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Auteur (up) Kiilerich, P.; Geffroy, B.; Valotaire, C.; Prunet, P.
Titre Endogenous regulation of 11-deoxycorticosterone (DOC) and corticosteroid receptors (CRs) during rainbow trout early development and the effects of corticosteroids on hatching Type Article scientifique
Année 2018 Publication Revue Abrégée General and Comparative Endocrinology
Volume Numéro Pages
Mots-Clés 11-Deoxycorticosterone; Cortisol; Development; Fish; Glucocorticoid receptor; Mineralocorticoid receptor
Résumé Clear evidence for a physiological role of the mineralocorticoid-like hormone 11-deoxycorticosterone (DOC) and the mineralocorticoid receptor (MR) in fish is still lacking. Efforts to demonstrate an osmoregulatory role for this hormone has so far not been conclusive, while a few scattered studies have indicated a role for DOC in development and reproduction. In this study, we investigate the onset of de novo DOC synthesis in parallel with endogenous corticosteroid receptor mRNA production from fertilization to the swim-up stage in rainbow trout. Whole egg DOC content decreased from fertilization until hatching followed by an increase to pre-fertilization levels just after hatching. Onset of de novo transcription of corticosteroid receptor mRNA’s was observed shortly after the midblastula transition; initially glucocorticoid receptor 2 (GR2) followed by MR and then GR1. Non-invasive introduction of DOC or cortisol at fertilization resulted in altered corticosteroid receptor regulation and accelerated hatching date, suggesting a regulatory role in trout ontogenesis of both hormones through MR signaling pathway. The results presented in this study suggest a possible physiological role of the DOC-MR signaling pathway during fish ontogenesis, at fertilization and just after hatching.
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ISSN 0016-6480 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2363
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Auteur (up) Sadoul, B.; Alfonso, S.; Bessa, E.; Bouchareb, A.; Blondeau-Bidet, E.; Clair, P.; Chatain, B.; Begout, M.L.; Geffroy, B.
Titre Enhanced brain expression of genes related to cell proliferation and neural differentiation is associated with cortisol receptor expression in fishes Type Article scientifique
Année 2018 Publication Revue Abrégée Gen. Comp. Endocrinol.
Volume 267 Numéro Pages 76-81
Mots-Clés behavior; dehydrogenase; european sea bass; Glucocorticoid receptor; glucocorticoid-receptor; Hypothalamo-pituitary-interrenal axis; Mineralocorticoid receptor; neurogenesis; Neurogenesis; plasticity; rainbow-trout; real-time pcr; stress; Stress; zebrafish
Résumé Stress enhances or inhibits neurogenesis in mammals and some fish species. The link between the two processes is still unclear. Most studies have been performed in very specific stressful or altered environments. Despite the known inter-individual divergence in coping abilities within populations, the relationship between the stress axis and neurogenesis has never been addressed in unstressed individuals. Here we correlate brain expression of the pcna (proliferating cell nuclear antigen) and neurod1 (neurogenic differentiation factor 1) genes, two markers of neurogenesis, with transcripts of cortisol receptors in three fish species living in very distinct environments. Within the three species, individuals with the highest expression of neurogenesis genes were also those that expressed the high levels of cortisol receptors. Based on these correlations and the hypothesis that mRNA levels are proxies of protein levels, we hypothesize that within unstressed animals, individuals sensitive to cortisol perceive a similar environment to be more stimulating, leading to increased neurogenesis. Although it is difficult to determine whether it is sensitivity to cortisol that affects neurogenesis capacities or the opposite, the proposed pathway is a potentially fruitful avenue that warrants further mechanistic experiments.
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Auteur institutionnel Thèse
Editeur Lieu de Publication Éditeur
Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0016-6480 ISBN Médium
Région Expédition Conférence
Notes Approuvé pas de
Numéro d'Appel MARBEC @ alain.herve @ collection 2416
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