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Auteur Boukef, I.; El Bour, M.; Al Gallas, N.; El Bahri, O.; Mejri, S.; Mraouna, R.; Ben Aissa, R.; Boudabous, A.; Got, P.; Troussellier, M.
Titre Survival of Escherichia coli Strains in Mediterranean Brackish Water in the Bizerte Lagoon in Northern Tunisia Type Article scientifique
Année 2010 Publication Revue Abrégée Water Environ Res
Volume 82 Numéro 11 Pages 2249-2257
Mots-Clés bizerte lagoon; brackish water; coastal waters; cytotoxicity; enteric bacteria; escherichia coli; fecal-coliforms; marine waters; salmonella-typhimurium; seawater microcosms; sewage; shigella-dysenteriae type-1; sunlight; survival; viability; virulence
Résumé This study investigated survival and virulence of Escherichia coli strains exposed to natural conditions in brackish water. Two E. coli strains (O126:B16 and O55:B5) were incubated in water microcosms in the Bizerte lagoon in Northern Tunisia and exposed for 12 days to natural sunlight in June (231 to 386 W/m(2), 26 +/- 1 degrees C, 30 g/L) and in April (227 to 330 W/m(2), 17 +/- 1 degrees C, 27 g/L) or maintained in darkness for 21 days (17 +/- 1 degrees C, 27 g/L). The results revealed that sunlight was the most significant inactivating factor (decrease of 3 Ulog within 48 hours for the two strains) compared to salinity and temperature (in darkness). Survival time of the strains was prolonged as they were maintained in darkness. Local strain (E. coli O55:B5) showed better survival capacity (T(90) = 52 hours) than E. coli O126:B16 (T(90) = 11 h). For both, modifications were noted only for some metabolic activities of carbohydrates hydrolysis. Cytotoxicity of the two strains, tested on Vero cell, was maintained during the period of survival. Water Environ. Res., 82, 2249 (2010).
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ISSN 1061-4303 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 864
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Auteur Louati, H.; Ben Said, O.; Soltani, A.; Got, P.; Mahmoudi, E.; Cravo-Laureau, C.; Duran, R.; Aissa, P.; Pringault, O.
Titre The roles of biological interactions and pollutant contamination in shaping microbial benthic community structure Type Article scientifique
Année 2013 Publication Revue Abrégée Chemosphere
Volume 93 Numéro 10 Pages 2535-2546
Mots-Clés Bioremediation; Meiofauna; Microcosms; PAH-degraders; bacteria; bizerte lagoon
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ISSN 0045-6535 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 894
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Auteur Louati, H.; Said, O.B.; Got, P.; Soltani, A.; Mahmoudi, E.; Cravo-Laureau, C.; Duran, R.; Aissa, P.; Pringault, O.
Titre Microbial community responses to bioremediation treatments for the mitigation of low-dose anthracene in marine coastal sediments of Bizerte lagoon (Tunisia) Type Article scientifique
Année 2013 Publication Revue Abrégée Environ Sci Pollut Res
Volume 20 Numéro 1 Pages 300-310
Mots-Clés Anthracene; Microbial communities; Microcosms; Oxygen microprofiles; T-RFLP analysis; bizerte lagoon
Résumé PURPOSE: The main goals of this study were to investigate (1) the behavior of microbial communities in response to low-dose bioavailable anthracene addition in lightly contaminated sediment from Bizerte Lagoon and (2) the effects of bioremediation treatments on microbial biomass, activity, and community structure. METHODS: Sediment microcosms amended with 1 ppm anthracene were incubated in triplicate during 30 days. Biostimulation (addition of nitrogen and phosphorus fertilizer) and bioaugmentation (inoculation of a hydrocarbonoclastic bacterium) were used as bioremediation treatments. Bacterial biomass was estimated using flow cytometry. Sediment oxygen consumption was measured with oxygen microelectrodes. Bacterial community structure was assessed by molecular fingerprints (terminal restriction fragment length polymorphism; T-RFLP) analysis. RESULTS: Anthracene contamination resulted in a significant reduction of bacterial abundance with an impact on cell integrity. Concomitantly, sediment oxygen consumption was strongly inhibited. Correspondence analysis on T-RFLP data indicated that bacterial community structures from anthracene-contaminated microcosms were different from that of the control. Interestingly, the changes observed in microbial biomass, structure, and activities as a result of anthracene contamination were not alleviated even with the use of biostimulation and combination of biostimulation and bioaugmentation strategy for anthracene bioremediation. Nevertheless, both treatment methods resulted in different community structures relative to the contaminated and control microcosms with the appearance of distinct populations. CONCLUSION: Anthracene spiking severely affected microbial communities, suggesting dominance of nontolerant populations in this lightly-contaminated sediment. Although biostimulation and/or bioaugmentation treatments did not alleviate the anthracene toxic effects, the changes observed in microbial population and structure suggest that the proposed treatments might be promising to promote bacterial growth. Further works are still required to propose a more efficient strategy to stimulate biodegradation that takes into account the complex interactions between species for resource access.
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Volume de collection Numéro de collection Edition
ISSN 0944-1344 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 716
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Auteur Pringault, O.; Lafabrie, C.; Avezac, M.; Bancon-Montigny, C.; Carré, C.; Chalghaf, M.; Delpoux, S.; Duvivier, A.; Elbaz-Poulichet, F.; Gonzalez, C.; Got, P.; Leboulanger, C.; Spinelli, S.; Hlaili, A.S.; Bouvy, M.
Titre Consequences of contaminant mixture on the dynamics and functional diversity of bacterioplankton in a southwestern Mediterranean coastal ecosystem Type Article scientifique
Année 2016 Publication Revue Abrégée Chemosphere
Volume 144 Numéro Pages 1060-1073
Mots-Clés aquatic systems; Bacterial growth efficiency; Bacterial production; bizerte lagoon; Community level physiological profiles; growth efficiency; Herbicides; heterotrophic bacteria; Metals; microbial communities; Microcosms; organic-carbon; polycyclic aromatic-hydrocarbons; sediment resuspension; substrate utilization pattern; surface sediments
Résumé Contamination of coastal environments is often due to a complex mixture of pollutants, sometimes in trace levels, that may have significant effects on diversity and function of organisms. The aim of this study was to evaluate the short-term dynamics of bacterioplankton exposed to natural and artificial mixtures of contaminants. Bacterial communities from a southwestern Mediterranean ecosystem, lagoon and the bay (offshore) of Bizerte were exposed to i) elutriate from resuspension of contaminated sediment, and ii) an artificial mixture of metals and herbicides mimicking the contamination observed during sediment resuspension. Elutriate incubation as well as artificial spiking induced strong enrichments in nutrients (up to 18 times), metals (up to six times) and herbicides (up to 20 times) relative to the in situ concentrations in the offshore station, whereas the increases in contaminants were less marked in the lagoon station. In the offshore waters, the artificial mixture of pollutants provoked a strong inhibition of bacterial abundance, production and respiration and significant modifications of the potential functional diversity of bacterioplankton with a strong decrease of the carbohydrate utilization. In contrast, incubation with elutriate resulted in a stimulation of bacterial activities and abundances, suggesting that the toxic effects of pollutants were modified by the increase in nutrient and DOM concentrations due to the sediment resuspension. The effects of elutriate and the artificial mixture of pollutants on bacterial dynamics and the functional diversity were less marked in the lagoon waters, than in offshore waters, suggesting a relative tolerance of lagoon bacteria against contaminants. (C) 2015 Elsevier Ltd. All rights reserved.
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Volume de collection Numéro de collection Edition
ISSN 0045-6535 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 1507
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Auteur Pringault, O.; Viret, H.; Duran, R.
Titre Influence of microorganisms on the removal of nickel in tropical marine sediments (New Caledonia) Type Article scientifique
Année 2010 Publication Revue Abrégée Marine Pollution Bulletin
Volume 61 Numéro 7–12 Pages 530-541
Mots-Clés Adsorption; Metal spiking; Microcosms; T-RFLP analysis
Résumé The removal of nickel in marine tropical sediments (New Caledonia) was studied in microcosms. Removal of Ni2+ was strongly enhanced by the presence of bacteria, with rates up to twofold higher than those observed under sterilized conditions. After 8 days of incubation, Ni2+ concentration in the water column ranged from 30% to 50% of the initial concentration according to sediment origin. Addition of glucose stimulated bacterial processes and resulted in a complete disappearance of Ni2+ in the water phase. Incubation under anoxic conditions slightly affects the microbial structure inferred from T-RFLP analysis irrespective of Ni2+ spiking, whereas incubation under oxic conditions resulted to moderate modification of the microbial structure, changes that might be more marked in the presence of Ni2+. Five different T-RFs were observed in almost all microcosms with relative abundance between 5% and 30%. Incubation with glucose resulted in the dominance of a common T-RF, with relative abundance up to 39%.
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Volume de collection Numéro de collection Edition
ISSN 0025-326x ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 685
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