted sperm analysis (CASA) was used to evaluate sperm motility. Results showed that germ cell proliferation was delayed and germ cell apoptosis increased in captive animals relative to wild individuals. Sperm motility of samples obtained from captive individuals was anomalous, both in terms of motility duration and swimming efficiency. Thus it appears that rearing in captivity impairs male reproductive function through, at least, changes in germ cell proliferation and apoptosis.
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ISSN 0175-8659 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 485
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