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Auteur Sucre, E.; Charmantier-Daures, M.; Grousset, E.; Charmantier, G.; Cucchi-Mouillot, P.
Titre Early development of the digestive tract (pharynx and gut) in the embryos and pre-larvae of the European sea bass Dicentrarchus labrax Type Article scientifique
Année 2009 Publication (down) Revue Abrégée J. Fish Biol.
Volume 75 Numéro 6 Pages 1302-1322
Mots-Clés Moronidae; arch; atlantic salmon; chloride cells; development; embryo; gill arches; gill slits; histology; medaka oryzias-latipes; ontogeny; oreochromis-niloticus; paralichthys-olivaceus embryos; rainbow-trout; respiratory development; urinary system; zebrafish
Résumé The European sea bass Dicentrarchus labrax is a marine teleost important in Mediterranean aquaculture. The development of the entire digestive tract of D. labrax, including the pharynx, was investigated from early embryonic development to day 5 post hatching (dph), when the mouth opens. The digestive tract is initialized at stage 12 somites independently from two distinct infoldings of the endodermal sheet. In the pharyngeal region, the anterior infolding forms the pharynx and the first gill slits at stage 25 somites. The other three gill arches and slits are formed between 1 and 5 dph. Posteriorly, in the gut tube region, a posterior infolding forms the foregut, midgut and hindgut. The anus opens before hatching, at stage 28 somites. Associated organs (liver, pancreas and gall bladder) are all discernable from 3 dph. Some aspects of the development of the two independent initial infoldings seem original compared with data in the literature. These results are discussed and compared with embryonic and post-embryonic development patterns in other teleosts.
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Langue English Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 0022-1112 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 543
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Auteur Iversen, N.K.; McKenzie, D.J.; Malte, H.; Wang, T.
Titre Reflex bradycardia does not influence oxygen consumption during hypoxia in the European eel (Anguilla anguilla) Type Article scientifique
Année 2010 Publication (down) Revue Abrégée J. Comp. Physiol. B-Biochem. Syst. Environ. Physiol.
Volume 180 Numéro 4 Pages 495-502
Mots-Clés Anguilla anguilla; Cardiac output; Critical oxygen tension; Hypoxic bradycardia; Oxygen; Teleost; atlantic cod; cardiac vagotomy; circulation; consumption; dogfish scyliorhinus-canicula; fish; heart-rate; rainbow-trout; responses; temperature; tolerance
Résumé Most teleost fish reduce heart rate when exposed to acute hypoxia. This hypoxic bradycardia has been characterised for many fish species, but it remains uncertain whether this reflex contributes to the maintenance of oxygen uptake in hypoxia. Here we describe the effects of inhibiting the bradycardia on oxygen consumption (MO(2)), standard metabolic rate (SMR) and the critical oxygen partial pressure for regulation of SMR in hypoxia (Pcrit) in European eels Anguilla anguilla (mean +/- A SEM mass 528 +/- A 36 g; n = 14). Eels were instrumented with a Transonic flow probe around the ventral aorta to measure cardiac output (Q) and heart rate (f (H)). MO(2) was then measured by intermittent closed respirometry during sequential exposure to various levels of increasing hypoxia, to determine Pcrit. Each fish was studied before and after abolition of reflex bradycardia by intraperitoneal injection of the muscarinic antagonist atropine (5 mg kg(-1)). In the untreated eels, f (H) fell from 39.0 +/- A 4.3 min(-1) in normoxia to 14.8 +/- A 5.2 min(-1) at the deepest level of hypoxia (2 kPa), and this was associated with a decline in Q, from 7.5 +/- A 0.8 mL min(-1) kg(-1) to 3.3 +/- A 0.7 mL min(-1) kg(-1) in normoxia versus deepest hypoxia, respectively. Atropine had no effect on SMR, which was 16.0 +/- A 1.8 mu mol O(2) kg(-1) min(-1) in control versus 16.8 +/- A 0.8 mu mol O(2) kg(-1) min(-1) following treatment with atropine. Atropine also had no significant effect on normoxic f (H) or Q in the eel, but completely abolished the bradycardia and associated decline in Q during progressive hypoxia. This pharmacological inhibition of the cardiac responses to hypoxia was, however, without affect on Pcrit, which was 11.7 +/- A 1.3 versus 12.5 +/- A 1.5 kPa in control versus atropinised eels, respectively. These results indicate, therefore, that reflex bradycardia does not contribute to maintenance of MO(2) and regulation of SMR by the European eel in hypoxia.
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Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0174-1578 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 805
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Auteur Cowart, D.A.; Durand, L.; Cambon-Bonavita, M.-A.; Arnaud-Haond, S.
Titre Investigation of bacterial communities within the digestive organs of the hydrothermal vent shrimp Rimicaris exoculata provide insights into holobiont geographic clustering Type Article scientifique
Année 2017 Publication (down) Revue Abrégée PLoS One
Volume 12 Numéro 3 Pages e0172543
Mots-Clés alignment; chamber; deposits; diversity; Ecology; microbial community; mid-atlantic ridge; population; sequence data; sp nov.
Résumé Prokaryotic communities forming symbiotic relationships with the vent shrimp, Rimicaris exoculata, are well studied components of hydrothermal ecosystems at the Mid-Atlantic Ridge (MAR). Despite the tight link between host and symbiont, the observed lack of spatial genetic structure seen in R. exoculata contrasts with the geographic differentiation detected in specific bacterial ectosymbionts. The geographic clustering of bacterial lineages within a seemingly panmictic host suggests either the presence of finer scale restriction to gene flow not yet detected in the host, horizontal transmission (environmental selection) of its endosymbionts as a consequence of unique vent geochemistry, or vertically transmitted endosymbionts that exhibit genetic differentiation. To identify which hypothesis best fits, we tested whether bacterial assemblages exhibit differentiation across sites or host populations by performing a 16S rRNA metabarcoding survey on R. exoculata digestive prokaryote samples (n = 31) taken from three geochemically distinct vents across MAR: Rainbow, Trans-Atlantic Geotraverse (TAG) and Logatchev. Analysis of communities across two organs (digestive tract, stomach), three molt colors (white, red, black) and three life stages (eggs, juveniles, adults) also provided insights into symbiont transmission mode. Examining both whole communities and operational taxonomic units (OTUs) confirmed the presence of three main epibionts: Epsilonproteobacteria, Mollicutes and Deferribacteres. With these findings, we identified a clear pattern of geographic segregation by vent in OTUs assigned to Epsilonproteobacteria. Additionally, we detected evidence for differentiation among all communities associated to vents and life stages. Overall, results suggest a combination of environmental selection and vertical inheritance of some of the symbiotic lineages.
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Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 1932-6203 ISBN Médium
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Numéro d'Appel MARBEC @ alain.herve @ collection 2085
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Auteur Caro, A.; Escalas, A.; Bouvier, C.; Grousset, E.; Lautredou-Audouy, N.; Roques, C.; Charmantier, M.; Gros, O.
Titre Epibiotic bacterial community of Sphaeroma serratum (Crustacea, Isopoda): relationship with molt status Type Article scientifique
Année 2012 Publication (down) Revue Abrégée Mar. Ecol.-Prog. Ser.
Volume 457 Numéro Pages 11-27
Mots-Clés 16s ribosomal-rna; Crustacean; DGGE band pattern; Epibiotic biofilm; Molt cycle; Sphaeroma; fish; gastropod; gradient gel-electrophoresis; hydrothermal-vent; in-situ hybridization; mid-atlantic ridge; oxidizing bacteria; phylogenetic diversity; riftia-pachyptila; shrimp rimicaris-exoculata; urothoe-poseidonis
Résumé Sphaeroma serratum is a marine isopod species that inhabits seashores from Europe to West Africa. The individuals live under stones in direct contact with reduced sediments and harbour a diverse bacterial community on the cuticle of their pleopods. We investigated the diversity of these epibiotic bacteria on male (pubescent and senescent) and female specimens with electron microscopic observations and molecular tools. The microbial community of S. serratum was shown to be composed of at least 5 bacterial morphotypes observed on the pleopodal cuticle in all male specimens. Using fluorescence in situ hybridization, we identified 5 major phylogenetic groups (alpha-, beta-, gamma- and delta-Proteobacteria and Archaea) whereas denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments of epibiotic bacteria revealed 50 bands. The bacterial community associated with S. serratum seems more diverse than in other marine crustaceans, such as Rimicaris. The relative diversity of this bacterial community was also studied in relation to the molt cycle. The comparison of DGGE band patterns of several individuals from female, pubescent male and senescent male groups revealed that the bacterial community diversity was dependent on the sex and the age of the individuals and more generally on the molt status.
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Editeur Lieu de Publication Éditeur
Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0171-8630 ISBN Médium
Région Expédition Conférence
Notes Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 564
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Auteur Amandé, M.; Ariz, J.; Chassot, E.; Molina, A.D. de; Gaertner, D.; Murua, H.; Pianet, R.; Ruiz, J.; Chavance, P.
Titre Bycatch of the European purse seine tuna fishery in the Atlantic Ocean for the 2003-2007 period Type Article scientifique
Année 2010 Publication (down) Revue Abrégée Aquatic Living Resources
Volume 23 Numéro 4 Pages 353-362
Mots-Clés atlantic Ocean; Bycatch; discards; Purse seining; Tuna fisheries
Résumé Bycatch of several groups of species and their characteristics are presented for the period 2003 to 2007 for the European purse seine tuna fishery operating in the Atlantic Ocean. Data were collected through French and Spanish observer programmes and represented a total of 27 trips corresponding to 2.9% coverage. Bycatch is defined as non-targeted species and small or damaged target species. Bycatch species composition, main species length, sex ratio and the fate of the most common species are presented first. Stratified ratios relative to landings of major commercial tunas were then used to estimate the total bycatch; these ratios were considered the most appropriate variable for extrapolation. Stratification was based on the fishing mode (free school vs. floating object), season (quarters) and spatial areas. The annual average bycatch was estimated at about 6400 t, corresponding to a mean annual value of 80.8 t per 1000 t of tuna landed or 7.5% of the total catch. Tunas represent 83% (67.2 t/1000 t) of the total bycatch, followed by other bony fishes (10%, 7.8 t/1000 t), billfishes (5%, 4.0 t/1000 t), sharks (1%, 0.9 t/1000 t) and rays (1%, 0.9 t/1000 t). Based on estimates of the annual bycatch, 16% was kept on board and sold in local markets.
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Langue Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 0990-7440 ISBN Médium
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Numéro d'Appel LL @ pixluser @ collection 56
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