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Auteur (up) Boudour-Boucheker, N.; Boulo, V.; Lorin-Nebel, C.; Elguero, C.; Grousset, E.; Anger, K.; Charmantier-Daures, M.; Charmantier, G. url  doi
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  Titre Adaptation to freshwater in the palaemonid shrimp Macrobrachium amazonicum: comparative ontogeny of osmoregulatory organs Type Article scientifique
  Année 2013 Publication Revue Abrégée Cell Tissue Res  
  Volume 353 Numéro 1 Pages 87-98  
  Mots-Clés Antennal gland; Branchial chamber; Crustaceans; Immunolocalization; Macrobrachium amazonicum; Na+/K+-ATPase; Osmoregulation  
  Résumé  
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  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0302-766x ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 441  
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Auteur (up) Dayras, P.; Charmantier, G.; Chaumot, A.; Vigneron, A.; Coquery, M.; Quéau, H.; Artells, E.; Lignot, J.-H.; Geffard, O.; Issartel, J. url  doi
openurl 
  Titre Osmoregulatory responses to cadmium in reference and historically metal contaminated Gammarus fossarum (Crustacea, Amphipoda) populations Type Article scientifique
  Année 2017 Publication Revue Abrégée Chemosphere  
  Volume 180 Numéro Pages 412-422  
  Mots-Clés Cadmium; Crustacean; Histology; immunolocalization; Na+/K+-ATPase; V-H+-ATPase  
  Résumé In order to better understand the variable sensitivities of crustaceans to metals, we investigated the impact of cadmium exposure in 3 populations of Gammarus fossarum from different rivers of France. The first population lives in a Cd-contaminated river from a geochemical background, while the others inhabit Cd-free sites. Osmoregulation, a relevant biomarker to evaluate crustacean health following metal contamination, was used as a proxy to evaluate the intra- and inter-populationnal sensitivities to Cd. Specimens from each population were experimentally exposed to 9 μg Cd2+/L Cd for 7 days and hemolymph osmolality (HO) was then individually measured. In exposed populations, high inter-individual variations in HO values were noted, resulting in their separation into non-impacted and slightly or highly Cd-impacted (with lower HO) animals. In gills of impacted organisms, deep histopathological alterations and protein overexpression of Na+/K+-ATPase and V-H+-ATPase were observed through histology and immunolocalization, while non-impacted animals showed profiles comparable to controls. Moreover, the osmoregulatory processes in the population living in the Cd-contaminated site were impacted by acute Cd exposure in the laboratory as much as for one of the two populations originating from Cd-free sites. The observed changes did not reveal any obvious adaptive osmoregulatory phenomena at the population scale, but they may be due to differences in fitness between individuals and between populations in relation to the features of their respective environments, unrelated with the presence of the metal.  
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  Langue Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0045-6535 ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 2104  
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Auteur (up) Gerber, L.; Lee, C.E.; Grousset, E.; Blondeau-Bidet, E.; Boucheker, N.B.; Lorin-Nebel, C.; Charmantier-Daures, M.; Charmantier, G. doi  openurl
  Titre The Legs Have It: In Situ Expression of Ion Transporters V-Type H+-ATPase and Na+/K+-ATPase in the Osmoregulatory Leg Organs of the Invading Copepod Eurytemora affinis Type Article scientifique
  Année 2016 Publication Revue Abrégée Physiol. Biochem. Zool.  
  Volume 89 Numéro 3 Pages 233-250  
  Mots-Clés crab chasmagnathus-granulatus; crustacea; euryhaline crabs; fresh-water crab; immunolocalization; integumental windows; ionic regulation; malpighian tubules; messenger-rna expression; Na+/K+-ATPase; Osmoregulation; posterior gills; salinity acclimation; shrimp macrobrachium-amazonicum; swimming legs; V-type H+-ATPase  
  Résumé The copepod Eurytemora affinis has an unusually broad salinity range, as some populations have recently invaded freshwater habitats independently from their ancestral saline habitats. Prior studies have shown evolutionary shifts in ion transporter activity during freshwater invasions and localization of ion transporters in newly discovered “Crusalis organs” in the swimming legs. The goals of this study were to localize and quantify expression of ion transport enzymes V-type H+-ATPase (VHA) and Na+/K+-ATPase (NKA) in the swimming legs of E. affinis and determine the degree of involvement of each leg in ionic regulation. We confirmed the presence of two distinct types of ionocytes in the Crusalis organs. Both cell types expressed VHA and NKA, and in the freshwater population the location of VHA and NKA in ionocytes was, respectively, apical and basal. Quantification of in situ expression of NKA and VHA established the predominance of swimming leg pairs 3 and 4 in ion transport in both saline and freshwater populations. Increases in VHA expression in swimming legs 3 and 4 of the freshwater population (in fresh water) relative to the saline population (at 15 PSU) arose from an increase in the abundance of VHA per cell rather than an increase in the number of ionocytes. This result suggests a simple mechanism for increasing ion uptake in fresh water. In contrast, the decline in NKA expression in the freshwater population arose from a decrease in ionocyte area in legs 4, likely resulting from decreases in number or size of ionocytes containing NKA. Such results provide insights into mechanisms of ionic regulation for this species, with added insights into evolutionary mechanisms underlying physiological adaptation during habitat invasions.  
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  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 1522-2152 ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 1622  
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Auteur (up) Sellami, I.; Charmantier, G.; Naceur, H.B.; Kacem, A.; Lorin-Nebel, C. doi  openurl
  Titre Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia) Type Article scientifique
  Année 2020 Publication Revue Abrégée Tissue Cell  
  Volume 63 Numéro Pages Unsp-101340  
  Mots-Clés alpha-subunit; Artemia salina; brine shrimp artemia; fresh-water; homarus-gammarus crustacea; Hypo-osmoregulation; Immunolocalization; ionic regulation; Maxillary glands; Metepipodites; na+,k+-atpase; Na+/K+ -ATPase; ontogeny; osmotic regulation; potential difference; salt-gland  
  Résumé Artemia salina is an extremophile species that tolerates a wide range of salinity, especially hypertonic media considered lethal for the majority of other aquatic species. In this study, A. salina cysts were hatched in the laboratory and nauplii were acclimated at three different salinities (60, 139 and 212 ppt). Once in the adult phase, their hemolymph osmolality was measured. The animals were strong hypo-osmoregulators in the entire range of tested salinities, with up to 10 fold lower hemolymph osmolalities than their surrounding environment. Immunostaining of Na+/K+-ATPase was done on sections and on whole body mounts of adults in order to localize the ionocytes in different organs. An intense Na+/K+-ATPase immunostaining throughout the cells was observed in the epithelium of the ten pairs of metepipodites. A positive immunoreactivity for Na+/K+-ATPase was also detected in the maxillary glands, in the epithelium of the efferent tubule and of the excretory canal, as well as in the anterior digestive tract. This study confirms the strong hypo-osmotic capacity of this species and affords an overview of the different organs involved in osmoregulation in A. salina adults.  
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  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0040-8166 ISBN Médium  
  Région Expédition Conférence  
  Notes WOS:000522853900012 Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2758  
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