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Auteur Masroor, W.; Farcy, E.; Blondeau-Bidet, E.; Venn, A.; Tambutte, E.; Lorin-Nebel, C. doi  openurl
  Titre Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass Type Article scientifique
  Année 2019 Publication Revue Abrégée J. Therm. Biol.  
  Volume 85 Numéro Pages Unsp-102422  
  Mots-Clés cftr anion channel; chloride channel; cod gadus-morhua; Dicentrarchus labrax; dicentrarchus-labrax; fresh-water; functional classification; Gill; h+-atpase; Ion transporter; mRNA expression; na+/k+-atpase; na+/k+/2cl(-) cotransporter; Salinity; seawater-acclimated medaka; Thermal acclimation  
  Résumé The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 degrees C (temperate) or 24 degrees C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase ala (nka ala) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl--cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters involved in ion excretion (Na+/K+/2Cl-1( )cotransporter, nkcc1 and cystic fibrosis transmembrane conductance regulator, cftr) as well as nitrogen excretion (Rh-glycoproteins, rhcg1 and rhbg) and acid-base regulation (V-H+-ATPase, vha-a and b) are highly expressed in SW warm conditions vs FW warm. Overall, our results suggest a higher activation of ion transport processes in warm conditions and more strikingly in SW. This is linked to a strong interplay between diverse ion transporters in order to coordinate physiological responses at the gill level.  
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  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0306-4565 ISBN Médium  
  Région Expédition Conférence  
  Notes WOS:000497246100023 Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2669  
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Auteur Shirangi, S.A.; Kalbassi, M.R.; Khodabandeh, S.; Jafarian, H.; Lignot, J.-H. doi  openurl
  Titre Immunolocalization of Na+/K+-ATPase, Na+/K+/2Cl- co-transporter (NKCC) and mRNA expression of Na+/K+-ATPase +α-subunit during short-term salinity transfer in the gills of Persian sturgeon (Acipenser persicus, Borodin, 1897) juveniles Type Article scientifique
  Année 2017 Publication Revue Abrégée J. Appl. Ichthyol.  
  Volume 33 Numéro 3 Pages 347-353  
  Mots-Clés acclimation; bass dicentrarchus-labrax; cftr anion channel; fish; gene-expression; na+,k+,2cl(-) cotransporter; na+,k+-atpase; Osmoregulation; rich cells; salmon salmo-salar  
  Résumé The study tests the physiological responses of Persian sturgeon, Acipenser persicus, during the abrupt release of juveniles from freshwater (FW) into brackish waters (BW=11 parts per thousand) of the Caspian Sea. Fish weight at release was 2-3g (2.55 +/- 0.41g; 8.8 +/- 0.58cm TL). Totals of 160 individuals were randomly distributed into four fiber-glass aerated tanks (volume 60-L). Two tanks served as controls (FW groups), and two as exposure tanks for BW (Caspian Sea water=CSW). Fish were sampled at 0, 3, 6, 12, 24, 48 and 96hr after abrupt transfer to CSW. Plasma osmolality, immunolocalization of Na+, K+ -ATPase (NKA) and Na+/K+/2Cl(-) (NKCC) Co-transporter, NKA activity and the NKA -subunit mRNA expression were analyzed. Blood osmolality of fish transferred from FW to CSW increased significantly within hours post-transfer (p<.05) and remained at a high level for up to 96hr. Immunolocalization of NKCC indicated co-localization with NKA in the chloride cells in the gill epithelium. A partial sequence of the NKA -subunit (632bp) is described. Its expression levels were up-regulated at 12 and 48hr following salinity transfer (p<.05). However, NKA activity sharply increased in CSW specimens by almost 2.8-fold (p<.05) between 48 and 96hr after transfer. Gill NKCC co-transporter abundance increased, coinciding with increased gill NKA activity. The increased activity of NKCC during salt excretion in CSW may lead to an influx of Na+ into the chloride cells. Consequently, NKA activity increases to maintain intracellular Na+ homeostasis.  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0175-8659 ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 2152  
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