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Auteur (up) Goikoetxea, A.; Sadoul, B.; Blondeau-Bidet, E.; Aerts, J.; Blanc, M.-O.; Parrinello, H.; Barrachina, C.; Pratlong, M.; Geffroy, B. url  doi
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  Titre Genetic pathways underpinning hormonal stress responses in fish exposed to short- and long-term warm ocean temperatures Type Article scientifique
  Année 2021 Publication Revue Abrégée Ecological Indicators  
  Volume 120 Numéro Pages 106937  
  Mots-Clés Commercial fish; Cortisol; Glucocorticoid receptors; Scales; Transcriptomics  
  Résumé Changes in ocean water temperature associated with global climate change are bound to enormously affect fish populations, with potential major economic consequences in the aquaculture and fisheries industries. A link between temperature fluctuations and changes in fish stress response is well established. In this study, we aimed to assess the effects of a short- (4 days) or a long-term (4 months) exposure to warm temperature in the stress physiology of European sea bass (Dicentrarchus labrax) larvae and juveniles. First, cortisol (i.e. the main stress hormone in fishes) analysis was used to confirm that a steady and short-term elevation of temperature acts as a physiological stressful event in these fish, and cortisol release is indeed above a metabolic increase linked to temperature. Moreover, our results verified that measurement of cortisol released into the water can be reliably employed as a non-invasive indicator of acute thermal stress in experimental conditions. Secondly, the different effects on the genetic cascade underlying the stress response between long-term low or high thermal treatments were evaluated at two larval development stages via candidate-gene and whole-transcriptome approaches. Interestingly, opposite expression for some key stress genes (nr3c1, nr3c2 and hsd11b2) were observed between developmental stages, highlighting the distinct adaptive mechanisms controlling the primary and secondary responses to a stressor. Surprising expression patterns for some understudied genes involved in the stress axis were also revealed, including crhr1, mc2r, mc5r, trh or trhr, which should be further explored. Finally, evaluation of cortisol content in scales was successfully used as a biomarker of chronic thermal stress, with 10x more cortisol in fish kept at 21 °C vs 16 °C after 4 months, supporting the gene expression results observed. The use of such a method as a proxy of long-term stress, unprecedented in the literature, holds a vast array of applications in further research, in particular, in the investigation of the impact of global warming on wild fish populations.  
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  Editeur Lieu de Publication Éditeur  
  Langue en Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 1470-160x ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2828  
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Auteur (up) Kiilerich, P.; Geffroy, B.; Valotaire, C.; Prunet, P. url  doi
openurl 
  Titre Endogenous regulation of 11-deoxycorticosterone (DOC) and corticosteroid receptors (CRs) during rainbow trout early development and the effects of corticosteroids on hatching Type Article scientifique
  Année 2018 Publication Revue Abrégée General and Comparative Endocrinology  
  Volume Numéro Pages  
  Mots-Clés 11-Deoxycorticosterone; Cortisol; Development; Fish; Glucocorticoid receptor; Mineralocorticoid receptor  
  Résumé Clear evidence for a physiological role of the mineralocorticoid-like hormone 11-deoxycorticosterone (DOC) and the mineralocorticoid receptor (MR) in fish is still lacking. Efforts to demonstrate an osmoregulatory role for this hormone has so far not been conclusive, while a few scattered studies have indicated a role for DOC in development and reproduction. In this study, we investigate the onset of de novo DOC synthesis in parallel with endogenous corticosteroid receptor mRNA production from fertilization to the swim-up stage in rainbow trout. Whole egg DOC content decreased from fertilization until hatching followed by an increase to pre-fertilization levels just after hatching. Onset of de novo transcription of corticosteroid receptor mRNA’s was observed shortly after the midblastula transition; initially glucocorticoid receptor 2 (GR2) followed by MR and then GR1. Non-invasive introduction of DOC or cortisol at fertilization resulted in altered corticosteroid receptor regulation and accelerated hatching date, suggesting a regulatory role in trout ontogenesis of both hormones through MR signaling pathway. The results presented in this study suggest a possible physiological role of the DOC-MR signaling pathway during fish ontogenesis, at fertilization and just after hatching.  
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  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0016-6480 ISBN Médium  
  Région Expédition Conférence  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2363  
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Auteur (up) Sadoul, B.; Alfonso, S.; Bessa, E.; Bouchareb, A.; Blondeau-Bidet, E.; Clair, P.; Chatain, B.; Begout, M.L.; Geffroy, B. doi  openurl
  Titre Enhanced brain expression of genes related to cell proliferation and neural differentiation is associated with cortisol receptor expression in fishes Type Article scientifique
  Année 2018 Publication Revue Abrégée Gen. Comp. Endocrinol.  
  Volume 267 Numéro Pages 76-81  
  Mots-Clés behavior; dehydrogenase; european sea bass; Glucocorticoid receptor; glucocorticoid-receptor; Hypothalamo-pituitary-interrenal axis; Mineralocorticoid receptor; neurogenesis; Neurogenesis; plasticity; rainbow-trout; real-time pcr; stress; Stress; zebrafish  
  Résumé Stress enhances or inhibits neurogenesis in mammals and some fish species. The link between the two processes is still unclear. Most studies have been performed in very specific stressful or altered environments. Despite the known inter-individual divergence in coping abilities within populations, the relationship between the stress axis and neurogenesis has never been addressed in unstressed individuals. Here we correlate brain expression of the pcna (proliferating cell nuclear antigen) and neurod1 (neurogenic differentiation factor 1) genes, two markers of neurogenesis, with transcripts of cortisol receptors in three fish species living in very distinct environments. Within the three species, individuals with the highest expression of neurogenesis genes were also those that expressed the high levels of cortisol receptors. Based on these correlations and the hypothesis that mRNA levels are proxies of protein levels, we hypothesize that within unstressed animals, individuals sensitive to cortisol perceive a similar environment to be more stimulating, leading to increased neurogenesis. Although it is difficult to determine whether it is sensitivity to cortisol that affects neurogenesis capacities or the opposite, the proposed pathway is a potentially fruitful avenue that warrants further mechanistic experiments.  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0016-6480 ISBN Médium  
  Région Expédition Conférence  
  Notes Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 2416  
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