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Auteur Masroor, W.; Farcy, E.; Blondeau-Bidet, E.; Venn, A.; Tambutte, E.; Lorin-Nebel, C.
Titre Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass Type Article scientifique
Année 2019 Publication Revue Abrégée J. Therm. Biol.
Volume 85 Numéro Pages Unsp-102422
Mots-Clés cftr anion channel; chloride channel; cod gadus-morhua; Dicentrarchus labrax; dicentrarchus-labrax; fresh-water; functional classification; Gill; h+-atpase; Ion transporter; mRNA expression; na+/k+-atpase; na+/k+/2cl(-) cotransporter; Salinity; seawater-acclimated medaka; Thermal acclimation
Résumé The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 degrees C (temperate) or 24 degrees C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase ala (nka ala) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl--cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters involved in ion excretion (Na+/K+/2Cl-1( )cotransporter, nkcc1 and cystic fibrosis transmembrane conductance regulator, cftr) as well as nitrogen excretion (Rh-glycoproteins, rhcg1 and rhbg) and acid-base regulation (V-H+-ATPase, vha-a and b) are highly expressed in SW warm conditions vs FW warm. Overall, our results suggest a higher activation of ion transport processes in warm conditions and more strikingly in SW. This is linked to a strong interplay between diverse ion transporters in order to coordinate physiological responses at the gill level.
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ISSN 0306-4565 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection (down) 2669
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Auteur Vandeputte, M.; Gagnaire, P.-A.; Allal, F.
Titre The European sea bass: a key marine fish model in the wild and in aquaculture Type Article scientifique
Année 2019 Publication Revue Abrégée Animal Genetics
Volume Numéro Pages
Mots-Clés dicentrarchus labrax; genetics; heritability; population genetics
Résumé The European sea bass (Dicentrarchus labrax L.) is a marine fish of key economic and cultural importance in Europe. It is now more an aquaculture than a fisheries species (>96% of the production in 2016), although modern rearing techniques date back only from the late 1980s. It also has high interest for evolutionary studies, as it is composed of two semispecies (Atlantic and Mediterranean lineages) that have come into secondary contact following the last glaciation. Based on quantitative genetics studies of most traits of interest over the past 10–15 years, selective breeding programs are now applied to this species, which is at the beginning of its domestication process. The availability of a good quality reference genome has accelerated the development of new genomic resources, including SNP arrays that will enable genomic selection to improve genetic gain. There is a need to improve feed efficiency, both for economic and environmental reasons, but this will require novel phenotyping approaches. Further developments will likely focus on the understanding of genotype-by-environment interactions, which will be important both for efficient breeding of farmed stocks and for improving knowledge of the evolution of natural populations. At the interface between both, the domestication process must be better understood to improve production and also to fully evaluate the possible impact of aquaculture escapees on wild populations. The latter is an important question for all large-scale aquaculture productions.
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Volume de collection Numéro de collection Edition
ISSN 1365-2052 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection (down) 2541
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Auteur Maugars, G.; Manirafasha, M.-C.; Grousset, E.; Boulo, V.; Lignot, J.-H.
Titre The effects of acute transfer to freshwater on ion transporters of the pharyngeal cavity in European seabass (Dicentrarchus labrax) Type Article scientifique
Année 2018 Publication Revue Abrégée Fish Physiol. Biochem.
Volume 44 Numéro 5 Pages 1393-1408
Mots-Clés atlantic salmon; atpase alpha-1 isoforms; branchial chloride cells; carbonic-anhydrase; Dicentrarchus labrax; gill na+/k+-atpase; Gills and extrabranchial organs; Ion transporters; k+-atpase; mitochondrion-rich cells; Osmoregulation; salinity transfer; salmon salmo-salar; seawater acclimation; Seawater to freshwater transfer; Teleost fish
Résumé Gene expression of key ion transporters (the Na+/K+-ATPase NKA, the Na+, K+-2Cl(-) cotransporter NKCC1, and CFTR) in the gills, opercular inner epithelium, and pseudobranch of European seabass juveniles (Dicentrarchus labrax) were studied after acute transfer up to 4days from seawater (SW) to freshwater (FW). The functional remodeling of these organs was also studied. Handling stress (SW to SW transfer) rapidly induced a transcript level decrease for the three ion transporters in the gills and operculum. NKA and CFTR relative expression level were stable, but in the pseudobranch, NKCC1 transcript levels increased (up to 2.4-fold). Transfer to FW induced even more organ-specific responses. In the gills, a 1.8-fold increase for NKA transcript levels occurs within 4days post transfer with also a general decrease for CFTR and NKCC1. In the operculum, transcript levels are only slightly modified. In the pseudobranch, there is a transient NKCC1 increase followed by 0.6-fold decrease and 0.8-fold CFTR decrease. FW transfer also induced a density decrease for the opercular ionocytes and goblet cells. Therefore, gills and operculum display similar trends in SW-fish but have different responses in FW-transferred fish. Also, the pseudobranch presents contrasting response both in SW and in FW, most probably due to the high density of a cell type that is morphologically and functionally different compared to the typical gill-type ionocyte. This pseudobranch-type ionocyte could be involved in blood acid-base regulation masking a minor osmotic regulatory capacity of this organ compared to the gills.
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Langue English Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 0920-1742 ISBN Médium
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Numéro d'Appel MARBEC @ alain.herve @ collection (down) 2417
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Auteur Sébastien, F.; Leguay, D.; Vergnet, A.; Vidal, M.-O.; Chatain, B.; Bégout, M.-L.
Titre Unpredictability in food supply during early life influences growth and boldness in European seabass, Dicentrarchus labrax Type Article scientifique
Année 2016 Publication Revue Abrégée Applied Animal Behaviour Science
Volume 180 Numéro Pages 147-156
Mots-Clés Behavior; Dicentrarchus labrax; Environmental effect; Exploration; Food deprivation divergent strains; Personality
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Langue English Langue du Résumé Titre Original
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Volume de collection Numéro de collection Edition
ISSN 0168-1591 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection (down) 2409
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Auteur Lopes Galasso, H.; Callier, M.D.; Bastianelli, D.; Blancheton, J.-P.; Aliaume, C.
Titre The potential of near infrared spectroscopy (NIRS) to measure the chemical composition of aquaculture solid waste Type Article scientifique
Année 2017 Publication Revue Abrégée Aquaculture
Volume 476 Numéro Pages 134-140
Mots-Clés Chemical composition; Dicentrarchus labrax; Faeces; Fish farm waste; Near infrared spectroscopy
Résumé In aquaculture, it is extremely important to determine the composition of fish farm waste to evaluate its potential impacts and to improve its reuse. Near-infra red spectroscopy (NIRS), an alternative to standard chemical analytical techniques, is a quick non-invasive method to assess physical and chemical composition, reducing the cost of routine analysis. We developed NIRS calibration models for organic matter (OM), total organic carbon (TOC), total organic nitrogen (TON), the carbon/nitrogen ratio (C/N), total phosphorus (TP) and the lipid content of marine fish particulate waste. To obtain a wide range of compositions of fish waste, decomposition time, feed loss, and inter-specific variations were taken into account. The NIRS calibration models were built using three sub-datasets: in Scenario 1) the calibration was species-specific, including only seabass waste (SeabassWaste), in Scenario 2), the calibration included data from two other species (MultiSpeciesWaste) and in Scenario 3), the general calibration included all data as well as simulation of extreme feed loss (up to 50%) (Faeces&Feed). All calibrations performed using either dried or wet samples gave equations with high coefficients of determination (R2) and reasonably low standard error of cross validation (SECV) values for all parameters tested, except for TP due the high proportion of mineral P.
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Volume de collection Numéro de collection Edition
ISSN 0044-8486 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection (down) 2128
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