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Auteur Rivera-Ingraham, G.A.; Rocchetta, I.; Bickmeyer, U.; Meyer, S.; Abele, D. url  doi
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  Titre Spatial compartmentalization of free radical formation and mitochondrial heterogeneity in bivalve gills revealed by live-imaging techniques Type Article scientifique
  Année 2016 Publication (up) Revue Abrégée Frontiers in Zoology  
  Volume 13 Numéro Pages 4  
  Mots-Clés Bivalve; Fluorescence; Gill; Live-imaging; Mitochondria; Rns; Ros  
  Résumé Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level.  
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  ISSN 1742-9994 ISBN Médium  
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  Notes Pages 1-17 in PDF Approuvé pas de  
  Numéro d'Appel MARBEC @ alain.herve @ collection 1542  
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Auteur Gros, O.; Elisabeth, N.H.; Gustave, S.D.D.; Caro, A.; Dubilier, N. url  doi
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  Titre Plasticity of symbiont acquisition throughout the life cycle of the shallow-water tropical lucinid Codakia orbiculata (Mollusca: Bivalvia) Type Article scientifique
  Année 2012 Publication (up) Revue Abrégée Environ. Microbiol.  
  Volume 14 Numéro 6 Pages 1584-1595  
  Mots-Clés bacteria; bathymodiolus-azoricus; cold; endosymbiont transmission; gill-endosymbionts; identification; in-situ hybridization; population; seeps; sulfur; vertical transmission  
  Résumé In marine invertebrates that acquire their symbionts from the environment, these are generally only taken up during early developmental stages. In the symbiosis between lucinid clams and their intracellular sulfur-oxidizing bacteria, it has been shown that the juveniles acquire their symbionts from an environmental stock of free-living symbiont forms, but it is not known if adult clams are still competent to take up symbiotic bacteria from the environment. In this study, we investigated symbiont acquisition in adult specimens of the lucinid clam Codakia orbiculata, using transmission electron microscopy, fluorescence in situ hybridization, immunohistochemistry and PCR. We show here that adults that had no detectable symbionts after starvation in aquaria for 6 months, rapidly reacquired symbionts within days after being returned to their natural environments in the field. Control specimens that were starved and then exposed to seawater aquaria with sulfide did not reacquire symbionts. This indicates that the reacquisition of symbionts in the starved clams returned to the field was not caused by high division rates of a small pool of remaining symbionts that we were not able to detect with the methods used here. Immunohistochemistry with an antibody against actin, a protein involved in the phagocytosis of intracellular bacteria, showed that actin was expressed at the apical ends of the gill cells that took up symbionts, providing further evidence that the symbionts were acquired from the environment. Interestingly, actin expression was also observed in symbiont-containing cells of untreated lucinids freshly collected from the environment, indicating that symbiont acquisition from the environment occurs continuously in these clams throughout their lifetime.  
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  ISSN 1462-2912 ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 784  
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Auteur Boudour-Boucheker, N.; Boulo, V.; Charmantier-Daures, M.; Grousset, E.; Anger, K.; Charmantier, G.; Lorin-Nebel, C. url  doi
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  Titre Differential distribution of V-type H+-ATPase and Na+/K+-ATPase in the branchial chamber of the palaemonid shrimp Macrobrachium amazonicum Type Article scientifique
  Année 2014 Publication (up) Revue Abrégée Cell and Tissue Research  
  Volume 357 Numéro 1 Pages 195-206  
  Mots-Clés Branchiostegite; Gills; Na+/K+-ATPase; Osmoregulation; V-type H+-ATPase; crab eriocheir-sinensis; decapoda; epithelial potential difference; fresh-water crab; gill epithelium; homarus-gammarus; ion-transport; larval development; lobster; olfersii; plasma-membrane; salinity acclimation  
  Résumé V-H+-ATPase and Na+/K+-ATPase were localized in the gills and branchiostegites of M. amazonicum and the effects of salinity on the branchial chamber ultrastructure and on the localization of transporters were investigated. Gills present septal and pillar cells. In freshwater (FW), the apical surface of pillar cells is amplified by extensive evaginations associated with mitochondria. V-H+-ATPase immunofluorescence was localized in the membranes of the apical evaginations and in clustered subapical areas of pillar cells, suggesting labeling of intracellular vesicle membranes. Na+/K+-ATPase labeling was restricted to the septal cells. No difference in immunostaining was recorded for both proteins according to salinity (FW vs. 25 PSU). In the branchiostegite, both V-H+-ATPase and Na+/K+-ATPase immunofluorescence were localized in the same cells of the internal epithelium. Immunogold revealed that V-H+-ATPase was localized in apical evaginations and in electron-dense areas throughout the inner epithelium, while Na+/K+-ATPase occurred densely along the basal infoldings of the cytoplasmic membrane. Our results suggest that morphologically different cell types within the gill lamellae may also be functionally specialized. We propose that, in FW, pillar cells expressing V-H+-ATPase absorb ions (Cl-, Na+) that are transported either directly to the hemolymph space or through a junctional complex to the septal cells, which may be responsible for active Na+ delivery to the hemolymph through Na+/K+-ATPase. This suggests a functional link between septal and pillar cells in osmoregulation. When shrimps are transferred to FW, gill and branchiostegite epithelia undergo ultrastructural changes, most probably resulting from their involvement in osmoregulatory processes.  
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  ISSN 0302-766x ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 541  
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Auteur Sucre, E.; Charmantier-Daures, M.; Grousset, E.; Charmantier, G.; Cucchi-Mouillot, P. url  doi
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  Titre Early development of the digestive tract (pharynx and gut) in the embryos and pre-larvae of the European sea bass Dicentrarchus labrax Type Article scientifique
  Année 2009 Publication (up) Revue Abrégée J. Fish Biol.  
  Volume 75 Numéro 6 Pages 1302-1322  
  Mots-Clés Moronidae; arch; atlantic salmon; chloride cells; development; embryo; gill arches; gill slits; histology; medaka oryzias-latipes; ontogeny; oreochromis-niloticus; paralichthys-olivaceus embryos; rainbow-trout; respiratory development; urinary system; zebrafish  
  Résumé The European sea bass Dicentrarchus labrax is a marine teleost important in Mediterranean aquaculture. The development of the entire digestive tract of D. labrax, including the pharynx, was investigated from early embryonic development to day 5 post hatching (dph), when the mouth opens. The digestive tract is initialized at stage 12 somites independently from two distinct infoldings of the endodermal sheet. In the pharyngeal region, the anterior infolding forms the pharynx and the first gill slits at stage 25 somites. The other three gill arches and slits are formed between 1 and 5 dph. Posteriorly, in the gut tube region, a posterior infolding forms the foregut, midgut and hindgut. The anus opens before hatching, at stage 28 somites. Associated organs (liver, pancreas and gall bladder) are all discernable from 3 dph. Some aspects of the development of the two independent initial infoldings seem original compared with data in the literature. These results are discussed and compared with embryonic and post-embryonic development patterns in other teleosts.  
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  ISSN 0022-1112 ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 543  
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Auteur Ituarte, R.B.; Lignot, J.-H.; Charmantier, G.; Spivak, E.; Lorin-Nebel, C. doi  openurl
  Titre Immunolocalization and expression of Na+/K+ -ATPase in embryos, early larval stages and adults of the freshwater shrimp Palaemonetes argentinus (Decapoda, Caridea, Palaemonidae) Type Article scientifique
  Année 2016 Publication (up) Revue Abrégée Cell Tissue Res.  
  Volume 364 Numéro 3 Pages 527-541  
  Mots-Clés Antennal gland; antennal glands; branchial chamber; crayfish astacus-leptodactylus; dorsal organ; Extrabranchial organs; Gill; h+-atpase; hemigrapsus-crenulatus; kinetic characterization; lobster homarus-gammarus; Ontogeny; Osmoregulation; osmoregulatory organs; prawn macrobrachium-rosenbergii  
  Résumé The euryhaline shrimp Palaemonetes argentinus exemplifies an evolutionary transition from brackish to freshwater habitats that requires adequate osmoregulatory capacities. Hyperosmoregulation is functional at hatching and it likely begins during the embryonic phase allowing this species to develop entirely in fresh water. Here, we investigated the Na+/K+-ATPase alpha-subunit gene (nka-alpha) expression using quantitative real-time PCR and localized Na+/K+-ATPase (NKA) in ion-transporting epithelia through immunofluorescence microscopy. We reared shrimps from spawning to juvenile stages at two salinities (1, 15 aEuro degrees) and maintained adults for 3 weeks at three salinity treatments (1, 15, 25 aEuro degrees). nka-alpha gene expression was measured in: (1) embryos at an early (SI), intermediate (SII) and late (SIII) stage of embryonic development; (2) newly hatched larvae (Zoea I, ZI); and (3) isolated gill tissue of adults. The nka-alpha expression was low in SI and SII embryos and reached maximum levels prior to hatching (SIII), which were similar to expression levels detected in the ZI. The nka-alpha expression in SIII and ZI was highest at 15 aEuro degrees, whereas salinity did not affect expression in earlier embryos. In SIII, in ZI and in a later zoeal stage ZIV, NKA was localized in epithelial cells of pleurae, in the inner-side epithelium of branchiostegite and in the antennal glands. Gills appeared in the ZIV but NKA immunolabeling of the cells of the gill shaft occurred in a subsequent developmental larval stage, the decapodid. Extrabranchial organs constitute the main site of osmoregulation in early ontogenetic stages of this freshwater shrimp.  
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  Langue English Langue du Résumé Titre Original  
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  Volume de collection Numéro de collection Edition  
  ISSN 0302-766x ISBN Médium  
  Région Expédition Conférence  
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  Numéro d'Appel MARBEC @ alain.herve @ collection 1621  
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