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Auteur FERRARI, S.; MILLOT, S.; LEGUAY, D.; CHATAIN, B.; BEGOUT, M.-L.
Titre (up) Consistency in European seabass coping styles: A life history approach Type Article scientifique
Année 2015 Publication Applied Animal Behaviour Science Revue Abrégée
Volume 167 Numéro Pages 74-88
Mots-Clés Behavioural plasticity; Repeatability; Life stages; Dicentrarchus labrax
Résumé Recent years have seen a growth of interest in the consistent differences in individual behaviour over time and contexts constituting so-called “individual coping styles”. An understanding of this inter-individual variation is essential to improve our knowledge of the adaptive value of behaviour. Coping styles may have implications in diverse fields, so the development of appropriate screening methods for each species appears to be the most effective way to extend our knowledge and to incorporate behavioural responses into selection-based breeding programmes, to improve the domestication and welfare of farmed fish. We tested 30 juvenile seabass (Dicentrarchus labrax) at least twice in individual-based tests (feeding recovery in isolation, aggressiveness, exploration in a T-maze and net restraint) and group-based tests (risk-taking and hypoxia sorting), to assess coping style consistency in the short and long term and between tests. The results of individual-based tests were inconsistent over time and between tests in our set-up: the time between repeat tests, learning and species-specific behavioural responses appeared to have a major impact. By contrast, the results of group-based tests, such as risk-taking and hypoxia sorting, appeared to be consistent (both in the short and long term). These tests therefore appeared to be the most relevant for the characterisation of coping style in European seabass. Furthermore, the results of these tests were also predictive of cortisol stress response. These tests are simple to perform and can be used to screen large numbers of fish, the first step in selection programmes including behavioural profiles
Adresse IFREMER, Lab Rech Piscicole Mediterranee Chem Maguelone, Stn Expt Aquaculture, F-34250 Palavas Les Flots, France.
Auteur institutionnel Thèse
Editeur Elsevier Science Bv Lieu de Publication Éditeur
Langue Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0168-1591 ISBN Médium
Région Expédition Conférence
Notes Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ 36814 collection 1235
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Auteur Masroor, W.; Farcy, E.; Blondeau-Bidet, E.; Venn, A.; Tambutte, E.; Lorin-Nebel, C.
Titre (up) Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass Type Article scientifique
Année 2019 Publication Revue Abrégée J. Therm. Biol.
Volume 85 Numéro Pages Unsp-102422
Mots-Clés cftr anion channel; chloride channel; cod gadus-morhua; Dicentrarchus labrax; dicentrarchus-labrax; fresh-water; functional classification; Gill; h+-atpase; Ion transporter; mRNA expression; na+/k+-atpase; na+/k+/2cl(-) cotransporter; Salinity; seawater-acclimated medaka; Thermal acclimation
Résumé The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 degrees C (temperate) or 24 degrees C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase ala (nka ala) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl--cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters involved in ion excretion (Na+/K+/2Cl-1( )cotransporter, nkcc1 and cystic fibrosis transmembrane conductance regulator, cftr) as well as nitrogen excretion (Rh-glycoproteins, rhcg1 and rhbg) and acid-base regulation (V-H+-ATPase, vha-a and b) are highly expressed in SW warm conditions vs FW warm. Overall, our results suggest a higher activation of ion transport processes in warm conditions and more strikingly in SW. This is linked to a strong interplay between diverse ion transporters in order to coordinate physiological responses at the gill level.
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Auteur institutionnel Thèse
Editeur Lieu de Publication Éditeur
Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0306-4565 ISBN Médium
Région Expédition Conférence
Notes WOS:000497246100023 Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 2669
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Auteur Siddique, M.A.M.; Butts, I.A.E.; Linhart, O.; Macias, A.D.; Fauvel, C.
Titre (up) Fertilization strategies for Sea Bass Dicentrarchus labrax (Linnaeus, 1758): effects of pre-incubation and duration of egg receptivity in seawater Type Article scientifique
Année 2015 Publication Revue Abrégée Aquac Res
Volume Numéro Pages 1-9
Mots-Clés Dicentrarchus labrax; egg longevity; Mediterranean sea bass; overripe eggs; reproductive ecology
Résumé Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives of this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min and then fertilized by pooled sperm from four males. The fresh eggs had a higher fertilization success than overripe eggs. Our results revealed a significant effect of pre-incubation time for both the fresh (P < 0.01) and overripe eggs (P < 0.01). Fertilization success of eggs significantly declined for both these treatments after 3 min of pre-incubation, which clearly indicates that sea bass eggs are able to be fertilized by sperm for up to 3 min after release into seawater. This study has particular importance for understanding fertilization strategies, reproductive potential, as well as reproductive ecology of sea bass.
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Auteur institutionnel Thèse
Editeur Lieu de Publication Éditeur
Langue en Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 1365-2109 ISBN Médium
Région Expédition Conférence
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 1456
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Auteur Geay, F.; Culi, E.S.I.; Corporeau, C.; Boudry, P.; Dreano, Y.; Corcos, L.; Bodin, N.; Vandeputte, M.; Zambonino-Infante, J.L.; Mazurais, D.; Cahu, C.L.
Titre (up) Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet Type Article scientifique
Année 2010 Publication Revue Abrégée Comparative Biochemistry and Physiology B-Biochemistry and Molecular Biology
Volume 156 Numéro 4 Pages 237-243
Mots-Clés biosynthesis; FADS2 expression and activity; Hufa; Sea bass (Dicentrarchus labrax)
Résumé Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9 months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol binding regulatory element-binding protein 1 (SREBP-1) mRNA levels, the desaturation rate of [1-C-14]18:3n-3 into [1-C-14]18:4n-3, analysed in microsomal preparations using HPLC method, did not show an upregulation of FADS2 activities in liver and intestine of fish fed VD. Moreover Western-blot analysis did not revealed any significant difference of FADS2 protein amount between the two dietary groups. These data demonstrate that sea bass exhibits a desaturase (FADS2) activity whatever their diet, but a post-transcriptional regulation of fads2 RNA prevents an increase of enzyme in fish fed a HUFA-free diet. This led to a lower fish growth and poor muscle HUFA content.
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Editeur Lieu de Publication Éditeur
Langue Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 1096-4959 ISBN Médium
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Numéro d'Appel LL @ pixluser @ collection 75
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Auteur Bossus, M.; Charmantier, G.; Blondeau-Bidet, E.; Valletta, B.; Boulo, V.; Lorin-Nebel, C.
Titre (up) The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax Type Article scientifique
Année 2013 Publication Revue Abrégée J Comp Physiol B
Volume 183 Numéro 5 Pages 641-662
Mots-Clés ClC-3 chloride channel; Dicentrarchus labrax; Na+/K+-ATPase; Osmoregulation; Osmosensing
Résumé Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na(+)/K(+)-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-alpha1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW, whereas the branchial ClC-3 protein expression was 30 % higher 7 days after the transfer as compared to SW. This is consistent with the increased number of immunopositive MRCs (immunostained for NKA and ClC-3). However, the ClC-3 mRNA expression was significantly lower in FW gills. In the kidney, after FW transfer, a transient decrease in NKA-alpha1 subunit expression was followed by significantly higher stable levels from 24 h. The low ClC-3 protein expression detected at both salinities was not observed by immunocytochemistry in the SW kidney; ClC-3 was localized in the basal membrane of the collecting ducts and tubules 7 and 30 days after transfer to FW. Renal ClC-3 mRNA expression, however, seemed higher in SW than in FW. The potential role of this chloride channel ClC-3 in osmoregulatory and osmosensing mechanisms is discussed.
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Editeur Lieu de Publication Éditeur
Langue English Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 0174-1578 ISBN Médium
Région Expédition Conférence
Notes Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 888
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