bascule de visibilité Search & Display Options

Tout Sélectionner    Désélectionner
 |   | 
Détails
   print
  Enregistrements Liens
Auteur (down) Masroor, W.; Farcy, E.; Gros, R.; Lorin-Nebel, C. doi  openurl
  Titre Effect of combined stress (salinity and temperature) in European sea bass Dicentrarchus labrax osmoregulatory processes Type Article scientifique
  Année 2018 Publication Revue Abrégée Comp. Biochem. Physiol. A-Mol. Integr. Physiol.  
  Volume 215 Numéro Pages 45-54  
  Mots-Clés Osmoregulation; fresh-water; Teleost; goldfish carassius-auratus; Ionocytes; branchial na+/k+-atpase; fish gills; gill morphology; Gill plasticity; Hydromineral balance; k+-atpase activity; mitochondria-rich cells; mozambique tilapia; NKA activity; salmon salmo-salar; Thermal acclimation; tilapia oreochromis-mossambicus  
  Résumé European sea bass Dicentrarchus Iabrax undertake seasonal migrations to estuaries and lagoons that are characterized by fluctuations in environmental conditions. Their ability to cope with these unstable habitats is undeniable, but it is still not clear how and to what extent salinity acclimation mechanisms are affected at temperatures higher than in the sea. In this study, juvenile sea bass were pre-acclimated to seawater (SW) at 18 degrees C (temperate) or 24 degrees C (warm) for 2 weeks and then transferred to fresh water (FW) or SW at the respective temperature. Transfer to FW for two weeks resulted in decreased blood osmolalities and plasma Cl- at both temperatures. In FW warm conditions, plasma Na+ was similar to 15% lower and Cl- was similar to 32% higher than in the temperate-water group. Branchial Na+/K+-ATPase (NKA) activity measured at the acclimation temperature (V-apparent) did not change according to the conditions. Branchial Na+/K+-ATPase activity measured at 37 degrees C (V-max) was lower in warm conditions and increased in FW compared to SW conditions whatever the considered temperature. Mitochondrion-rich cell (MRC) density increased in FW, notably due to the appearance of lamellar MRCs, but this increase was less pronounced in warm conditions where MRC's size was lower. In SW warm conditions, pavement cell apical microridges are less developed than in other conditions. Overall gill morphometrical parameters (filament thickness, lamellar length and width) differ between fish that have been pre acclimated to different temperatures. This study shows that a thermal change affects gill plasticity affecting whole-organism ion balance two weeks after salinity transfer.  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 1095-6433 ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 2260  
Lien permanent pour cet enregistrement
 

 
Auteur (down) Masroor, W.; Farcy, E.; Blondeau-Bidet, E.; Venn, A.; Tambutte, E.; Lorin-Nebel, C. doi  openurl
  Titre Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass Type Article scientifique
  Année 2019 Publication Revue Abrégée J. Therm. Biol.  
  Volume 85 Numéro Pages Unsp-102422  
  Mots-Clés cftr anion channel; chloride channel; cod gadus-morhua; Dicentrarchus labrax; dicentrarchus-labrax; fresh-water; functional classification; Gill; h+-atpase; Ion transporter; mRNA expression; na+/k+-atpase; na+/k+/2cl(-) cotransporter; Salinity; seawater-acclimated medaka; Thermal acclimation  
  Résumé The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 degrees C (temperate) or 24 degrees C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase ala (nka ala) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl--cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters involved in ion excretion (Na+/K+/2Cl-1( )cotransporter, nkcc1 and cystic fibrosis transmembrane conductance regulator, cftr) as well as nitrogen excretion (Rh-glycoproteins, rhcg1 and rhbg) and acid-base regulation (V-H+-ATPase, vha-a and b) are highly expressed in SW warm conditions vs FW warm. Overall, our results suggest a higher activation of ion transport processes in warm conditions and more strikingly in SW. This is linked to a strong interplay between diverse ion transporters in order to coordinate physiological responses at the gill level.  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0306-4565 ISBN Médium  
  Région Expédition Conférence  
  Notes WOS:000497246100023 Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2669  
Lien permanent pour cet enregistrement
Tout Sélectionner    Désélectionner
 |   | 
Détails
   print

Save Citations:
Export Records: