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Auteur (up) Mazurais, D.; Covès, D.; Papandroulakis, N.; Ortega, A.; Desbruyeres, E.; Huelvan, C.; Le Gall, M.M.; de la Gándara, F.; Cahu, C.L.
Titre Gene expression pattern of digestive and antioxidant enzymes during the larval development of reared Atlantic bluefin tuna (ABFT), Thunnus thynnus L Type Article scientifique
Année 2015 Publication Revue Abrégée Aquac Res
Volume 46 Numéro 10 Pages 2323-2331
Mots-Clés antioxidant; development; digestion; expression; gene; Tuna
Résumé The aim of this study was to determine whether mortality observed during the larval development of reared bluefin tuna (Thunnus thynnus) could be related to improper expression profiles of key genes involved in digestive or antioxidant response capabilities. Tuna larvae were sampled at hatching, 2, 5, 10, 15 and 20 dph (days post hatching) for the relative quantification of transcripts encoded by genes involved in digestive [trypsinogen 1 (TRYP1), alpha-amylase (AMY), aminopeptidase N (ANPEP)] and antioxidant [catalase (CAT)] functions. The levels of expression of ANPEP related to the development and maturation of intestinal function increased from 5 to 20 dph. Furthermore, AMY and TRYP1 genes, which are pancreatic enzymes implicated in carbohydrate and peptide digestions exhibit a typical peak of expression at 5 and 15 dph respectively. The antioxidant enzyme, CAT, exhibited higher mRNA levels during the first stage of larval development. In conclusion, our investigation indicates that the expression of genes involved in digestive and antioxidant physiological processes followed typical patterns which could not explain high mortality rate observed during the first stage of larval development.
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ISSN 1365-2109 ISBN Médium
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Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 1455
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Auteur (up) Siddique, M.A.M.; Butts, I.A.E.; Linhart, O.; Macias, A.D.; Fauvel, C.
Titre Fertilization strategies for Sea Bass Dicentrarchus labrax (Linnaeus, 1758): effects of pre-incubation and duration of egg receptivity in seawater Type Article scientifique
Année 2015 Publication Revue Abrégée Aquac Res
Volume Numéro Pages 1-9
Mots-Clés Dicentrarchus labrax; egg longevity; Mediterranean sea bass; overripe eggs; reproductive ecology
Résumé Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives of this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min and then fertilized by pooled sperm from four males. The fresh eggs had a higher fertilization success than overripe eggs. Our results revealed a significant effect of pre-incubation time for both the fresh (P < 0.01) and overripe eggs (P < 0.01). Fertilization success of eggs significantly declined for both these treatments after 3 min of pre-incubation, which clearly indicates that sea bass eggs are able to be fertilized by sperm for up to 3 min after release into seawater. This study has particular importance for understanding fertilization strategies, reproductive potential, as well as reproductive ecology of sea bass.
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Auteur institutionnel Thèse
Editeur Lieu de Publication Éditeur
Langue en Langue du Résumé Titre Original
Éditeur de collection Titre de collection Titre de collection Abrégé
Volume de collection Numéro de collection Edition
ISSN 1365-2109 ISBN Médium
Région Expédition Conférence
Notes Approuvé pas de
Numéro d'Appel MARBEC @ isabelle.vidal-ayouba @ collection 1456
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