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Auteur BROSSET, P.; FROMENTIN, J.-M.; MENARD, F.; PERNET, F.; BOURDEIX, J.-H.; BIGOT, J.-L.; VAN BEVEREN, E.; PEREZ RODIA, M.A.; CHOY, S.; SARAUX, C.
Titre Measurement and analysis of small pelagic fish condition: A suitable method for rapid evaluation in the field Type Article scientifique
Année 2015 Publication Journal Of Experimental Marine Biology And Ecology Revue Abrégée (up)
Volume 462 Numéro Pages 90-97
Mots-Clés Anchovy; Fatmeter; Lipids; Mediterranean Sea; Sardine; Sprat
Résumé As condition is a key variable in population dynamics (especially for survival, growth and reproduction), the use of well-defined and accurate fish condition indices is capital. In particular, condition indices (morphometric, bioenergetic and biochemical) have never been compared and validated for the European anchovy Engraulis encrasicolus, the European pilchard Sardina pilchardus and the European sprat Sprattus sprattus. The accuracy of two indirect methods, the morphometric relative condition index Kn and the bioenergetics index determined with the Distell Fish Fatmeter was investigated by comparing with a direct measure of relative lipid content carried out with a thin layer chromatography-flame ionization detector. Estimations from the fatmeter correlated quite well with the relative lipid contents of all species, regardless of the reproductive period (R2 = 0.69 for anchovy, R2 = 0.75 for sprat and R2 = 0.48 for sardine). Kn correlated more poorly with relative lipid content (R2 = 0.22 for anchovy and R2 = 0.41 for sardine, ns for sprat), especially during the reproductive period, pointing out the difficulty for such an index to precisely reflect changes in fat allocation. During the reproductive period, changes in Kn could reflect other processes, such as changes in protein content. Therefore, these different types of commonly used indices do not reflect exactly the same type of energy stores. The high repeatability of the fatmeter was brought to light, so that only one measurement on each fish side may be necessary to evaluate the relative lipid content of a small pelagic fish. Finally, fatmeter measurements were not affected by freezing storage up to one month for anchovy (R2 = 0.66) and sardine (R2 = 0.90), making it possible to use frozen samples of both commercial and scientific survey. In contrast, the freezing storage for sprat should be avoided. Based on this study, the Fatmeter appears to be a suitable indirect method to assess condition and fat content of sardine and anchovy on a large number of individuals.
Adresse IFREMER, Laboratoire des sciences de l'Environnement Marin, LEMAR, Technopole Brest Iroise, BP 70, 29280 Plouzané, France
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Editeur Lieu de Publication Éditeur
Langue Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0022-0981 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ 33741 collection 984
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Auteur Siddique, M.A.M.; Butts, I.A.E.; Linhart, O.; Macias, A.D.; Fauvel, C.
Titre Fertilization strategies for Sea Bass Dicentrarchus labrax (Linnaeus, 1758): effects of pre-incubation and duration of egg receptivity in seawater Type Article scientifique
Année 2015 Publication Revue Abrégée (up) Aquac Res
Volume Numéro Pages 1-9
Mots-Clés Dicentrarchus labrax; egg longevity; Mediterranean sea bass; overripe eggs; reproductive ecology
Résumé Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives of this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min and then fertilized by pooled sperm from four males. The fresh eggs had a higher fertilization success than overripe eggs. Our results revealed a significant effect of pre-incubation time for both the fresh (P < 0.01) and overripe eggs (P < 0.01). Fertilization success of eggs significantly declined for both these treatments after 3 min of pre-incubation, which clearly indicates that sea bass eggs are able to be fertilized by sperm for up to 3 min after release into seawater. This study has particular importance for understanding fertilization strategies, reproductive potential, as well as reproductive ecology of sea bass.
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Langue en Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 1365-2109 ISBN Médium
Région Expédition Conférence
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 1456
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Auteur Richard, M.; Bourreau, J.; Montagnani, C.; Ouisse, V.; Le Gall, P.; Fortune, M.; Munaron, D.; Messiaen, G.; Callier, M.D.; Roque d'Orbcastel, E.
Titre Influence of OSHV-1 oyster mortality episode on dissolved inorganic fluxes: An ex situ experiment at the individual scale Type Article scientifique
Année 2017 Publication Revue Abrégée (up) Aquaculture
Volume 475 Numéro Pages 40-51
Mots-Clés carrying-capacity; Crassostrea gigas; crassostrea-gigas spat; eastern oyster; juvenile; la-madeleine quebec; mediterranean thau lagoon; Mineralisation; mortality; mussel mytilus-edulis; mu-var; Nutrient fluxes; Ostreid herpesvirus 1; ostreid herpesvirus-1 infection; oxygen consumption; oxygen-consumption rates; pacific oysters; Spat
Résumé Ostreid herpesvirus 1 (OsHV-1 mu var) infection has caused significant mortalities in juvenile oysters (Crassostrea gigas). In contrast to the practices of other animal production industries, sick and dead oysters are not separated from live ones and are left to decay in the surrounding environment, with unknown consequences on fluxes of dissolved materials. A laboratory approach was used in this study to test the influence of oyster mortality episode on dissolved inorganic fluxes at the oyster interface, dissociating (i) the effect of viral infection on metabolism of juvenile oysters and (ii) the effect of flesh decomposition on oxygen consumption and nutrient releases at the individual scale. Nine batches of juvenile oysters (Individual Total wet weight 1 g) were infected via injection of OsHV-1 enriched inoculums at different viral loads (108 and 109 OsHV-1 DNA copies per oyster) to explore infection thresholds. Oysters injected with filtered seawater were used as controls (C). Oysters were maintained under standard conditions to avoid stress linked to hypoxia, starvation, or ammonia excess. Before, after the injection and during the mortality episode, i.e. at days 1, 3, 7, 10 and 14, nine oysters per treatment were incubated in individual metabolic chambers to quantify oxygen, ammonium and phosphate fluxes at the seawater-oyster interface. Nine empty chambers served as a reference. Injections of the two viral loads of OsHV-1 induced similar mortality rates (38%), beginning at day 3 and lasting until day 14. The observed mortality kinetics were slower than those reported in previous experimental pathology studies, but comparable to those observed in the field (Thau lagoon, France). This study highlights that oxygen and nutrient fluxes significantly varied during mortality episode. Indeed (i) OsHV-1 infection firstly modifies oyster metabolism, with significant decreases in oxygen consumption and ammonium excretion, and (ii) dead oysters lead to a strong increase of ammonium (6 fold) and phosphate (41 fold) fluxes and a decrease in the N/P ratio due to mineralisation of their flesh. The latter may modify the structure of the planktonic community in the field during mortality episode. This study is a first step of the MORTAFLUX program. The second step was to in situ confirm this abnormal nutrient loading during a mortality episode and show its impact on bacterio-, phyto-and protozoo-plankton. (C) 2017 Elsevier B.V. All rights reserved.
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Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0044-8486 ISBN Médium
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Numéro d'Appel MARBEC @ alain.herve @ collection 2149
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Auteur Panfili, J.; Darnaude, A.M.; Lin, Y.J.; Chevalley, M.; Iizuka, Y.; Tzeng, W.N.; Crivelli, A.J.
Titre Habitat residence during continental life of the European eel Anguilla anguilla investigated using linear discriminant analysis applied to otolith Sr:Ca ratios Type Article scientifique
Année 2012 Publication Revue Abrégée (up) Aquat. Biol.
Volume 15 Numéro 2 Pages 175-185
Mots-Clés Anguilla anguilla; Epma; European eel; La-Icpms; Mediterranean area; Otolith; Strontium:calcium ratio; coastal; elemental fingerprints; estuarine; fish otoliths; fresh-water environments; history; japonica; mass; microchemistry; rostrata; spectrometry; sr-ca ratios
Résumé European eel Anguilla anguilla migratory behaviour during continental life is still unclear due to the multiplicity of aquatic environments colonised by the species. In the Camargue area (NW Mediterranean), eel colonisation of the Fumemorte canal, a freshwater habitat that communicates only with a vast brackish ecosystem (the Vaccares lagoon), offers a rare opportunity to test for freshwater habitat residence during continental life. To this end, both laser ablation inductively coupled plasma mass spectrometry and electron probe micro-analysis were used to measure chronological variations of strontium concentrations (Sr:Ca) in the otoliths of 58 silver eels captured in the canal between 1997 and 2007. Comparing mean Sr: Ca ratios measured on otolith edges with the 2 analytical methods indicated that they provide comparable measurement accuracies. Linear discriminant analysis (LDA), based on the otolith Sr: Ca values corresponding to the initial entrance of the fish into the brackish ecosystem and their final capture in the freshwater canal, allowed successful discrimination of the 2 habitats and reconstruction of migratory history for all individuals. Six different migratory behaviours were identified. Eels that entered the freshwater canal did so either directly (67%) or after 1 to 2 yr spent in the lagoon (33%), with a subsequent majority of freshwater residents (55%) until their silvering. These results indicate the value of LDA for reconstructing habitat use during continental life using Sr: Ca ratios. They confirm the occurrence of freshwater residence during continental life in European eels, even in Mediterranean continental areas where brackish environments are predominant. This observed sedentary behaviour has implications for eel population management and conservation.
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Editeur Lieu de Publication Éditeur
Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 1864-7790 ISBN Médium
Région Expédition Conférence
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 626
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Auteur Blanchet, M.; Pringault, O.; Panagiotopoulos, C.; Lefevre, D.; Charriere, B.; Ghiglione, J.-F.; Fernandez, C.; Aparicio, F.L.; Marrase, C.; Catala, P.; Oriol, L.; Caparros, J.; Joux, F.
Titre When riverine dissolved organic matter (DOM) meets labile DOM in coastal waters: changes in bacterial community activity and composition Type Article scientifique
Année 2017 Publication Revue Abrégée (up) Aquat. Sci.
Volume 79 Numéro 1 Pages 27-43
Mots-Clés Bacterial community composition; bacterioplankton; biodegradation; carbon; coastal waters; Dissolved organic matter; Fluorescence; growth efficiency; lake water; marine; Mediterranean Sea; open-ocean; Priming effect; rhone river; terrestrial
Résumé Heterotrophic bacterial communities in marine environments are exposed to a heterogeneous mixture of dissolved organic compounds with different bioreactivity that may control both their activity and composition. The coastal environment is an example of a mixing area where recalcitrant allochthonous organic matter from rivers can encounter labile organic matter from marine phytoplanktonic blooms. The objective of this study was to explore the effects of mixed qualities of dissolved organic matter (DOM) on bacterial community activity (BCA) and bacterial community composition (BCC) and to test for a priming effect when DOM sources are added in combination. Coastal marine bacterial communities were incubated separately with a mixture of amino acids and with natural riverine DOM or with both sources together for 42 days. Addition of amino acids alone or in combination with riverine DOM led to a similar stimulation of BCA compared to control condition, whereas addition of riverine DOM alone did not modify BCA compared to the control. On the contrary, BCC analyzed by 16S rRNA gene pyrosequencing was not affected by the addition of amino acids alone, but changed dramatically with riverine DOM alone or in combination with amino acids. Our results show that changes in BCA and BCC can be driven by different types of DOM, but that these changes are not necessarily coupled. Moreover, the addition of labile DOM did not modify the microbial decomposition of riverine DOM, nor the BCC, suggesting that a priming effect did not occur under these experimental conditions.
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Editeur Lieu de Publication Éditeur
Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 1015-1621 ISBN Médium
Région Expédition Conférence
Notes Approuvé pas de
Numéro d'Appel MARBEC @ alain.herve @ collection 2090
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