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Auteur Espinosa, F.; Rivera-Ingraham, G.A.
Titre Subcellular evidences of redox imbalance in well-established populations of an endangered limpet. Reasons for alarm? Type Article scientifique
Année 2016 Publication Revue Abrégée Mar. Pollut. Bull.
Volume 109 Numéro 1 Pages 72-80
Mots-Clés antioxidant enzymes; community structure; Conservation; different environmental-conditions; Heavy metals; heavy-metals; marine-invertebrates; mussel mytilus-edulis; Oxidative stress; oyster crassostrea-virginica; Patella ferruginea; patella-ferruginea gastropoda; Pollution; trace-metals
Résumé Intertidal species are more vulnerable to anthropogenic disturbances than others inhabiting subtidal and offshore habitats. Coastal development frequently results in trace-metal pollution. For endangered species such as Patella ferruginea it can be a high risk that leads local populations to extinction. Three localities were surveyed, one within a natural and unpolluted area and the other two within the harbor of Ceuta (Strait of Gibraltar), on breakwaters outside and inside. The specimens collected inside the harbor reached 3-fold higher Hg content than for those incoming from the natural area. PERMANOVA test indicated that metal composition of the specimens from inside the harbor was different from the rest. In addition, evidence of cell damage was detected in the specimens from the harbor area. This highlights the urgency of undertaking a physiological evaluation of some of the most vulnerable populations, establishing eco-physiological protocols for monitoring and managing populations settled on artificial substrata. (C) 2016 Elsevier Ltd. All rights reserved.
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Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0025-326x ISBN Médium
Région Expédition Conférence
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Numéro d'Appel MARBEC @ alain.herve @ collection 1635
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Auteur Rivera-Ingraham, G.A.; Barri, K.; Boel, M.; Farcy, E.; Charles, A.-L.; Geny, B.; Lignot, J.-H.
Titre Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function? Type Article scientifique
Année 2016 Publication Revue Abrégée J. Exp. Biol.
Volume 219 Numéro 1 Pages 80-89
Mots-Clés Antioxidant defense; antioxidant enzymes; blue-crab; callinectes-sapidus; Carcinus aestuarii; crab carcinus-maenas; green crab; na+/k+-atpase activity; Osmoregulation; progressive hypoxia; reactive oxygen; ROS production; shore crab; temporal distribution
Résumé Osmoregulating decapods such as the Mediterranean green crab Carcinus aestuarii possess two groups of spatially segregated gills: anterior gills serve mainly respiratory purposes, while posterior gills contain osmoregulatory structures. The co-existence of similar tissues serving different functions allows the study of differential adaptation, in terms of free radical metabolism, upon salinity change. Crabs were immersed for 2 weeks in seawater (SW, 37 ppt), diluted SW (dSW, 10 ppt) and concentrated SW (cSW, 45 ppt). Exposure to dSW was the most challenging condition, elevating respiration rates of whole animals and free radical formation in hemolymph (assessed fluorometrically using C-H(2)DFFDA). Further analyses considered anterior and posterior gills separately, and the results showed that posterior gills are the main tissues fueling osmoregulatory-related processes because their respiration rates in dSW were 3.2-fold higher than those of anterior gills, and this was accompanied by an increase in mitochondrial density (citrate synthase activity) and increased levels of reactive oxygen species (ROS) formation (1.4-fold greater, measured through electron paramagnetic resonance). Paradoxically, these posterior gills showed undisturbed caspase 3/7 activity, used here as a marker for apoptosis. This may only be due to the high antioxidant protection that posterior gills benefit from [superoxide dismutase (SOD) in posterior gills was over 6 times higher than in anterior gills]. In conclusion, osmoregulating posterior gills are better adapted to dSW exposure than respiratory anterior gills because they are capable of controlling the deleterious effects of the ROS production resulting from this salinity-induced stress.
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Auteur institutionnel Thèse
Editeur Lieu de Publication Éditeur
Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0022-0949 ISBN Médium
Région Expédition Conférence
Notes Approuvé pas de
Numéro d'Appel MARBEC @ alain.herve @ collection 1541
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