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Auteur Lopes Galasso, H.; Callier, M.D.; Bastianelli, D.; Blancheton, J.-P.; Aliaume, C. url  doi
openurl 
  Titre The potential of near infrared spectroscopy (NIRS) to measure the chemical composition of aquaculture solid waste Type Article scientifique
  Année 2017 Publication Revue Abrégée Aquaculture  
  Volume (down) 476 Numéro Pages 134-140  
  Mots-Clés Chemical composition; Dicentrarchus labrax; Faeces; Fish farm waste; Near infrared spectroscopy  
  Résumé In aquaculture, it is extremely important to determine the composition of fish farm waste to evaluate its potential impacts and to improve its reuse. Near-infra red spectroscopy (NIRS), an alternative to standard chemical analytical techniques, is a quick non-invasive method to assess physical and chemical composition, reducing the cost of routine analysis. We developed NIRS calibration models for organic matter (OM), total organic carbon (TOC), total organic nitrogen (TON), the carbon/nitrogen ratio (C/N), total phosphorus (TP) and the lipid content of marine fish particulate waste. To obtain a wide range of compositions of fish waste, decomposition time, feed loss, and inter-specific variations were taken into account. The NIRS calibration models were built using three sub-datasets: in Scenario 1) the calibration was species-specific, including only seabass waste (SeabassWaste), in Scenario 2), the calibration included data from two other species (MultiSpeciesWaste) and in Scenario 3), the general calibration included all data as well as simulation of extreme feed loss (up to 50%) (Faeces&Feed). All calibrations performed using either dried or wet samples gave equations with high coefficients of determination (R2) and reasonably low standard error of cross validation (SECV) values for all parameters tested, except for TP due the high proportion of mineral P.  
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  Langue Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0044-8486 ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2128  
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Auteur Bossus, M.; Charmantier, G.; Blondeau-Bidet, E.; Valletta, B.; Boulo, V.; Lorin-Nebel, C. url  doi
openurl 
  Titre The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax Type Article scientifique
  Année 2013 Publication Revue Abrégée J Comp Physiol B  
  Volume (down) 183 Numéro 5 Pages 641-662  
  Mots-Clés ClC-3 chloride channel; Dicentrarchus labrax; Na+/K+-ATPase; Osmoregulation; Osmosensing  
  Résumé Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na(+)/K(+)-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-alpha1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW, whereas the branchial ClC-3 protein expression was 30 % higher 7 days after the transfer as compared to SW. This is consistent with the increased number of immunopositive MRCs (immunostained for NKA and ClC-3). However, the ClC-3 mRNA expression was significantly lower in FW gills. In the kidney, after FW transfer, a transient decrease in NKA-alpha1 subunit expression was followed by significantly higher stable levels from 24 h. The low ClC-3 protein expression detected at both salinities was not observed by immunocytochemistry in the SW kidney; ClC-3 was localized in the basal membrane of the collecting ducts and tubules 7 and 30 days after transfer to FW. Renal ClC-3 mRNA expression, however, seemed higher in SW than in FW. The potential role of this chloride channel ClC-3 in osmoregulatory and osmosensing mechanisms is discussed.  
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  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0174-1578 ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 888  
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Auteur FERRARI, S.; Leguay, D.; Vergnet, A.; Vidal, M.-O.; Chatain, B.; Bégout, M.-L. url  doi
openurl 
  Titre Unpredictability in food supply during early life influences growth and boldness in European seabass, Dicentrarchus labrax Type Article scientifique
  Année 2016 Publication Revue Abrégée Applied Animal Behaviour Science  
  Volume (down) 180 Numéro Pages 147-156  
  Mots-Clés Behavior; Dicentrarchus labrax; Environmental effect; Exploration; Food deprivation divergent strains; Personality  
  Résumé Biological variability is no longer considered as statistical noise, but rather as an adaptive benefit. This variability comes from consistent differences in behavioral and physiological responses among individuals to a changing/challenging environment, named “coping style”, “temperament” or “personality”. Many studies have described how to characterize personality traits and how to assess their consistency over time and between different contexts; however, little is known about the environmental factors shaping personality development. Because contrasting personalities are maintained with evolution, this lead to the widespread assumption that genes play a predominant role in personality. In many cases, personality traits are however also likely to be determined by individual experience, which is probably at least as important as genetics in shaping personality. The aim of this study was to assess how environmental variability (herein food predictability) impacts behavioral responses, particularly the shyness-boldness axis, one of the most widely shared animal personality trait. Here, we reared juvenile seabass (95 days old) from two divergent strains selected for feed deprivation tolerance under standard conditions for 40 days. Thereafter, we submitted them to two feeding treatments (Predictable versus Unpredictable) starting at 135 dph and lasting 60 days. Seabass reared under a predictable food supply (PFS) grew faster and were shyer than fish reared under an unpredictable food supply (UFS) (i.e. they took more time to exit the refuge zone of a Z-maze; UFS: 132.47 ± 34.63 s; PFS: 336.79 ± 56.97 s) but their exploration tendency was similar. We also examined the behavioral responses of these fish facing a hypoxic challenge. Hypoxia tolerance results were consistent before and after the two feeding treatments. Our findings show the importance of early environmental experience as a driving force shaping boldness. In addition, we provide further evidence that predictable feeding time should be respected in studies assessing essential functions such as growth and behavior. Although personality traits are partially heritable, this study demonstrates the important influence of environmental conditions and life history on behavior.  
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  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0168-1591 ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 1574  
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Auteur Sébastien, F.; Leguay, D.; Vergnet, A.; Vidal, M.-O.; Chatain, B.; Bégout, M.-L. url  doi
openurl 
  Titre Unpredictability in food supply during early life influences growth and boldness in European seabass, Dicentrarchus labrax Type Article scientifique
  Année 2016 Publication Revue Abrégée Applied Animal Behaviour Science  
  Volume (down) 180 Numéro Pages 147-156  
  Mots-Clés Behavior; Dicentrarchus labrax; Environmental effect; Exploration; Food deprivation divergent strains; Personality  
  Résumé  
  Adresse  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0168-1591 ISBN Médium  
  Région Expédition Conférence  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2409  
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Auteur FERRARI, S.; MILLOT, S.; LEGUAY, D.; CHATAIN, B.; BEGOUT, M.-L. url  doi
openurl 
  Titre Consistency in European seabass coping styles: A life history approach Type Article scientifique
  Année 2015 Publication Applied Animal Behaviour Science Revue Abrégée  
  Volume (down) 167 Numéro Pages 74-88  
  Mots-Clés Behavioural plasticity; Repeatability; Life stages; Dicentrarchus labrax  
  Résumé Recent years have seen a growth of interest in the consistent differences in individual behaviour over time and contexts constituting so-called “individual coping styles”. An understanding of this inter-individual variation is essential to improve our knowledge of the adaptive value of behaviour. Coping styles may have implications in diverse fields, so the development of appropriate screening methods for each species appears to be the most effective way to extend our knowledge and to incorporate behavioural responses into selection-based breeding programmes, to improve the domestication and welfare of farmed fish. We tested 30 juvenile seabass (Dicentrarchus labrax) at least twice in individual-based tests (feeding recovery in isolation, aggressiveness, exploration in a T-maze and net restraint) and group-based tests (risk-taking and hypoxia sorting), to assess coping style consistency in the short and long term and between tests. The results of individual-based tests were inconsistent over time and between tests in our set-up: the time between repeat tests, learning and species-specific behavioural responses appeared to have a major impact. By contrast, the results of group-based tests, such as risk-taking and hypoxia sorting, appeared to be consistent (both in the short and long term). These tests therefore appeared to be the most relevant for the characterisation of coping style in European seabass. Furthermore, the results of these tests were also predictive of cortisol stress response. These tests are simple to perform and can be used to screen large numbers of fish, the first step in selection programmes including behavioural profiles  
  Adresse IFREMER, Lab Rech Piscicole Mediterranee Chem Maguelone, Stn Expt Aquaculture, F-34250 Palavas Les Flots, France.  
  Auteur institutionnel Thèse  
  Editeur Elsevier Science Bv Lieu de Publication Éditeur  
  Langue Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0168-1591 ISBN Médium  
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  Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ 36814 collection 1235  
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