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Auteur (up) Kengne, P.; Charmantter, G.; Blondeau-Bidet, E.; Costantini, C.; Ayala, D. doi  openurl
  Titre Tolerance of disease-vector mosquitoes to brackish water and their osmoregulatory ability Type Article scientifique
  Année 2019 Publication Revue Abrégée Ecosphere  
  Volume 10 Numéro 10 Pages e02783  
  Mots-Clés adaptation; Aedes; aedes-aegypti; Anopheles; anopheles-gambiae; Culex; growth; hemolymph; hemolymph osmolality; invertebrates; larval; lethal concentration; localization; mosquitoes; osmoregulation; rectal secretion; salinity tolerance; salt  
  Résumé Salinity tolerance is an important trait that governs the ecology of disease-vector mosquitoes by determining their choice of larval habitat, and consequently their ecological and geographical distribution. Here, we used laboratory strains to determine the osmotic responses of larvae of obligate freshwater disease-vector mosquitoes (Aedes aegypti, Aedes albopictus, Anopheles coluzzii, An. gambiae, Culex pipiens, and Cx. quinquefasciatus) and assessed their relationship with salinity tolerance. First, we analyzed the acute dose-mortality response of fourth-instar larvae to salinity; then, we measured their hemolymph osmolality after 24-h exposure to varying salinities. We found that Ae. albopictus was the most tolerant species, followed by An. coluzzii, Ae. aegypti, Cx. quinquefasciatus, and An. gambiae, in decreasing order. Cx. pipiens was the least tolerant species. All mosquitoes were hyper-iso-osmoregulators, but with species-specific differences. Specifically, hemolymph osmolality in deionized water varied among species, and Cx. pipiens and the two Aedes species showed the lowest and highest osmolality. Although all species were osmoconformers at higher salinity values, hemolymph osmolality approached environmental osmolality more rapidly in species of the Culex genus, compared with Aedes species where it increased slowly. Moreover, hemolymph osmolality in deionized water was significantly correlated with tolerance to salinity across species. This could allow predicting the salinity tolerance of untested species on the basis of their osmoregulatory ability. However, this correlation disappeared when considering the hemolymph osmolality of larvae exposed to salinities higher than deionized water.  
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  ISSN 2150-8925 ISBN Médium  
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  Notes WOS:000493528200019 Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2671  
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Auteur (up) Lorin-Nebel, C.; Avarre, J.C.; Faivre, N.; Wallon, S.; Charmantier, G.; Durand, J.D. url  doi
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  Titre Osmoregulatory strategies in natural populations of the black-chinned tilapia Sarotherodon melanotheron exposed to extreme salinities in West African estuaries Type Article scientifique
  Année 2012 Publication Revue Abrégée J. Comp. Physiol. B-Biochem. Syst. Environ. Physiol.  
  Volume 182 Numéro 6 Pages 771-780  
  Mots-Clés ATPase; Gills; Hypersalinity; Na+/ K+; Osmoregulation; Teleost; Tilapia; cichlidae; expression; fish; gambia; gill; life-history traits; pisces; saloum estuaries; water  
  Résumé The effect of salinity was studied in natural populations of the black-chinned tilapia (Sarotherodon melanotheron) from West Africa. This euryhaline species colonizes nearly all coastal environments from bays to lagoons characterized by salinities ranging from fresh water to hypersaline water over 100 aEuro degrees. Individuals were sampled during the dry season at several locations characterized by different levels of salinity (3-102 aEuro degrees). Their osmotic status and their gills were analyzed. The branchial mitochondria-rich cells (MRC), localized at the basis of the filaments and along the lamellae in fish taken from the saline stations, showed a wide plasticity with significant differences in their number and size. The most striking results were a significant larger area (a parts per thousand 3x) and a higher number (a parts per thousand 55x) of MRC at high salinity (102 aEuro degrees) compared to low salinity (3 aEuro degrees). The major ion transporters and channels were localized by immunocytochemistry and different expression patterns have been recorded between stations. Despite an increased Na+/K+-ATPase (NKA) alpha-subunit expression and NKA activity, pointing to an increased monovalent ion excretion, a severe osmotic imbalance was recorded in animals living in hypersaline environments.  
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  ISSN 0174-1578 ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 748  
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Auteur (up) Masroor, W.; Farcy, E.; Gros, R.; Lorin-Nebel, C. doi  openurl
  Titre Effect of combined stress (salinity and temperature) in European sea bass Dicentrarchus labrax osmoregulatory processes Type Article scientifique
  Année 2018 Publication Revue Abrégée Comp. Biochem. Physiol. A-Mol. Integr. Physiol.  
  Volume 215 Numéro Pages 45-54  
  Mots-Clés Osmoregulation; fresh-water; Teleost; goldfish carassius-auratus; Ionocytes; branchial na+/k+-atpase; fish gills; gill morphology; Gill plasticity; Hydromineral balance; k+-atpase activity; mitochondria-rich cells; mozambique tilapia; NKA activity; salmon salmo-salar; Thermal acclimation; tilapia oreochromis-mossambicus  
  Résumé European sea bass Dicentrarchus Iabrax undertake seasonal migrations to estuaries and lagoons that are characterized by fluctuations in environmental conditions. Their ability to cope with these unstable habitats is undeniable, but it is still not clear how and to what extent salinity acclimation mechanisms are affected at temperatures higher than in the sea. In this study, juvenile sea bass were pre-acclimated to seawater (SW) at 18 degrees C (temperate) or 24 degrees C (warm) for 2 weeks and then transferred to fresh water (FW) or SW at the respective temperature. Transfer to FW for two weeks resulted in decreased blood osmolalities and plasma Cl- at both temperatures. In FW warm conditions, plasma Na+ was similar to 15% lower and Cl- was similar to 32% higher than in the temperate-water group. Branchial Na+/K+-ATPase (NKA) activity measured at the acclimation temperature (V-apparent) did not change according to the conditions. Branchial Na+/K+-ATPase activity measured at 37 degrees C (V-max) was lower in warm conditions and increased in FW compared to SW conditions whatever the considered temperature. Mitochondrion-rich cell (MRC) density increased in FW, notably due to the appearance of lamellar MRCs, but this increase was less pronounced in warm conditions where MRC's size was lower. In SW warm conditions, pavement cell apical microridges are less developed than in other conditions. Overall gill morphometrical parameters (filament thickness, lamellar length and width) differ between fish that have been pre acclimated to different temperatures. This study shows that a thermal change affects gill plasticity affecting whole-organism ion balance two weeks after salinity transfer.  
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  ISSN 1095-6433 ISBN Médium  
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  Numéro d'Appel MARBEC @ alain.herve @ collection 2260  
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Auteur (up) Maugars, G.; Manirafasha, M.-C.; Grousset, E.; Boulo, V.; Lignot, J.-H. doi  openurl
  Titre The effects of acute transfer to freshwater on ion transporters of the pharyngeal cavity in European seabass (Dicentrarchus labrax) Type Article scientifique
  Année 2018 Publication Revue Abrégée Fish Physiol. Biochem.  
  Volume 44 Numéro 5 Pages 1393-1408  
  Mots-Clés atlantic salmon; atpase alpha-1 isoforms; branchial chloride cells; carbonic-anhydrase; Dicentrarchus labrax; gill na+/k+-atpase; Gills and extrabranchial organs; Ion transporters; k+-atpase; mitochondrion-rich cells; Osmoregulation; salinity transfer; salmon salmo-salar; seawater acclimation; Seawater to freshwater transfer; Teleost fish  
  Résumé Gene expression of key ion transporters (the Na+/K+-ATPase NKA, the Na+, K+-2Cl(-) cotransporter NKCC1, and CFTR) in the gills, opercular inner epithelium, and pseudobranch of European seabass juveniles (Dicentrarchus labrax) were studied after acute transfer up to 4days from seawater (SW) to freshwater (FW). The functional remodeling of these organs was also studied. Handling stress (SW to SW transfer) rapidly induced a transcript level decrease for the three ion transporters in the gills and operculum. NKA and CFTR relative expression level were stable, but in the pseudobranch, NKCC1 transcript levels increased (up to 2.4-fold). Transfer to FW induced even more organ-specific responses. In the gills, a 1.8-fold increase for NKA transcript levels occurs within 4days post transfer with also a general decrease for CFTR and NKCC1. In the operculum, transcript levels are only slightly modified. In the pseudobranch, there is a transient NKCC1 increase followed by 0.6-fold decrease and 0.8-fold CFTR decrease. FW transfer also induced a density decrease for the opercular ionocytes and goblet cells. Therefore, gills and operculum display similar trends in SW-fish but have different responses in FW-transferred fish. Also, the pseudobranch presents contrasting response both in SW and in FW, most probably due to the high density of a cell type that is morphologically and functionally different compared to the typical gill-type ionocyte. This pseudobranch-type ionocyte could be involved in blood acid-base regulation masking a minor osmotic regulatory capacity of this organ compared to the gills.  
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  Volume de collection Numéro de collection Edition  
  ISSN 0920-1742 ISBN Médium  
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  Numéro d'Appel MARBEC @ alain.herve @ collection 2417  
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Auteur (up) Pham, D.; Charmantier, G.; Boulo, V.; Wabete, N.; Ansquer, D.; Dauga, C.; Grousset, E.; Labreuche, Y.; Charmantier-Daures, M. url  doi
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  Titre Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae): Deciphering the role of the Na+/K+-ATPase Type Article scientifique
  Année 2016 Publication Revue Abrégée Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology  
  Volume 196–197 Numéro Pages 27-37  
  Mots-Clés Litopenaeus stylirostris; Na+/K+-ATPase; Ontogeny; Osmoregulation; Penaeid; RNA interference  
  Résumé The role of the main ion transporting enzyme Na +/K +-ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the mRNA coding for the α-subunit of Na + K +-ATPase was characterized in L. stylirostris. The resulting 3092-bp cDNA (LsNKA) encodes a putative 1011-amino-acid protein with a predicted molecular mass of 112.3 kDa. The inferred amino acid sequence revealed that the putative protein possesses the main structural characteristics of the Na + K +-ATPase α-subunits. Quantitative RT-PCR analyses indicated that LsNKA transcripts did not significantly vary between the different developmental stages. The number of transcripts was about 2.5-fold higher in the epipodites and gills than in any other tissues tested in juveniles. A reverse genetic approach was finally implemented to study the role of LsNKA in vivo. Knockdown of LsNKA expression by gene-specific dsRNA injection led to an increase of shrimp mortality following an abrupt salinity change compared to control animals. These data strongly suggest that LsNKA plays an important role in osmoregulation when the shrimp are challenged by changing salinities.  
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  Volume de collection Numéro de collection Edition  
  ISSN 1096-4959 ISBN Médium  
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  Numéro d'Appel MARBEC @ alain.herve @ collection 1481  
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